Savita Yadav - Academia.edu (original) (raw)

Papers by Savita Yadav

Der Pharmacia Lettre, 2016

Pseudoephedrine is used for the temporary relief of stuffy nose and sinus pain/pressure caused by... more Pseudoephedrine is used for the temporary relief of stuffy nose and sinus pain/pressure caused by infection such as the common cold and flu. Here we were reporting a simple and precise validated stability indicating RP-HPLC method for estimation of Pseudoephedrine Sulfate and its related compounds in solid oral dosage forms. The stability indicating nature of current method has been demonstrated by a forced degradation study. All process related impurities and degradation products from forced degradation samples were well resolved from each other. The chromatographic separation of impurities was achieved with a Hypersil phenyl column (25 cm x 4.6 mm i.d., particle size 5 μ) using buffer and acetonitrile in the ratio of 95:5 v/v respectively as the mobile phase. The buffer used in mobile phase contained 10 mM potassium dihydrogen phosphate and its pH was adjusted to 3.0 ±0.05 with diluted orthophosphoric acid. The flow rate of HPLC was 1.0 mL min-1 and the wavelength of UV detector at 210 nm. The method was shown a linear response over range of 0.125 μg mL-1 to 0.750 μg mL-1 PES and impurities. The correlation coefficient was more than 0.999. Performed the robustness by using design experiments.

Der Pharma Chemica, 2011

... Vishal V Bharekar, Toufik S Mulla, Savita S Yadav, Milind P Rajput and Janhavi R Rao* ... 2. ... more ... Vishal V Bharekar, Toufik S Mulla, Savita S Yadav, Milind P Rajput and Janhavi R Rao* ... 2. Lakshmana AR, Ravi Kumar BNV and Sankar GG; Development of RP-HPLC Method for the Estimation of Rabeprazole in Pure and Tablet Dosage Form. E-Journal of Chemistry. ...

Research Journal of Pharmacy and Technology, Mar 28, 2010

Asian Journal of Pharmaceutical and Clinical Research, Mar 1, 2017

Objective: The objective of this study was to develop and validate a simple, precise and accurate... more Objective: The objective of this study was to develop and validate a simple, precise and accurate reverse phase high performance liquid chromatographic method for simultaneous estimation of dicyclomine hydrochloride, paracetamol, and mefenamic acid from multicomponent tablet dosage form. Methods: The optimized chromatographic separation was achieved using a stationary phase of hypersil gold ODS-C18 (250 × 4.6 mm i.d, 5 μm particle size) column and mobile phase of acetonitrile: Methanol: Potassium dihydrogen phosphate buffer, pH adjusted to 4.5 with orthophosphoric acid (OPA) in the ratio of 50: 30: 20 (v/v/v) with a flow rate of 1 mL/minute. The ultraviolet detection was carried out at 260 nm. Results: The retention times of paracetamol, dicyclomine hydrochloride and MEF were found to be 4.2, 6.1 and 3.4 minutes, respectively. The method was validated for linearity, precision, accuracy, and robustness. The developed method provided linear responses within the concentration ranges 2-12 µg/mL for dicyclomine hydrochloride, 50-300 µg/mL for paracetamol, and 25-150 µg/mL for MEF. The % recovery obtained were found to be 99.09-99.44% for dicyclomine hydrochloride, 99.22-99.51% for paracetamol, and 99.49-99.60 for MEF. Conclusion: The proposed method enables rapid quantification and simultaneous analysis of all the drugs from commercial formulations without any interference of excipients. Hence, the developed method can be successfully applied for routine analysis of dicyclomine hydrochloride, paracetamol, and MEF in their combined tablet formulation.

Jpc – Journal Of Planar Chromatography – Modern Tlc, Apr 1, 2011

ABSTRACT

Objective: The present study was designed with an objective of a simple, fast, precise, selective... more Objective: The present study was designed with an objective of a simple, fast, precise, selective and accurate HPTLC method was developed and validated for the simultaneous determination of bisoprolol fumarate and hydrochlorothiazide from bulk and formulations. Methods: High performance thin layer chromatography (HPTLC) method was developed and validated for rapid analysis of simultaneous determination of bisoprolol fumarate and hydrochlorothiazide. Chromatographic separation was achieved on precoated silica gel HPTLC aluminum plate 60 F254 using ethyl acetate: methanol: ammonia 10:0.5:0.5 (v/v) as mobile phase. Detection was performed at 225 nm wavelength densitometrically. Results: The RF of bisoprolol fumarate and hydrochlorothiazide were 0.60 and 0.38, respectively. Linearity was observed in the concentration range of 150-900 ng/spot for bisoprolol fumarate and 100-600 ng/spot for hydrochlorothiazide. Percent recoveries obtained for both the drugs were 99.45 ± 0.06% for bisoprolol fumarate and 100.25 ± 1.20 % for hydrochlorothiazide, respectively. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. Conclusion: The method developed can be used for the routine analysis of bisoprolol fumarate and hydrochlorothiazide from their combined dosage form.

Asian Journal of Research in Chemistry, 2012

A rapid assay procedure have been developed for the determination of Nebivolol hydrochloride base... more A rapid assay procedure have been developed for the determination of Nebivolol hydrochloride based on application of oxidant to the spectrophotometry and high performance thin layer chromatography (HPTLC) have been developed for simultaneous determination of Nebivolol hydrochloride and Hydrochlorothiazide in a bulk drug and pharmaceutical formulation. In spectrophotometric method, Nebivolol Hydrochloride is treated with a known excess of cerium (IV) sulphate and the residual oxidant is determined by treating with a fixed amount of indigo carmine, and measuring the absorbance at 610 nm and Chromatographic separation was achieved on aluminum foil plates precoated with silica gel 60GF-254, with chloroform: toluene: methanol: ammonia (5:3:2:0.1, v/v/v/v) as mobile phase. Detection was performed densitometrically at 278 nm. The RF of Nebivolol and Hydrochlorothiazide were 0.30 and 0.42, respectively. In spectrophotometry, Beer's law is obeyed over concentration range of 2–10 μg/mL. T...

Micellar liquid chromatographic method has been developed for the simultaneous determination of P... more Micellar liquid chromatographic method has been developed for the simultaneous determination of Propranolol hydrochloride and Hydrochlorothiazide from bulk and formulations. Chromatographic separation achieved isocratically on ODS hypersil C18 column (5 μm, 250 mm × 4.6 mm) and micellar mobile phase of 0.07M sodium dodecyl sulfate (SDS) pH 3 adjusted with phosphate buffer and 15 % (v/v) 1- propanol as organic modifier in the ratio 70: 30 v/v and ultraviolet detection at 274 nm are used for the determination. In the developed method Propranolol hydrochloride and Hydrochlorothiazide elute at typical retention times of 6.767 min and 2.633 min, respectively at a 1 mL/min flow rate. Parameters such as linearity, precision, accuracy, specificity and robustness are studied as reported in the ICH guidelines. Linearity was observed in the concentration range of 4-24 μg/mL for Propranolol hydrochloride and and 2.5-15 μg/mL for Hydrochlorothiazide. The mean recoveries obtained for Propranolol ...

A fast, simple, precise, accurate and robust HPTLC method was developed for simultaneous determin... more A fast, simple, precise, accurate and robust HPTLC method was developed for simultaneous determination of paracetamol and dexketoprofen trometamol in tablet dosage form. Silica gel G 60F 254 plates were used as stationary phase and toluene: ethyl acetate: acetic acid (6: 4: 0.2 v/v/v) as mobile phase. Wavelength selected for analysis was 256 nm. The two drugs were satisfactorily resolved with R f 0.20 ± 0.02 and 0.55 ± 0.02 for paracetamol and dexketoprofen trometamol respectively. Linearity was found to be in the range of 25-150 ng/spot for paracetamol, 100-600 ng/spot for dexketoprofen trometamol. Intra-day and inter-day precision were found to be (%RSD) 0.94–1.58 % and 1.14–1.83 % for paracetamol and (%RSD) 0.67–1.03 % and 0.49–1.18 for dexketoprofen trometamol. Accuracy of paracetamol and dexketoprofen trometamol was found to be 99.91 % and 99.56 %, respectively. The proposed method can be used for routine simultaneous analysis of paracetamol and dexketoprofen trometamol.

International Journal of Pharmacy and Pharmaceutical Sciences

Objective: The present study aimed to develop and validate the simultaneous estimation of Salbuta... more Objective: The present study aimed to develop and validate the simultaneous estimation of Salbutamol and Ketotifenin tablet dosage form. Methods: An isocratic reversed phase high-performance liquid chromatographic (HPLC) method with ultraviolet detection at 280nm has been developed for the determination of Salbutamol and Ketotifen in dosage formulation. Results: Good chromatographic separation was achieved by using a Thermo Hypersil Gold ODS–C Original Article SIMULTANEOUS ESTIMATION OF SALBUTAMOL AND KETOTIFEN IN TABLET DOSAGE FORM BY RP-HPLC USING ULTRAVIOLET DETECTION AND ITS APPLICATION FOR DISSOLUTION STUDY SNEHAL CHOUDHARI, SAVITA S YADAV, JANHAVI R RAO* Department of Quality Assurance Techniques, BharatiVidyapeeth Deemed University, Poona College of Pharmacy Erandwane, Pune 411038, India. Email: raojanhavi@rediffmail.com 18 (250 mm × 4.6 mm, 5.0 μm). The system was operated at ambient temperature (25 ± 2°C) using a mobile phase consisting of Methanol: KH2PO4 Keywords: Salbuta...

sphinxsai.com

Abstract: A new simple, precise, accurate, and selective high performance thin-layer chromatograp... more Abstract: A new simple, precise, accurate, and selective high performance thin-layer chromatographic (HPTLC) method has been developed for simultaneous analysis of tenofovir and emtricitabine in a tablet dosage form. Chromatographic separation was ...

High performance thin layer chromatographic method has been developed for the simultaneous determ... more High performance thin layer chromatographic method has been developed for the simultaneous determination of Propranolol hydrochloride and Hydrochlorothiazide from bulk and formulations. Chromatographic separation was achieved on aluminum foil plates precoated with silica gel 60F254, with chloroform: ethyl acetate: methanol 4: 4: 2 (v/v/v) as mobile phase. Detection was performed densitometrically at 274 nm. The Rf of Propranolol hydrochloride and Hydrochlorothiazide were 0.27± 0.02 and 0.56 ± 0.02, respectively. Parameters such as linearity, precision, accuracy, specificity and robustness are studied as reported in the ICH guidelines. Linearity was observed in the concentration range of 160-960 ng/band for Propranolol hydrochloride and 100-600 ng/band for Hydrochlorothiazide. The mean recoveries obtained for Propranolol hydrochloride and Hydrochlorothiazide were 99.63 % and 99.15 % respectively. Developed method was found to be accurate, precise, selective and rapid for simultaneous...