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Papers by Somboon Tanasupawat

International journal of systematic and evolutionary microbiology, Jan 6, 2015

A new Actinoplanes species, strain LDG1-22T for which we propose the name Actinoplanes lichenis s... more A new Actinoplanes species, strain LDG1-22T for which we propose the name Actinoplanes lichenis sp. nov., was isolated from a lichen sample collected from the tree bark in Thailand. The taxonomic position of the species was described based on the polyphasic approach. Strain LDG1-22T produced irregular sporangia on agar media. It contained meso-diaminopimelic acid in cell-wall peptidoglycan. Major menaquinone was MK-9(H4). Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and phosphatidylglycerol. Whole-cell hydrolysates contained ribose, glucose, mannose and small amount of arabinose and xylose. The major cellular fatty acids were anteiso-C15:0 (31.2%) and iso-C16:0 (14.2%). Mycolic acids were absent. The G+C content was 73.6%. The 16S rRNA gene analysis of strain LDG1-22T showed the highest similarity (98.8%) to Actinoplanes friuliensis DSM 45797T and shared the cluster together with Actinoplanes nipponensis JC...

Thirty five strains of Streptomyces from 24 soil samples collected in Krabi, Chaiyaphoom, Chantha... more Thirty five strains of Streptomyces from 24 soil samples collected in Krabi, Chaiyaphoom, Chanthaburi, Chonburi, Lampang, Kanchanaburi, Nongkhai, Ratchaburi, Chachoengsao, and Srakaew provinces in Thailand were screened for antimicrobial activities. Thirty strains exhibited antibacterial activity against Bacillus subtilis ATCC 6633, 15 strains inhibited Staphylococcus aureus ATCC 25933, 3 strains inhibited Escherichia coli ATCC 25922 and only a strain CB5-3 showed

In the course of identification and screening for antimicrobial activities of 100 actinomycete is... more In the course of identification and screening for antimicrobial activities of 100 actinomycete isolates from 35 soil and sand samples collected from the shore of Samed Island, Rayong province, 79 isolates were identified as Streptomyces, 20 were Micromonospora, and one was Nocardia based on their morphological, cultural, physiological and biochemical characteristics. On primary screening for antimicrobial activities, 54 isolates of Streptomyces, 14 of Micromonospora, and one of Nocardia showed the activities against Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, and Candida albicans ATCC 10231, while the activities against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 could be observed in few isolates. On secondary screening of 30 isolates, the strain PC4 -3 with good antimicrobial activity was selected for secondary metabolite fermentation. This strain contained LL -diaminopemelic acid in cell wall and was similar to Streptomyces sporoclivatus LMG 20312 T with 99.1% 16S rDNA sequence similarity. In addition, the representative strains of genera, MS7 -3 and SC36 -1 were closed to M. matsumotoense JCM 9104 T and Nocardia araoensis DSM 44729 T based on 98.6% and 99.3% of 16S rDNA sequence similarity, respectively. The ethyl acetate extract from fermentation broth of PC4 -3 showed antimicrobial activities against S. aureus ATCC 25923, B. subtilis ATCC 6633 and C. albicans ATCC 10231. Antimicrobial assay -guided fractionation of the ethyl acetate extract yielded 2 known ansamycins, including geldanamycin and 17 -O -demethylgeldanamycin. The chemical structures of the isolated compounds were elucidated through extensive analyses of UV, IR, MS and NMR spectroscopic data and comparison with the literatures. Geldanamycin exhibited antimicrobial activity against S. aureus ATCC 25923 and C. albicans ATCC 10231.

International journal of systematic and evolutionary microbiology, Jan 27, 2015

A novel actinomycete strain RCU-197T was isolated from peat swamp forest soil collected from Rayo... more A novel actinomycete strain RCU-197T was isolated from peat swamp forest soil collected from Rayong Province, Thailand. Based on polyphasic approach, the strain was classified in the genus Steptomyces. It contained LL-diaminopimelicacid in cell wall peptidoglycan. No diagnostic sugars was detected in whole cell hydrolysates and lack of mycolic acids. The major menaquinones were MK-9(H6) and MK-9(H8). The predominant cellular fatty acids were iso-C14:0, iso-C15:0, anteiso-C15:0 and iso-C16:0. The polar lipids profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannoside. On the basis of the phylogenetic analysis of 16S rRNA gene, the strain formed distinct clade within the genus Streptomyces and was closely related to Streptomyces echinatusNBRC 12763T (98.78% similarity). According to the polyphasic approach as well as DNA-DNA relatedness, the strain could be clearly differentiated from its closely...

Bioscience Biotechnology and Biochemistry

In order to find a unique proteinase, proteinase-producing bacteria were screened from fish sauce... more In order to find a unique proteinase, proteinase-producing bacteria were screened from fish sauce in Thailand. An isolated moderately halophilic bacterium was classified and named Filobacillus sp. RF2-5. The molecular weight of the purified enzyme was estimated to be 49 kDa. The enzyme showed the highest activity at 60 degrees C and pH 10-11 under 10% NaCl, and was highly stable in the presence of about 25% NaCl. The activity was strongly inhibited by phenylmethane sulfonyl fluoride (PMSF), chymostatin, and alpha-microbial alkaline proteinase inhibitor (MAPI). Proteinase activity was activated about 2-fold and 2.5-fold by the addition of 5% and 15-25% NaCl respectively using Suc-Ala-Ala-Phe-pNA as a substrate. The N-terminal 15 amino acid sequence of the purified enzyme showed about 67% identity to that of serine proteinase from Bacillus subtilis 168 and Bacillus subtilis (natto). The proteinase was found to prefer Phe, Met, and Thr at the P1 position, and Ile at the P2 position of ...

Journal of Food Science, 2015

Staphylococcus sp. CMC5-3-1 and CMS5-7-5 isolated from fermented fish sauce at 3 to 7 mo, respect... more Staphylococcus sp. CMC5-3-1 and CMS5-7-5 isolated from fermented fish sauce at 3 to 7 mo, respectively, showed different characteristics on protein hydrolysis and volatile formation. These Gram-positive cocci were able to grow in up to 15% NaCl with the optimum at 0.5% to 5% NaCl in tryptic soy broth. Based on ribosomal 16S rRNA gene sequences, Staphylococcus sp. CMC5-3-1 and CMS5-7-5 showed 99.0% similarity to that of Staphylococcus piscifermentans JCM 6057 T , but DNA-DNA relatedness was <30%, indicating that they were likely to be new species. DNA relatedness between these 2 strains was only 65%, suggesting that they also belonged to different species. The α-amino group content of 6-month-old fish sauce inoculated with Staphylococcus sp. CMC5-3-1 was 740.5 mM, which was higher than that inoculated by the strain CMS5-7-5 (662.14 mM, P < 0.05). Histamine was not produced during fermentations with both strains. Fish sauce inoculated with Staphylococcus sp. CMC5-3-1 showed the highest content of total glutamic acid (P < 0.05). The major volatile compound detected in fish sauce inoculated with Staphylococcus sp. CMC5-3-1 was 2-methypropanal, contributing to the desirable dark chocolate note. Staphylococcus sp. CMC5-3-1 could be applied as a starter culture to improve the umami and aroma of fish sauce.

International Journal of Systematic and Evolutionary Microbiology, 2015

The novel actinomycete strain PWB-003T, which produced fluostatins B and C antibiotics, was isola... more The novel actinomycete strain PWB-003T, which produced fluostatins B and C antibiotics, was isolated from near-shore sediment collected from Panwa cape, Phuket Province, Thailand. Based on the polyphasic approach, strain PWB-003T showed identical characteristics to those members of the genus Micromonospora. It produced single spores on substrate mycelia and contained meso-diaminopimelic acid in cell wall peptidoglycan. Whole-cell hydrolysate contained ribose, xylose, arabinose, mannose and glucose. The predominant menaquinone was MK-10 (H4). Cellular fatty acids comprised C18:1ω9c, iso-C16:0, anteiso-C17:0, iso-C15:0 and iso-C17:0. According to 16S rRNA gene similarity analysis, the strain was closely related to Micromonospora eburnea LK2-10T (99.38%), Micromonospora chaiyaphumensis MC5-1T (99.16%), Micromonospora yangpuensis JCM 18319T (98.97%), Micromonospora echinaurantiaca JCM 3257T (98.97%), Micromonospora pallida JCM 3133T (98.97%), Micromonospora sagamiensis JCM 3310T and Micromonospora auratinigra TT1-11T (98.97%). The G+C content of DNA was 74.5 mol %. DNA-DNA relatedness values among strain PWB-003T and related type strains ranged from 11.3±1.3% to 38.8±1.1%. On the basis of these observation mentioned, strain PWB-003T could be distinguished from its closely related type strains and represents a novel species of the genus Micromonospora, for which the name Micromonospora fluostatini sp. nov. (type strain PWB-003T = JCM 30529T = PCU 341T = TISTR 2345T) is proposed.

Two actinomycetes strains, JSM5-1 and JSM1-3 were isolated from sea sands collected in Chumporn a... more Two actinomycetes strains, JSM5-1 and JSM1-3 were isolated from sea sands collected in Chumporn and in Prachuapkhirikhun provinces, respectively. On the basis of morphological, cultural, physiological and biochemical characteristics, and 16S rDNA sequences analyses, they were identified as Micromonospora marina. The antibiotic fermentation of strains in different liquid media, pHs, and temperatures, revealed that those cultivated in glucose soybean (GS) medium at pH 7.0 and at 28 ºC, showed high antibacterial activities. The ethyl acetate extract of fermentation broth showed an antibacterial activity against Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, and Escherichia coli ATCC 25922. Through the use of several chromatographic techniques, the compounds from fermentation broth of JSM5-1 were separated and identified. Furthermore the fermentation and antibacterial activity of JSM5-1 in glycerol molasses peptone (GP) medium was carried out. The extraction and biologic...

Tropical biomedicine

The aim of this research was to study on the identification and antimicrobial activity of actinob... more The aim of this research was to study on the identification and antimicrobial activity of actinobacteria from six soil samples collected around Krung Ching waterfall, Nakhon Si Thammarat province, the southern part of Thailand. Thirty-one isolates of actinobacteria were isolated using the dilution plating method on modified starch casein nitrate agar plates and potato starch-glycerol agar plates. On the primary screening, 9 isolates exhibited the antimicrobial activity against Bacillus subtilis, 8 isolates against Kocuria rhizophila, 6 isolates against Mucor racemosus, 2 isolates against Escherichia coli and Candida albicans and 5 isolates against Xanthomonas campestris pv. oryzae. All the isolates were identified based on their morphological and cultural characteristics including the 16S rRNA gene sequence analysis. Eighteen isolates were identified as Streptomyces, 8 isolates as Nocardia, 2 isolates as Kitasatospora, one of each isolate as Amycolatopsis, Rhodococcus and Gordonia.

International Journal of Systematic and Evolutionary Microbiology

Three strains, RBY-1(T), PHD-1 and PHD-2, were isolated from fruits in Thailand. The strains were... more Three strains, RBY-1(T), PHD-1 and PHD-2, were isolated from fruits in Thailand. The strains were Gram-negative, aerobic rods with polar flagella, produced acetic acid from ethanol and did not oxidize acetate or lactate. In phylogenetic trees based on 16S rRNA gene sequences and 16S-23S rRNA gene internal transcribed spacer (ITS) sequences, the strains formed a cluster separate from the type strains of recognized species of the genus Gluconobacter. The calculated 16S rRNA gene sequence and 16S-23S rRNA gene ITS sequence similarities were respectively 97.7-99.7 % and 77.3-98.1 %. DNA G+C contents ranged from 57.2 to 57.6 mol%. The strains showed high DNA-DNA relatedness of 100 % to one another, but low DNA-DNA relatedness of 11-34 % to the tested type strains of recognized Gluconobacter species. Q-10 was the major quinone. On the basis of the genotypic and phenotypic data obtained, the three strains clearly represent a novel species, for which the name Gluconobacter nephelii sp. nov....

Twenty-six strains of acetic acid bacteria were isolated from fruits, flowers and related materia... more Twenty-six strains of acetic acid bacteria were isolated from fruits, flowers and related materials collected in Thailand. They were divided into three genera, Acetobacter, Gluconobacter and Asaia, by phenotypic characterization and 16S rRNA gene sequence analyses. On the basis of 16S-23S rRNA gene internal transcribed spacer (16S-23S rDNA ITS) restriction and 16S rRNA gene sequence analyses, fourteen isolates assigned to the genus Acetobacter were divided into five groups: 1) Group 1A or A. tropicalis (one isolate); 2) Group 2A or A. orientalis (four isolates); 3) Group 3A or A. pasteurianus (five isolates); 4) Group 4A or A. syzygii (one isolate); and 5) Group 5A or A. ghanensis (three isolates). The eleven isolates assigned to the genus Gluconobacter were divided into three groups: 6) Group 1B or G. frateurii (four isolates); 7) Group 2B or G. japonicus (six isolates); and 8) Group 3B or unidentified (one isolate). The remaining isolate was placed into: 9) Group 1C or unidentified, which was assigned to the genus Asaia and considered to constitute a new species on the basis of the 16S rRNA gene sequence analysis and DNA-DNA hybridization.

Microbes and Environments

Annals of Microbiology

Ninety-seven acetic acid bacteria, which were isolated from fruits, flowers and other material co... more Ninety-seven acetic acid bacteria, which were isolated from fruits, flowers and other material collected in Thailand by an enrichment culture approach, were assigned to the genusAcetobacter by phenotypic and chemotaxonomic characterisations. On the basis of 16S–23S rRNA gene ITS restriction and 16S rRNA gene sequence analyses, the ninety-seven isolates were grouped into three groups and identified at the specific level: 1) group A including fifty-three isolates, which were identified asAcetobacter pasteurianus, 2) group B including forty-two isolates, which were identified asAcetobacter orientalis and 3) group C including two isolates, which were identified asAcetobacter lovaniensis. There was no isolate to be assigned to other 15 species of the genusAcetobacter.

Four isolates, isolated from fruit of sapodilla collected at Chantaburi and designated as CT8-1 a... more Four isolates, isolated from fruit of sapodilla collected at Chantaburi and designated as CT8-1 and CT8-2, and isolated from seeds of ixora ("khem" in Thai, Ixora species) collected at Rayong and designated as SI15-1 and SI15-2, were examined taxonomically. The four isolates were selected from a total of 181 isolated acetic acid bacteria. Isolates CT8-1 and CT8-2 were non motile and produced a levan-like mucous polysaccharide from sucrose or D-fructose, but did not produce a water-soluble brown pigment from D-glucose on CaCO 3 -containing agar slants. The isolates produced acetic acid from ethanol and oxidized acetate and lactate to carbon dioxide and water, but the intensity of the acetate and lactate oxidation was weak. Their growth was not inhibited by 0.35 % acetic acid (v/v) at pH 3.5. The isolates did not grow on 30 % D-glucose (w/v), and utilization of methanol was not found. Isolates SI15-1 and SI15-2 had peritrichous flagella and grew in the presence of either 0.35 % acetic acid (v/v) at pH 3.5, 3 % NaCl (w/v), or 1 % KNO 3 (w/v). Acetate and lactate were oxidized to carbon dioxide and water, but the intensity was weak. The isolates grew on mannitol agar and glutamate agar as well as on 30 % Dglucose (w/v), but did not utilize methanol. The 16S rRNA gene sequence analysis and DNA-DNA hybridization indicated that isolates CT8-1 and CT8-2 and isolates SI15-1 and SI15-2 were unequivocally identified respectively as Kozakia baliensis and Swaminathania salitolerans.

Journal of the Science of Food and Agriculture

Fermented rice flour (khao-khab, a non-glutinous rice) and related products are Thai traditional ... more Fermented rice flour (khao-khab, a non-glutinous rice) and related products are Thai traditional products. The types of acetic acid bacteria (AAB) microflora in khao-khab have not been reported. In this study, Acetobacter strains were isolated and identified based on the phenotypic and chemotaxonomic characteristics and molecular aspects. Twenty-five acetic acid bacteria isolated from fermented rice products and a starter for sweetened rice in Thailand by an enrichment culture approach, were assigned to the genus Acetobacter by phenotypic and chemotaxonomic characterisations. On the basis of the 16S rRNA gene sequence and 16S-23S rRNA gene ITS restriction analyses, 25 isolates were divided into six groups and identified at the specific level: (1) Group 1 included five isolates, which were identified as A. indonesiensis; (2) Group 2 included two isolates, which were identified as A. lovaniensis; (3) Group 3 included one isolate, which was identified as A. orientalis; (4) Group 4 incl...

Three strains, RBY-1T, PHD-1, and PHD-2 were isolated from fruits in Thailand. The strains were G... more Three strains, RBY-1T, PHD-1, and PHD-2 were isolated from fruits in Thailand. The strains were Gram-negative, aerobic rods with polar flagella, produced acetic acid from ethanol and did not oxidize acetate and lactate. In phylogenetic trees based on 16S rRNA gene sequences and 16S-23S rRNA gene ITS sequences, the strains formed a cluster separate from the type strains of the eleven species of the genus Gluconobacter. The calculated 16S rRNA gene sequence and 16S-23S rRNA gene ITS sequence similarities were respectively 97.7-99.7% and 77.3-98.1%. DNA G+C contents were 57.2-57.6 mol%. They showed high DNA-DNA relatedness of 100% with one another but low DNA-DNA relatedness of 11-34% with the type strains of the eleven Gluconobacter species. Q-10 was a major quinone. On the basis of the genotypic and phenotypic data obtained, the three strains clearly represent a novel species, for which the name Gluconobacter nephelii sp. nov. is proposed. The type strain is strain RBY-1T (= BCC 3673...

The Journal of General and Applied Microbiology

... J. Gen. Appl. Microbiol., 56, 67 73. Yukphan, P., Malimas, T., Muramatsu, Y., Potacharoen, W.... more ... J. Gen. Appl. Microbiol., 56, 67 73. Yukphan, P., Malimas, T., Muramatsu, Y., Potacharoen, W.,Tana-supawat, S., Nakagawa, Y., Tanticharoen, M., and Yamada, Y. (2011) Neokomagataea gen. nov., with descriptions of Neokomagataea thailandica sp. nov. ...

International journal of systematic and evolutionary microbiology, Jan 28, 2015

Three strains of Lactobacillus, NB53T, NB446T, and NB702 were isolated from traditional fermented... more Three strains of Lactobacillus, NB53T, NB446T, and NB702 were isolated from traditional fermented food in Thailand. Comparative 16S rRNA gene sequence analysis indicated that these strains belong to the Lactobacillus plantarum group. Phylogenetic analysis based on the dnaK, rpoA, pheS, and recA gene sequences indicated that these three strains were distantly related to known species present in the Lactobacillus plantarum group. DNA-DNA hybridization with closely related strains demonstrated that these strains represented two novel species; these strains were differentiated based on chemotaxonomical and phenotypic characteristics. Therefore, two novel Lactobacillus species, Lactobacillus plajomi sp. nov. (NB53T) and Lactobacillus modestisalitolerans sp. nov. (NB446T and NB702), are proposed with the type strains NB53T (= NBRC 107333T = BCC 38054T) and NB446T (= NBRC 107235T = BCC 38191T), respectively.

International journal of systematic and evolutionary microbiology, 2014

Two actinomycete strains, KK1-2(T) and CPB4-7, were isolated from marine sediments collected in C... more Two actinomycete strains, KK1-2(T) and CPB4-7, were isolated from marine sediments collected in Chumphon province, Thailand. Chumphon province, Thailand. Their taxonomic positions were determined using a polyphasic approach. The morphological, cultural and chemotaxonomic characteristics of these isolates were consistent with the classification of the strains as representing a member of the genus Streptomyces. They contained LL-diaminopimelic acid in their cell wall peptidoglycan; the whole-cell sugars were ribose and glucose. The predominant menaquinones were MK9-(H6) and MK9-(H8). The major polar lipids were phosphatidylethanolamine, phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides. The predominant cellular fatty acids were anteiso-C(15 : 0), iso-C(16 : 0) and iso-C(15 : 0). On the basis of 16S rRNA gene sequence similarity studies, these isolates were determined to be closely related to Streptomyces xinghaiensis JCM 16958(T) (9...