Comparative study of bioethanol production from sugarcane molasses by using Zymomonas mobilis and Saccharomyces cerevisiae (original) (raw)
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Fermentation of Sugarcane Molasses Using Zymomonas Mobilis for Enhanced Bioethanol Production
Bioethanol is one of the leading alternative biofuel to fossil fuels and can be use in existing gasoline engines without any major modification. Bioethanol can be produced from sugar containing biomass fermentation using different potential strains. In this study, the gram negative, facultative anaerobic, rod shaped strain 'Zymomonas mobilis' was used as microorganism to produce bioethanol from sugar cane molasses using anaerobic fermentation. The study was conducted to investigate the optimized conditions for production of bioethanol through batch fermentation process. The fermentation unit was designed to determine the effect of process parameters such as fermentation temperature, pH, sugar concentration and supply of nutrients. The Zymomonas mobilis produced 9.3% (v/v) bioethanol by utilizing 16 g/100mL sugar with the fermentation efficiency 92.5%. The fertilized based nutrients were supplied to enhance the production of bioethanol yield. The bioethanol yield produced by using this strain in optimized conditions is in good compromise with previous study and also compared with commercially available yeast strain.
Bioethanol production from molasses by different strains of Saccharomyces cerevisiae
In commercial ethanol production producers often use sugar cane molasses as raw material due to their abundance and low costs. The most employed microorganisms used for fermentation is Saccharomyces cerevisiae yeasts due to their ability to hydrolyze sucrose from cane molasses into glucose and fructose, two easily assimilable hexoses. The aim of this study was to evaluate the application of different strains of Saccharomyces cerevisiae for sugar cane molasses in order to produce bioethanol. According to the obtained results the strain D1 (Safdistil C-70) achieved higher values of the specific growth rate in comparison with other strains used. The maximum ethanol productivity of 2.33 g/L . h was achieved around 36 hours of fermentation by using the yeast D1. Therefore, the optimal duration of the fermentation process in technical and economic terms should be considered.
Bioethanol production from sugarcane molasses by instant dry yeast
IOP conference series, 2019
Sugarcane molasses is a by-product of sugarcane products that can be used as bioethanol production. Bioethanol is ethanol produced through a fermentation process that can be done by various microorganisms such as Saccharomyces cerevisiae. Commercial instant dry yeast on this fermentation was chosen because it can be directly used as a starter to simplify the production process and reduce the bacterial contamination risk. The sugar industry in West Java is one of the sugarcane processing industries that produce molasses in Indonesia. Then molasses were processed into bioethanol. However, the ethanol content on bioethanol production in this industry is low (± 4-6%). Some factors can cause low ethanol production such as the presence of mineral contents and sugar concentrations in molasses. The aim of this study is to investigate the effect of H 2 SO 4 pretreatment and sugar concentration on ethanol production. Three level of sugar concentration i.e. 20%, 25% and 30% Brix were compared for ethanol production using with and without pretreated molasses. The obtained data were analyzed statistically using Analysis of Variance (ANOVA). Under the best condition (30% sugar concentration with H 2 SO 4 pretreatment), the ethanol concentration could be increased by 3.5-5.5%.
45 Enhancing Bioethanol Production from Sugarcane Molasses by Saccharomyces cerevisiae Y 17
2018
Bioethanol is considered a promising type of biofuel that produced by fermentation of sugars and used as a partial gasoline replacement in different areas of the world (Sunggyu & Shah, 2012; Bhatia et al., 2012; Sadik & Halema, 2014 and Rasmey et al., 2017). The sugarcane and beet molasses are by-products of sugar industries in Egypt and are cheap raw materials, readily available, and ready for conversion with limited pretreatments as compared with starchy or cellulosic materials for bioethanol fermentation on industrial scale. Most of sugars in molasses are present in a readily fermentable form (Razmovski & Vucurovi, 2011). Sugarcane molasses is a dark viscous fluid, and rich in nutrients required by most microorganisms such as carbon, nitrogen, phosphorus, sodium, potassium and non-nitrogenous compounds. Beet molasses also is a commonly used feedstock (Dodić et al., 2009). One metric ton of sugarcane THIS STUDY aims to investigate the recycling of agro-industrial wastes as sugarca...
Production of Bio-ethanol from Sugar Molasses Using Saccharomyces Cerevisiae
Modern Applied Science, 2009
Saccharomyces cerevisiae is the cheapest strain available for the conversion of biomass substrate. In the present study, it is used for bio-ethanol production from sugar molasses. The influencing parameters that affect the production of bio-ethanol from sugar molasses are optimized. The optimal values of the parameters such as temperature, pH, substrate concentration, enzyme concentration and fermentation period are found to be 35°C, 4.0, 300 gm/l, 2 gm/l and 72 h respectively. Under this optimum operating condition the maximum of 53% bio-ethanol yield is achieved. The rate of formation of bio-ethanol is found to be well fitted with Michael-Menten equation and the rate constants such as V max and K m are found to be 0.71 mol/l sec and 81.63 mol/l respectively.
Effect of Operating Conditions on Molasses fermentation for Bioethanol production
The aim of the presented study was to evaluate the potential of molasses as bioethanol feedstock by studying the effect of different operating conditions on fermentation yield including initial sugar concentration (25-150 g/L), pH (4.5-9.5), and temperature (30-50°C). Molasses composition analyses indicated its richness with sucrose and fermentable sugars which qualify it as a promising feedstock for bioethanol production. The highest ethanol production (49 g/L) was achieved with an initial sugar = 150 g/L, pH = 4.5 but the maximum ethanol yield has been noted for: initial sugar concentration = 50 g/L, pH=4.5 and 30 °C of temperature which represent the optimum conditions for the fermentation. The kinetics study of fermentation experiment carried out under optimal conditions revealed that the fermentation reaction occurs in 3 phases: lag phase, acceleration phase and final phase.
IOP Conference Series: Earth and Environmental Science, 2018
The sugar-making industry from sugar cane has a byproduct of sugar cane as molasses. One alternative to reduce the amount of molasses waste is by reusing molasses as biomass for bioethanol production. Molasses can be reused as bioethanol that have economic value because of their high sugar content. The process of making bioethanol from molasses is done by Simultaneous Saccharification and Fermentation (SSF) method using 10% substrate (100 gr / L molasses) and 20% substrat (200 gr / L molasses). The process of hydrolysis of sugarcane molasses is carried out biologically using Aspergillus niger and Trichoderma viride, while the fermentation process uses the consortium Saccaromyces cerevisiae-Pichia stipitis. Ethanol production using the SSF methods had the highest bioethanol concentration of 14.38% v/v for 20% substrat and 10% consortium usage at 72 hours fermentation.
Ethanol production from soybean molasses by Zymomonas mobilis
Biomass and Bioenergy, 2012
This work deals with the utilization of soybean molasses (a low cost byproduct) to produce ethanol, an important biofuel, using the microorganism Zymomonas mobilis NRRL 806, a gram negative bacterium. At the first part of the work, laboratorial scale tests, using 125 mL flasks were performed to evaluate the effect of three variables on ethanol production: soybean molasses concentration (the sole carbon and nitrogen source), pH and period of previous aerobial phase. The optimal soybean concentration was around 200 g L-1 of soluble solids, pH between 6.0 and 7.0, and the period of previous aerobial phase did not provide significant effect. At the second part, kinetic tests were performed to compare the fermentation yields of Zymomonas mobilis NRRL 806 in flasks and in a bench scale batch reactor (it was obtained respectively 78.3% and 96.0% of the maximum theoretical yields, with productions of 24.2 and 29.3 g L-1 of ethanol). The process with a reactor fermentation using Saccharomyces cerevisiae LPB1 was also tested (it was reached 89.3% of the theoretical maximum value). A detailed kinetic behavior of the molasses sugars metabolism for Z. mobilis was also shown, either in reactor or in flasks. This work is a valuable tool for further works in the subject of ethanol production from agro-industrial by-products.
Microbiology and Biotechnology Letters
Bioethanol has recently attracted much attention as a sustainable and environmentally friendly alternative energy source. This study aimed to develop a potential process for bioethanol production by fed-batch fermentation using instant dry yeast. To obtain the highest cell growth, we studied the influence of the initial sugar concentrations and pH of sugarcane molasses in batch fermentation. The batch system employed three levels of sugar concentrations, viz. 10%, 15%, 20% (w/v), and two levels of pH, 5.0 and 5.5. The highest cell growth was achieved at 20% (w/v) and pH 5.5 of molasses. The fed-batch system was then performed using the best batch fermentation conditions, with a molasses concentration of 13% (w/v) which resulted in high ethanol concentration and fermentation efficiency of 15.96% and 89%, respectively.
Bioresource Technology, 2008
The aim of this work was to develop an economical bioprocess to produce the bio-ethanol from soybean molasses at laboratory, pilot and industrial scales. A strain of Saccharomyces cerevisiae (LPB-SC) was selected and fermentation conditions were defined at the laboratory scale, which included the medium with soluble solids concentration of 30% (w/v), without pH adjustment or supplementation with the mineral sources. The kinetic parameters -ethanol productivity of 8.08 g/L h, Y P/S 45.4%, Y X/S 0.815%, m 0.27 h À1 and l X 0.0189 h À1 -were determined in a bench scale bioreactor. Ethanol production yields after the scale-up were satisfactory, with small decreases from 169.8 L at the laboratory scale to 163.6 and 162.7 L of absolute ethanol per ton of dry molasses, obtained at pilot and industrial scales, respectively.