Bioethanol Production from Sugarcane Molasses by Fed-Batch Fermentation Systems Using Instant Dry Yeast (original) (raw)
Related papers
Bioethanol production from sugarcane molasses by instant dry yeast
IOP conference series, 2019
Sugarcane molasses is a by-product of sugarcane products that can be used as bioethanol production. Bioethanol is ethanol produced through a fermentation process that can be done by various microorganisms such as Saccharomyces cerevisiae. Commercial instant dry yeast on this fermentation was chosen because it can be directly used as a starter to simplify the production process and reduce the bacterial contamination risk. The sugar industry in West Java is one of the sugarcane processing industries that produce molasses in Indonesia. Then molasses were processed into bioethanol. However, the ethanol content on bioethanol production in this industry is low (± 4-6%). Some factors can cause low ethanol production such as the presence of mineral contents and sugar concentrations in molasses. The aim of this study is to investigate the effect of H 2 SO 4 pretreatment and sugar concentration on ethanol production. Three level of sugar concentration i.e. 20%, 25% and 30% Brix were compared for ethanol production using with and without pretreated molasses. The obtained data were analyzed statistically using Analysis of Variance (ANOVA). Under the best condition (30% sugar concentration with H 2 SO 4 pretreatment), the ethanol concentration could be increased by 3.5-5.5%.
IOP Conference Series: Earth and Environmental Science
Some bioethanol industries in Indonesia that use sugarcane molasses as raw material often face low level of ethanol content from fermentation process. The molasses condition and the temperature fluctuation are supposed to be the cause. This research aimed to determine the effect of sugarcane molasses pre-treatment with H 2 SO 4 and fermentation temperature in bioethanol production by using instant dry yeast of Saccharomyces cerevisiae. Factorial Randomized Block Design with 2 factors was used in the research. The first factor was pre-treatment effect in sugarcane molasses (with and without pre-treatment) and the second was fermentation temperature (29 0 C, 32 0 C and 35 0 C). The data were analysed using analysis of variance or ANOVA and continued with further test Duncan Multiple Range Test or HSD (Honestly Significant Difference) with confidence interval of 5%. The best treatment was determined using Multiple Attribute Test. Results showed that sugarcane molasses pretreatment with H 2 SO 4 and fermentation temperature had a significant effect (α= 0.05) on bioethanol production. The best result was obtained from sugarcane molasses medium that given H 2 SO 4 pre-treatment and fermentation temperature of 32 0 C with total decrease value of total soluble solids (% brix) of 10.9 % brix, total decrease sugar of 12.15%, reducing sugar consumption of 57.21 g/L, ethanol content of 8.30 % and yield ethanol of 68.67 %.
45 Enhancing Bioethanol Production from Sugarcane Molasses by Saccharomyces cerevisiae Y 17
2018
Bioethanol is considered a promising type of biofuel that produced by fermentation of sugars and used as a partial gasoline replacement in different areas of the world (Sunggyu & Shah, 2012; Bhatia et al., 2012; Sadik & Halema, 2014 and Rasmey et al., 2017). The sugarcane and beet molasses are by-products of sugar industries in Egypt and are cheap raw materials, readily available, and ready for conversion with limited pretreatments as compared with starchy or cellulosic materials for bioethanol fermentation on industrial scale. Most of sugars in molasses are present in a readily fermentable form (Razmovski & Vucurovi, 2011). Sugarcane molasses is a dark viscous fluid, and rich in nutrients required by most microorganisms such as carbon, nitrogen, phosphorus, sodium, potassium and non-nitrogenous compounds. Beet molasses also is a commonly used feedstock (Dodić et al., 2009). One metric ton of sugarcane THIS STUDY aims to investigate the recycling of agro-industrial wastes as sugarca...
IOP Conference Series: Earth and Environmental Science, 2018
The sugar-making industry from sugar cane has a byproduct of sugar cane as molasses. One alternative to reduce the amount of molasses waste is by reusing molasses as biomass for bioethanol production. Molasses can be reused as bioethanol that have economic value because of their high sugar content. The process of making bioethanol from molasses is done by Simultaneous Saccharification and Fermentation (SSF) method using 10% substrate (100 gr / L molasses) and 20% substrat (200 gr / L molasses). The process of hydrolysis of sugarcane molasses is carried out biologically using Aspergillus niger and Trichoderma viride, while the fermentation process uses the consortium Saccaromyces cerevisiae-Pichia stipitis. Ethanol production using the SSF methods had the highest bioethanol concentration of 14.38% v/v for 20% substrat and 10% consortium usage at 72 hours fermentation.
Bioethanol production from molasses by different strains of Saccharomyces cerevisiae
In commercial ethanol production producers often use sugar cane molasses as raw material due to their abundance and low costs. The most employed microorganisms used for fermentation is Saccharomyces cerevisiae yeasts due to their ability to hydrolyze sucrose from cane molasses into glucose and fructose, two easily assimilable hexoses. The aim of this study was to evaluate the application of different strains of Saccharomyces cerevisiae for sugar cane molasses in order to produce bioethanol. According to the obtained results the strain D1 (Safdistil C-70) achieved higher values of the specific growth rate in comparison with other strains used. The maximum ethanol productivity of 2.33 g/L . h was achieved around 36 hours of fermentation by using the yeast D1. Therefore, the optimal duration of the fermentation process in technical and economic terms should be considered.
Enhancing Bioethanol Productivity from Sugarcane Molasses by Saccharomyces cerevisiae Y17 KP096551
Egyptian Journal of Botany
is capable of producing approximately 106kg of sugar and 46kg of molasses. A typical molasses to ethanol conversion rate is 4kg of molasses/L ethanol (Lavarack, 2001). However, this rate can vary based on production practices and sugar content of the molasses (Nguyen et al., 2009 and Silalertruksa & Gheewala, 2010). Molasses composition is usually influenced by the variety and maturity of the cane and beet, soil, climate and the processing conditions in the factory (El-Gendy et al., 2013). High yielding and efficient fermentation of molasses with varying composition requires selection of special yeast strains having high tolerances to inhibitory conditions as well as ability of fast fermentation. Other natural requirements like tolerance to high alcohol, sugar and temperature are also necessary (Bazmi et al., 2007
2014
Fresh and modified sugarcane bagasse as a carrier of immobilized cells for ethanol production were investigated. Modified sugarcane bagasse was obtained from serial treatments respectively namely steam only, pressure with steam, and by combining both of steam procedure. Aside from that, biocatalyst 1% (w/v) (fresh and modified carrier that containing cells) were used as an inoculum for ethanol fermentation. The best modified sugarcane bagasse for carrier of immobilized cells was obtained using steam treatment for 30 minutes by improving the physical properties of carrier so that increasing the ethanol yield. A maximum ethanol yield was 0.40 0.01 g/g in 24-hour of fermentation period. This maximum ethanol production showed 1.6 and 6 times higher result compare to immobilized cells on fresh sugarcane bagasse and free cells system, respectively.
The study was designed to compare the Bioethanol production from Zymomonas mobilis and Saccharomyces cerevisiae using molasses as production medium. The focus was on the retention time at lab scale. Bioethanol and petroleum blend can be used in existing gasoline engines. Present study showed a more cost-effective procedure for production of ethanol from sugar-cane molasses by using bacterial strain "Z. mobilis". Laboratory scale unit was designed to perform the experiments through batch fermentation and to determine the impact of leading parameters, including fermentation temperature, pH, sugar concentration, and nutrients. S. cerevisiae produced 8.3% (v/v) bioethanol provided sugar concentration 14 g /100 ml with the fermentation efficiency of 92.5%. On the contrary, Z. mobilis produced 9.3% (v/v) bioethanol by utilizing 16 g/100 ml sugar with the fermentation efficiency of 90.5%. Effect of nutrients on fermentation was determined using molasses as feedstock. Thin layer chromatography was also performed to assess the possible impurities in molasses as compared to the pure sugar. The pH and fermentation temperature was optimized for the enhanced yield of bioethanol.
Bioethanol Production from Fermentable Sugar Juice
The Scientific World Journal
Bioethanol production from renewable sources to be used in transportation is now an increasing demand worldwide due to continuous depletion of fossil fuels, economic and political crises, and growing concern on environmental safety. Mainly, three types of raw materials, that is, sugar juice, starchy crops, and lignocellulosic materials, are being used for this purpose. This paper will investigate ethanol production from free sugar containing juices obtained from some energy crops such as sugarcane, sugar beet, and sweet sorghum that are the most attractive choice because of their cost-effectiveness and feasibility to use. Three types of fermentation process (batch, fed-batch, and continuous) are employed in ethanol production from these sugar juices. The most common microorganism used in fermentation from its history is the yeast, especially, Saccharomyces cerevisiae, though the bacterial species Zymomonas mobilis is also potentially used nowadays for this purpose. A number of factors related to the fermentation greatly influences the process and their optimization is the key point for efficient ethanol production from these feedstocks.
Effect of Operating Conditions on Molasses fermentation for Bioethanol production
The aim of the presented study was to evaluate the potential of molasses as bioethanol feedstock by studying the effect of different operating conditions on fermentation yield including initial sugar concentration (25-150 g/L), pH (4.5-9.5), and temperature (30-50°C). Molasses composition analyses indicated its richness with sucrose and fermentable sugars which qualify it as a promising feedstock for bioethanol production. The highest ethanol production (49 g/L) was achieved with an initial sugar = 150 g/L, pH = 4.5 but the maximum ethanol yield has been noted for: initial sugar concentration = 50 g/L, pH=4.5 and 30 °C of temperature which represent the optimum conditions for the fermentation. The kinetics study of fermentation experiment carried out under optimal conditions revealed that the fermentation reaction occurs in 3 phases: lag phase, acceleration phase and final phase.