Dose dependence specific and non-specific immune responses of Indian major carp (L. rohita Ham) to intraperitoneal injection of formalin killed Aeromonas hydrophila whole cell vaccine (original) (raw)
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Veterinary Research …, 2011
Specific and non-specific immune response to different doses of formalin killed whole cell vaccine of Aeromonas hydrophila to Indian major carp (Labeo rohita) was evaluated in laboratory condition. Three different doses (10 5 CFU/ml, 10 7 CFU/ml, 10 10 CFU/ml) were administered (0.2 ml/fish) intraperitoneally for 1 month. Among the three doses, 10 10 CFU/ml elicited the highest antibody and protective response followed by the doses 10 7 CFU/ml and 10 5 CFU/ml. Upon challenge with the virulent strain of A. hydrophila, the relative percentage of survival was recorded up to 80% at highest dose of 10 10 CFU/ml. The non-specific responses, similar to the specific immune responses were also maximum at highest dose of 10 10 CFU/ml. Similar to the specific immune responses, the non-specific responses were maximum at highest dose of 10 10 CFU/ml. Therefore, dose containing 10 10 CFU/ml of formalin killed cells was found to be the most effective dose for vaccination which increased the immunity in Indian major carp (Labeo rohita) to a larger extent.
Iranian Journal of Fisheries Sciences, 2019
The success of many vaccines relies on their association with selected adjuvants in order to increase their immunogenicity and ensure long-term protection. Propolis is a natural compounds, are mostly known for their immunostimulatory properties. In this study the adjuvant effects of propolis in combination with formalin-killed A. hydrophila vaccine in Common carp (Cyprinus carpio) were evaluated and compared with Freund’s adjuvant. Three hundred juvenile carp (weighing 50.4 ± 3.2 g) were divided randomly into four groups in triplicates. The fish were intraperitonealy injected with Aeromonas hydrophila bacterin (A.h) in combination with normal saline (Formalin Killed Bacterin, FKB group), Freound (FKB +F group), and Propolis (FKB +P group) respectively. Control group were injected with normal saline. Serum samples were taken from fish in each group every other weeks (days 0, 14, 28 and 42) of experiment and immunological parameters including anti A.h antibody titer, serum lysozym...
IOP Conference Series: Earth and Environmental Science, 2020
The study aim was to find the effect of different vaccination application methods on hematological parameters of catfish (Clarias sp). A 107 Aeromonas hydrophila measured by 625 nm wave length of Spectrophotometer was applied on 25 catfish with average weight 15.83 g. The application methods were dipping (1 ml/10 l water) and intramuscular injection (0.1 ml/fish). Hematological parameter measured were hematocrit levels, total leukocytes and phagocytic index. Measurement were conduct on day 0, 21, 23 and 27 after vaccine application. The results showed that hematocrit level on fish with injection method were higher (21.02%) than dipping method (15,05%), total leukocytes on fish with dipping method were higher (3,29 x 104 cells/mm3) than injection method (3,15 x 104 cells/mm3) and phagocytic index on fish with injection were higher (25%) than dipping method (23%). The survival of catfish were also observed, and showed that catfish with injection method have higher survival rate (36%) ...
Veterinary Immunology and Immunopathology, 2007
The immune response to mixed whole cell antigens of Aeromonas hydrophila, Edwardsiella tarda and Pseudomonas fluorescens, the common Gram negative bacterial pathogens associated with diseases of Indian major carps were evaluated for their efficacy in triggering antibody responses in rohu, Labeo rohita (Ham.). The rohu yearlings were either immunized with antigens from single bacterial strain, A. hydrophila, E. tarda and P. fluorescens or a combination of all three. An antibody response was detected at 1st week post immunization that rose significantly (p<0.05) at 4th week post immunization in all the immunized groups. The antibody level started declining after 8th week but persisted up to 10th week post immunization in all the immunized groups. Similarly, no significant difference (p>0.05) in the antibody level was found between groups immunized with single and mixed bacterial antigens. Moreover, the use of mixed bacterial antigens did not jeopardize the specific immune response to the vaccine components. Upon challenge with single pathogen, a high relative percent survival was recorded in the group immunized with mixed bacterial antigens and was comparable to those fish immunized with the single bacteria.
Advances in Bioscience …, 2010
The effects of injectable killed Aeromonas hydrophila on lymphocyte populations and on serum proteins of juvenile common carp, Cyprinus carpio, were examined. The fishes were injected either intraperitoneally or intramuscularly with killed A. hydrophila bacterin isolated from a diseased fish. 15 days after injection the lymphocyte count was significantly higher in the kidney of intraperitoneally bacteria injected fishes than in the normal saline injected fishes (p < 0.05), but in the blood smears the lymphocyte count difference was not significant (p > 0.05). In the intramuscular bacteria injected fishes in comparison with the normal saline group, the lymphocyte count was significantly higher only in the blood (p < 0.05) but in the kidney the difference was not significant (p > 0.05). In comparison between the serum protein parameters in different groups, in intraperitoneal injection of killed-bacteria, total albumin as well as the ratio of albumin/globulin was greater than the control group (p < 0.05). The other fractions had not showed any significant difference (p > 0.05). In intramuscular injection, the normal saline-injected group, amount of α-globulin was significantly higher than bacteria-injected group (p < 0.05). For the other fractions there was no significant difference between two groups (p > 0.05).
International Journal of Zoological Investigations, 2023
The experimental fish Channa striatus was divided as control and experimental groups. To find out the efficacy of Aeromonas hydrophila vaccine, the fish were infected with Gram negative Bacterial Strain Aeromonas hydrophila with a dosage of 0.1 ml. The samples of blood were collected on the 1 st , 3 rd , 5 th , 7 th and 14 th day intervals. The serum was evaluated for the biochemical parameters such as Protein, Albumin and Globulin, ratio of Albumin and Globulin and blood glucose level. SDS PAGE analysis was made to find out different protein fractions in the blood sample of fish which was treated earlier with Aeromonas hydrophila. Another set of fish was treated with heat killed and formalin (chemically) killed attenuated vaccine of A. hydrophila. Then the vaccinated fish was post challenged by infected with A.hydrophila to find out the efficacy of the vaccine. The results were tabulated and statistically analyzed. The present investigation revealed that the administration of vaccines could enhance the fish defense mechanisms. However, the heat killed (HK) vaccine showed great impact on improving the health condition of fish by enhancing its resistance capacity against pathogenic bacteria.
Diseases of aquatic …, 2000
Uptake and processing of biofilm (BF) and free-cell (FC) vaccines of Aeromonas hydrophila were studied in the Indian major carps catla Catla catla, and rohu Labeo rohita and in the common carp Cyprinus carpio following a single dose oral vaccination of 10 11 CFU g -1 fish. Fish were sampled at 0.5, 1, 3, 6, 12, 24 h and at 2, 3, 5 and 10 d following vaccination and antigen localization was studied in the gut, kidney and spleen employing monoclonal antibody based immunofluorescence and immunoperoxidase. The general pattern of antigen localization was similar in catla, rohu and common carp. Initially, both the BF and FC antigens were localized in the gut lumen, followed by their uptake by intraepithelial vacuoles and macrophages. Antigen administered orally was also seen in the spleen and kidney. Both BF and FC antigens were detected in the gut lumen of carp within 30 min following oral delivery. However, BF antigen remained in the lumen of the hindgut for 48 h compared to 6 h in the case of FC antigen. In the early stages, BF antigen was localized in the gut epithelial vacuoles while FC antigen was associated with the small macrophages of the hindgut. Antigen localization in spleen and kidney was observed at 3 h and persisted even up to 10 d following oral delivery. In general, there was a distinct difference between BF and FC vaccines in the duration of retention and quantity of uptake in the gut, kidney and spleen.
Journal of Entomology and Zoology Studies, 2020
To develop vaccine for rohu (Labeo rohita), efficacy of three antigenic preparations from Aeromonas hydrophila were evaluated. Thirty six tanks with ten rohu were divided in quadruplicates (R1 to R4) with nine tanks (G1 to G9). Rohu of G1 to G6 tanks were given intraperitoneal vaccine with outer membrane protein, somatic protein and formalin-inactivated whole cell itself and along with Incomplete Freund’s Adjuvant @ 200 µg/fish, G7 and G8 tanks were injected with Incomplete Freund’s Adjuvant (100 µl/fish) and normal saline (100 µl/fish) respectively and G9 tanks were kept as control. After 28 d, rohu of R3 and R4 were subjected to intramuscular A. hydrophila challenge (LD50) @ 2.85×106 cells/fish for 7 d and RPS (%) was calculated. Specific cellular and humoral immune responses were determined for rohu of R1 and R2. Results showed that rohu immunized with outer membrane protein along with adjuvant could offer an appropriate vaccine strategy.