Phytochemical, antimicrobial and cytotoxic evaluation of Indigofera serpentinicola (original) (raw)
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Journal of Enzyme Inhibition and Medicinal Chemistry, 2009
The antibacterial, antifungal, acute cytotoxicity, phytotoxicity and insecticidal profile of the crude extract and various fractions of Indigofera gerardiana have been studied. Six bacterial and fungal strains were used, of which Samonella typhi and Microsporum canis were the most susceptible strains with MICs 0.37 mg/mL and 0.09 mg/mL, respectively. The crude extract and the fractions showed low insecticidal activity against Sitophilus oryzae, Rhyzopertha dominica and Callosbruchus analis but no activity against Tribolium castaneum. The brine shrimp lethality assay showed absence of any measurable cytotoxicity of the crude extract and fractions, showing a good safety profile at a preliminary level. All the fractions except crude extract revealed profound and highly significant herbicidal activity against Lemna minor at the concentration of 1000 mg/mL. Indigofera gerardiana was shown by in-vitro assays to be a potential source for natural antifungal, antibacterial and herbicidal agents.
Antimicrobial efficacy and phytochemical analysis of Indigofera trita Linn
African Journal of Traditional, Complementary and Alternative Medicines, 2013
An in vitro antimicrobial activity and phytochemical analysis of various extracts of Indigofera trita L. viz. petroleum ether, chloroform, acetone, ethanol and aqueous extracts were carried out. A total of 21 microorganisms (19 bacteria and 2 fungal strains) were used for antimicrobial activity by disc diffusion method and a standard procedure was used to identify the phytochemical constituents. Petroleum ether extract showed moderate inhibitory activity against Staphylococcus aureus (14.40 mm),
Antimicrobial efficacy and phytochemical analysis of Indigofera trita Linn
African Journal of Traditional, Complementary and Alternative Medicines, 2013
An in vitro antimicrobial activity and phytochemical analysis of various extracts of Indigofera trita L. viz. petroleum ether, chloroform, acetone, ethanol and aqueous extracts were carried out. A total of 21 microorganisms (19 bacteria and 2 fungal strains) were used for antimicrobial activity by disc diffusion method and a standard procedure was used to identify the phytochemical constituents. Petroleum ether extract showed moderate inhibitory activity against Staphylococcus aureus (14.40 mm),
Phytochemical and cytotoxicity testing of Indigofera lupatana Baker F.
Indigenous plants are promising as a cheap and alternative complimentary medicine (CAM). Although the use of phytomedicine has been in practice for long, little has been done to evaluate their effectiveness, safety, target organisms and also their chemical characterization. Indigofera lupatanaBaker F. (locally known as Mugiti) has been used by Mbeere community of Kenya to treat many conditions suchas cough, diarrhea, pleurisy and gonorrhea. In this study, powdered sample of I. lupatanaBaker F. roots were sequentially extracted using hexane, ethyl acetate, dichloromethane and methanol solvents. The resultant extract fractions were subjected to phytochemical tests and brine shrimp cytotoxicity bioassay. Results showed that the fractions had Phenolics, Flavonoids, Tannins, Saponins, Terpenoids, Cardiac glycosides, Steroids and Phlobatannins. These are responsible for the bioactivity of the sample fractions.All fractions had a LC50value greater than 1000µg/ml which is an indication that they are all non toxic. Therefore Indigofera lupatanaBaker F. can be used as an alternative source of new, effective, safe drug(s) because it demonstrated presence of phytochemicals with no oral toxicity.
Phytochemical and Biological screening of the seeds of Indigofera heterantha wall
Indigofera heterantha seeds are used in folk medicine for the treatment of gastrointestinal disorder and abdominal pain. Phytochemical and biological Screening on the seeds extracts were carried out. Alkaloids, steroids, terpenoids, flavonoids, anthraquinones, tannins, phlobatanins, saponins, glycosides and reducing sugars were detected in the extract. The antibacterial activity tested against Escherichia coli, Bacillus Subtilis, Shigella flexenari, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella typhi were negative. The Brine shrimp and insecticidal activity against Tribolium castaneum, Rhyzopertha dominica and Callosbruchuanalis were found negative at the concentration of 1019.10 µg/cm .
Antimicrobial, insecticidal and phytotoxic activities of Indigofera heterantha roots
Indigofera heterantha is a medicinal plant available in northern areas of Pakistan. Locally used for various diseases. In this study, we have made an effort to investigate various activities to scientifically validate some of its pharmacological activities of its root extract and fractions. Among in vitro assays no antibacterial activity was found while low antifungal activity was observed. Similarly, low insecticidal and low to moderate phytotoxicity was identified in case of fractions of I. heterantha.
phytochemical invitigation of seeds of indigofera heterantha
Indigofera heterantha seeds are used in folk medicine for the treatment of gastrointestinal disorder and abdominal pain. Phytochemical and biological Screening on the seeds extracts were carried out. Alkaloids, steroids, terpenoids, flavonoids, anthraquinones, tannins, phlobatanins, saponins, glycosides and reducing sugars were detected in the extract. The antibacterial activity tested against Escherichia coli, Bacillus Subtilis, Shigella flexenari, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella typhi were negative. The Brine shrimp and insecticidal activity against Tribolium castaneum, Rhyzopertha dominica and Callosbruchuanalis were found negative at the concentration of 1019.10 µg/cm . 2
ANTIBACTERIAL ACTIVITY OF CRUDE EXTRACTS OF INDIGOFERA ASPALATHOIDES VAHL. EX. DC.
Plants are rich in a wide variety of secondary metabolites such as tannins, terpenoids, alkaloids and flavonoids which have been found in-vitro to have antimicrobial properties. The present study was conducted with a view to evaluate the therapeutic potentials Indigofera aspalathoides plant extracts against human pathogen. The leaves and aerial parts of Indigofera aspalathoides were dried and powdered leaf and stem material was extracted by soxhlation method using a non-polar (Hexane) solvent and polar solvents (Chloroform, Ethanol and Water), respectively and extracts were then subjected to phytochemical screening. Extract of Indigofera aspalathoides was evaluated for its antimicrobial activity against a wide variety of pathogenic bacteria such Escherichia coli, Klebsiella pneumonia and Aeromonas hydrophila by disc diffusion method. In the extract of I.aspalathoides maximum yield was obtained from in the leaf ethanol (20.64±1.02) than the chloroform (15.80±3.28) and hexane (6.52±1.78) and compare the stem extracts ethanol(10.23±3.55) was in high followed by chloroform(7.28±2.36) and hexane (3.52±1.35). Phytochemical analysis showed the presence of various phytoconstituents. Alkaloids, Phenolic compounds, Flavonoids, Tannins and Terpenoids were found to be present in all extracts of leaf and stem. Reducing sugar was absent both leaf and stem. Pholobatanins recorded in leaf and absent were in stem.Compare the solvent extracts of leaf, crude ethanol extracts of I. aspalathoides leaf caused more antibacterial activity against pathogens E. coli (24.4±2.5), B. subtilis (23.3±2.4) and A. hydrophila (20.6±3.1) followed by chloroform against E. coli (18.2±1.5) and hexane against A. hydrophila (11.2±1.2). Compare the stem extracts; hexane extracts caused more antibacterial activity against K. pneumonia (29.2±2.9) followed by chloroform against B. subtilis (25.2±0.4) and against B. subtilis (12.7±0.8). The results support that these plant extracts can be used for the treatment of bacterial diseases.
International journal of Pharmacy and Pharmaceutical Sciences, 2014
Objective: The current study was done to analyze qualitatively the presence of pharmacological compounds and the antibacterial activity of different extracts of Indigo feraarrecta. Methods: The plant was extracted using hydromethanolic solvent. The extracted compounds were dissolved in different solvents according to their polarity and then concentrated using a rotar vapor with a water bath at 40 o C. The antibacterial assessment was done using well diffusion method and the phytochemicals analysed using standard procedures with minor adjustments. Results: The study demonstrated thatthe plant contains very important pharmacological compounds. The extracts tested had different inhibitions on the microorganisms tested. Ethyl acetate extract inhibited the growth of all the selected pathogenic organisms while the water extract inhibited the growth of three out of the five microorganisms tested. Hexane and butanol inhibited only Bacillus cereus out of the five microorganisms used in the study. The penicillin which was used as the positive control inhibited the growth of all the organisms, while dimethylsulfoxide(DMSO) did not inhibit the growth of any of the organisms.. One-way analysis of variance (ANOVA) showed there was significant difference in the microorganisms zones of inhibition (P<0.05). Conclusion: The results obtained in this research are a scientific justification of the plant's use in the treatment of various diseases. The results have revealed thatthe plant has great potency in the treatment against infections caused by all the bacteria tested. However, further research needs to be done to isolate the active compounds, identify their structural formula, their mode of action and their effect in the in vivo environment.