Mycoplasmas associated with canine infectious respiratory disease (original) (raw)
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Isolation and Identification of Mycoplasma Species in Dogs
Agriculture Journal IJOEAR, 2020
Mycoplasmas can be associated with several canine health issues, mainly when dogs do not respond to antimicrobial treatment usually aimed at bacterial infections. Different mycoplasma species can be found in both healthy and sick animals; however, the following subjects have yet to be fully understood: The role played by mycoplasmas in canine habitats and the various diseases caused by them. The aim of the present study is to assess the presence of mycoplasma in dog samples at NUDMIC/UFF, RJ, Brazil, over a timeframe of ten years. Out of all assessed dogs, 9.67% (15/155) had respiratory symptoms, whereas the rest of them were asymptomatic. Moreover, 29.96% of the cultured samples (77/257) were positive for mycoplasmas. Typical colonies of said samples were divided into 42.86% (33/77) of oropharynx samples, 51.95% (40/77) of urogenital samples and 5.19% (4/77) of samples from other sources. Species Mycoplasma canis, Mycoplasma edwardii and Mycoplasma cynos were identified by PCR and/or immunoperoxidase. The most common species was M. canis. M. cynos was found in a dog with signs of respiratory disease. Despite the recent improvement in early identification and the biomolecular knowledge surrounding canine mycoplasma, the etiopathogenesis of canine mycoplasmosis remains uncertain..
Strain typing of Mycoplasma cynos isolates from dogs with respiratory disease
Veterinary Microbiology, 2009
The association of Mycoplasma cynos with canine infectious respiratory disease is increasingly being recognised. This study describes the strain typing of 14 M. cynos isolates cultured from trachea and bronchoalveolar lavage samples of six dogs with respiratory disease, from two separate kennels in the United Kingdom. The genetic similarity of the isolates was investigated using pulsed-field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD). Most of the isolates from four dogs housed at a re-homing kennel were genetically similar and some isolates Manuscript Page 2 from different dogs were indistinguishable by both PFGE and RAPD. These isolates were cultured from dogs with non-overlapping stays in the kennel, which may indicate maintenance of some strains within kennels. A small number of isolates showed much greater genetic heterogeneity and were genetically distinct from the main group of M. cynos strains. There was also a high degree of similarity of the M. cynos type strain (isolated from a dog with respiratory disease in Denmark in 1971) to at least one of the United Kingdom isolates using PFGE analysis, which may suggest possible conservation of pathogenic strains of M. cynos.
This review aims to summarise our current understanding of the role of mycoplasmas in domestic dogs. Canine mycoplasmology is a small field, with less than 50 publications in the past 40 years. In this time we have gained knowledge about the number of species and have made associations with infections in dogs. However much evidence is still lacking. The importance of all canine mycoplasmas remains unknown, yet certain species are associated with canine anaemia (Mycoplasma haemocanis), respiratory disease (Mycoplasma cynos) and urogenital tract infections (Mycoplasma canis). Mycoplasmas can be isolated in pure culture from canine clinical specimens and it is hoped that this review will stimulate veterinarians to consider mycoplasmas as a potential cause of disease in dogs, especially when antibiotic therapy is failing.
Isolation of Mycoplasma species from respiratory infections of sheep and goats
The present study is carried out on isolation of Mycoplasma species from respiratory infections of sheep and Goats because Mycoplasma is also one of the major etiological agent in the production of respiratory infections which produces economic losses to small-scale farmers. In the present study a total of 188 Mycoplasma suspected samples, 95 (44 nasal swabs, 49 lung tissues and 2 pleural fluids) from sheep and 93 (56 nasal swabs, 33 lung tissues and 4 pleural fluids) from goats were collected. Isolation carried out on PPLO broth and PPLO agar. Positive samples produce color change in broth from brown to yellow and production of two different types of colonies on agar. One type is typical fried egg colony and other type is nipple-like with prominent tip. Conformation also done by PCR targeting Mycoplasma genus yielding 280 bp product size.
Tropical animal health and production, 2013
This study used cultures, polymerase chain reaction (PCR), and immunoperoxidase to examine samples from 216 lungs from sheep and lambs with macroscopic pneumonia lesions for the presence of Mycoplasma species. DNA was extracted from lung tissue samples and broth cultures with the help of a DNA extraction kit and replicated using genus-specific and species-specific primers for mycoplasma. The lung samples were examined by the immunoperoxidase method using hyperimmune Mycoplasma ovipneumoniae serum. The randomly amplified polymorphic DNA (RAPD) test was used for the molecular typing of M. ovipneumoniae isolates. Mycoplasma was isolated in the cultures of 80 (37.03 %) of a total of 216 lung samples. Genus-specific mycoplasma DNA was identified by PCR in 96 (44.44 %) samples in broth cultures and 36 (16.66 %) directly in the lung tissue. Of these 96 cases in which genus-specific identification was made, 57 (59.37 %) were positive for reaction with species-specific primers for M. ovipneu...
Assiut Veterinary Medical Journal, 2018
The purpose of this study was to evaluate the role and variety of Mycoplasma arginini (M. arginini) in respiratory manifestation in sheep and goat flocks. M. arginine, were isolated from 40 nasal swabs out of 160 nasal swabs examined which obtained from (79) sheep and (81) goat with respiratory manifestations. In sheep, the percent of isolation was (25.32%), 20 isolate out of 79 nasal swabs, while the percent of isolation in goat was (24.69%) 20 isolate out of 81 nasal swabs examined. Higher percent of isolation was found in age group (1-2) year old sheep (50%) as 7 isolates out of 14 nasal swabs examined, while in goat the higher percent of isolation was in age group less than one year by 44% (11 isolates out of 25 nasal swabs examined. The lower isolation rates were found in adult sheep and goat (3-4 years old) by 15.38% in sheep and 6.45% in goat. Mycoplasma isolates was molecularly characterized by PCR and DNA sequencing as M. arginini and submitted to Gen Bank. An accession number has been assigned to each nucleotide sequence and was KP972458-KP972459.
2012
using a polymerase chain reaction assay species in cats with lower airway disease: improved detection and species identification Mycoplasma technique does not amount to an endorsement of its value or quality, or the claims made by its manufacturer. those of the authors and the inclusion in this publication of material relating to a particular product, method or of animals and interpretation of published materials lies with the veterinary practitioner. The opinions expressed are from actions or decisions based on information contained in this publication; ultimate responsibility for the treatment arising country. The authors, editors, owners and publishers do not accept any responsibility for any loss or damage advertising material, it is the responsibility of the reader to check that the product is authorised for use in their own bear this in mind and be aware of the prescribing laws pertaining to their own country. Likewise, in relation to Furthermore, drugs may be mentioned that are licensed for human use, and not for veterinary use. Readers need to formulations that are not available or licensed in the individual reader's own country.
Mycoplasma canis and urogenital disease in dogs in Norway
The Veterinary record, 2003
Mycoplasmas identified as Mycoplasma canis were isolated from nine dogs with clinical signs of urogenital disease in Norway over a period of 20 months. Some of the dogs had been treated unsuccessfully with antibiotics, and three were euthanased as a result of severe persistent disease. Seven of the dogs had a urinary tract infection, one had chronic purulent epididymitis and one had chronic prostatitis. Overt haematuria was frequently observed among the dogs with cystitis. M canis was isolated in pure culture from seven of the dogs and in mixed culture from the other two. In three cases the mycoplasma was cultivated only from urinary sediment, and it was typically obtained in smaller numbers than would be considered indicative of a urinary tract infection. In contrast with most mycoplasmas, the M canis isolated from all the dogs grew on ordinary blood agar plates used for routine bacteriological cultivation. Specific mycoplasma media were not used and the presence of other Mycoplasm...
Pharma innovation, 2023
Infections of respiratory tract were one of the main problems in sheep and goats farming. Causing production and economic losses to farmers. Mycoplasma is one of etiological agent. Among all species, not only Mycoplasma mycoides cluster group but also non cluster organisms play important role in production of disease. In the present study a total of 188 Mycoplasma suspected samples, 95 (44 nasal swabs, 49 lung tissues and 2 pleural fluids) from sheep and 93 (56 nasal swabs, 33 lung tissues and 4 pleural fluids) from goats were collected. Molecular detection of genus Mycoplasma, Mycoplasma ovipneumoniae and Mycoplasma agalactiae was carried out by PCR targeting 16SrRNA yielding 280 bp, 361 bp and 360 bp product. The overall incidence of genus Mycoplasma was found to be 68.42% in sheep and 55.91% in goats. The overall incidence of Mycoplasma ovipneumoniae and Mycoplasma agalactiae was found to be 32.63% & 12.63% in sheep and17.2% &13.97% in goats.