Epitope mapping (original) (raw)
تخطيط محدد مولد الضد (بالإنجليزية: Epitop mapping) هو عملية يتم فيها تحديد مواقع الارتباط أو «التكوينات الفوقية (الإبيتوبات)» للأجسام مضادة على مولدات الضد المستهدفة والتي تكون عادة بروتينات.
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dbo:abstract | تخطيط محدد مولد الضد (بالإنجليزية: Epitop mapping) هو عملية يتم فيها تحديد مواقع الارتباط أو «التكوينات الفوقية (الإبيتوبات)» للأجسام مضادة على مولدات الضد المستهدفة والتي تكون عادة بروتينات. (ar) Die Epitopkartierung (engl. epitope mapping) beschreibt die Bestimmung von Epitopen auf Antigenen. Darunter befinden sich sowohl MHCI-gebundene Epitope, die durch den T-Zell-Rezeptor auf cytotoxischen T-Zellen erkannt werden, als auch MHCII-gebundene Epitope, die durch CD4-positive T-Zellen erkannt werden sowie lösliche oder Zellmembran-gebundene Antigene, deren Epitope durch Antikörper von B-Zellen erkannt werden. Die Epitopkartierung dient der Identifikation von immundominanten Epitopen für potentielle Impfstoffe, sowie der Entwicklung therapeutischer und diagnostischer Präparate. (de) In immunology, epitope mapping is the process of experimentally identifying the binding site, or epitope, of an antibody on its target antigen (usually, on a protein). Identification and characterization of antibody binding sites aid in the discovery and development of new therapeutics, vaccines, and diagnostics. Epitope characterization can also help elucidate the mechanism of binding for an antibody and can strengthen intellectual property (patent) protection. Experimental epitope mapping data can be incorporated into robust algorithms to facilitate in silico prediction of B-cell epitopes based on sequence and/or structural data. Epitopes are generally divided into three classes: linear, conformational and discontinuous. Linear epitopes are formed by a continuous sequence of amino acids in a protein. In conformational epitopes the binding residues are contained within certain key protein structural conformations, such as in helices, loops or beta sheets. The conformation of the epitope is important for bringing binding residues in the correct positions. Finally, discontinuous epitopes consist of parts of the antigen that are not close in the protein sequence but are brought together by three-dimensional protein folding. Discontinuous epitopes can harbour linear and conformational parts. B-cell epitope mapping studies suggest that most interactions between antigens and antibodies, particularly autoantibodies and protective antibodies (e.g., in vaccines), rely on binding to discontinuous epitopes. (en) |
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rdfs:comment | تخطيط محدد مولد الضد (بالإنجليزية: Epitop mapping) هو عملية يتم فيها تحديد مواقع الارتباط أو «التكوينات الفوقية (الإبيتوبات)» للأجسام مضادة على مولدات الضد المستهدفة والتي تكون عادة بروتينات. (ar) Die Epitopkartierung (engl. epitope mapping) beschreibt die Bestimmung von Epitopen auf Antigenen. Darunter befinden sich sowohl MHCI-gebundene Epitope, die durch den T-Zell-Rezeptor auf cytotoxischen T-Zellen erkannt werden, als auch MHCII-gebundene Epitope, die durch CD4-positive T-Zellen erkannt werden sowie lösliche oder Zellmembran-gebundene Antigene, deren Epitope durch Antikörper von B-Zellen erkannt werden. Die Epitopkartierung dient der Identifikation von immundominanten Epitopen für potentielle Impfstoffe, sowie der Entwicklung therapeutischer und diagnostischer Präparate. (de) In immunology, epitope mapping is the process of experimentally identifying the binding site, or epitope, of an antibody on its target antigen (usually, on a protein). Identification and characterization of antibody binding sites aid in the discovery and development of new therapeutics, vaccines, and diagnostics. Epitope characterization can also help elucidate the mechanism of binding for an antibody and can strengthen intellectual property (patent) protection. Experimental epitope mapping data can be incorporated into robust algorithms to facilitate in silico prediction of B-cell epitopes based on sequence and/or structural data. (en) |
rdfs:label | تخطيط محدد مولد الضد (ar) Epitopkartierung (de) Epitope mapping (en) |
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