Characterization of aid1, a Novel Gene Involved in Fusobacterium nucleatum Interspecies Interactions (original) (raw)

Abstract

The oral opportunistic pathogen Fusobacterium nucleatum is known to interact with a large number of different bacterial species residing in the oral cavity. It adheres to a variety of Gram-positive bacteria, including oral streptococci via the arginine-inhibitable adhesin RadD. In this study, we describe a novel protein encoded by the predicted open reading frame FN1253 that appears to play a role in interspecies interactions of F. nucleatum, particularly with oral streptococci and related Gram-positive species. We designated FN1253 as aid1 (Adherence Inducing Determinant 1). Expression analyses demonstrated that this gene was induced in F. nucleatum single species biofilms, while the presence of representative members of the oral microbiota known to adhere to F. nucleatum triggered its suppression. Inactivation as well as overexpression of aid1 affected the ability of F. nucleatum to coaggregate with oral streptococci and the closely related Enterococcus faecalis, but not other Gram-positive oral species tested. Furthermore, overexpression of aid1 led to a drastic change in the structure of dual species biofilms of F. nucleatum with oral streptococci. Aid1 function was abolished in the presence of arginine and found to be dependent on RadD. Interestingly, differential expression of aid1 did not affect messenger RNA and protein levels of RadD. These findings indicate that RadD-mediated adhesion to oral streptococci involves more complex cellular processes than the simple interaction of adhesins on the surface of partner strains. Aid1 could potentially play an important role in facilitating RadD-mediated interaction with oral streptococci by increasing binding specificity of F. nucleatum to other microbial species.

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Acknowledgments

The pHS58 shuttle plasmid was kindly provided by Dr. Susan Kinder Haake. This work is supported by the Whitcome Pre-doctoral Training Grant to AK and NIH/NIDCR grant DE021108 to RL.

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Authors and Affiliations

  1. UCLA Department of Microbiology, Immunology and Molecular Genetics, Los Angeles, CA, 90095, USA
    Aida Kaplan & Wenyuan Shi
  2. C3-Jian, Inc, Marina del Rey, CA, 90292, USA
    Christopher W. Kaplan & Wenyuan Shi
  3. UCLA School of Dentistry, 10833 Le Conte Avenue, CHS 33-080, Los Angeles, CA, 90095-1668, USA
    Xuesong He, Wenyuan Shi & Renate Lux
  4. UCLA David Geffen School of Medicine, Los Angeles, CA, 90095, USA
    Ian McHardy

Authors

  1. Aida Kaplan
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  2. Christopher W. Kaplan
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  3. Xuesong He
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  4. Ian McHardy
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  5. Wenyuan Shi
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  6. Renate Lux
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Corresponding author

Correspondence toRenate Lux.

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Supplemental Fig. 1

Construction of aid1 mutant strains. A. Construction of Δ_aid1_ inactivation strain. aid1 was inactivated via double homologous recombination by inserting a catP thiamphenicol/chloramphenicol resistance cassette into the aid1 gene. B. Construction of _Fn/_pEP-aid1 and _radD/pEP-aid1 overexpression strains. aid1 was overexpressed in wild type F. nucleatum ATCC 23726 and in radD strains by introducing a shuttle plasmid carrying aid1 gene with its own endogenous promoter into wild type F. nucleatum ATCC 23726 and Δ_radD strains respectively. (PPTX 52 kb)

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Kaplan, A., Kaplan, C.W., He, X. et al. Characterization of aid1, a Novel Gene Involved in Fusobacterium nucleatum Interspecies Interactions.Microb Ecol 68, 379–387 (2014). https://doi.org/10.1007/s00248-014-0400-y

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