Mahmoud Ottai - Academia.edu (original) (raw)

Papers by Mahmoud Ottai

Journal of Applied Biology & Biotechnology

World Journal of Agricultural Sciences, 2007

Australian journal of basic and applied sciences, 2011

In field trials, six flax cultivars of diverse origins were grown during three successive seasons... more In field trials, six flax cultivars of diverse origins were grown during three successive seasons with three sowing dates in each growing season. Mean squares of all traits studied across the three seasons and three sowing dates; exhibited highly significant effects for all traits recorded. The effect of sowing dates was more pronounced than seasons for all traits except No. of fruiting branches / plant, seed index as well as seed yield/ plant, straw and biological yield/ plant which due to the differences of climatic factors prevail in the three sowing dates. The first and second order interaction involving genotypes and seasons or sowing dates were significant for all traits indicating different responses of genotypes under each of variation in environmental condition. In the third order interaction significance for all studied traits were shown for the interaction between genotypes x seasons x sowing dates. Seed yielding capacity for all tested genotypes ranged from 0.53 (g) for ...

The assessment of four Nigella sativa varieties from Egypt, Iran, Turkey and Syria indicated high... more The assessment of four Nigella sativa varieties from Egypt, Iran, Turkey and Syria indicated highly significant differences in phenotypic and chemotypic characters. Seed yield was the most important character to differentiate all Nigella varieties based on high genetic advance and heritability values. In addition, the relative percentage of all detected fatty acids differed (from trace amounts to 63.691%) among varieties although the major saturated and unsaturated fatty acids were palmitic, arachnidic and linoleic acids in all varieties. The relative percentage of 8 essential and 8 nonessential amino acids different in all varieties although the Turkish variety had only 7 nonessential amino acids. Arginine and proline (ranging from 4.94 and 46.07% to 6.40 and 53.70%, respectively) were the major essential and nonessential amino acids, respectively for all varieties. SDS-PAGE electrophoresis revealed a different number of protein bands ranging from 9 bands in the Syrian variety to 1...

Five linseed (Linum usitatissimum L.) varieties, Geria, Olin, Gentiana, Midin and Deta were impor... more Five linseed (Linum usitatissimum L.) varieties, Geria, Olin, Gentiana, Midin and Deta were imported from Romania to evaluate their genetic diversity among their yield components and molecular fingerprinting RAPDPCR to insert new germplasms in a breeding program for improving the Egyptian linseed. The varietal seeds were cultivated during three successive winter seasons 2008/2009, 2009/2010 and 2010/2011 and 13 yield and yield components traits were recorded. Analysis of variance of the 1 st season cleared the significant diversity among varieties for all characters except, number of fruiting branches/plant, number of capsules/plant and seed index. In the 2 nd season, eight characters showed significant differences vs. five insignificant trait differences, while five significant trait differences and eight insignificant traits were recorded in the 3 rd season. High genetic homogeneity within the plants was attributed to Gentiana variety which presented the lowest genotypic coefficie...

Anise, Pimpinella anisum L. is an important medicinal plant in Egypt. It's essential oil has ... more Anise, Pimpinella anisum L. is an important medicinal plant in Egypt. It's essential oil has been used for the purposes of pharmaceuticals, food industry as well as its biological activity. The major aim of this study is to obtain new anise genotypes characterized with enhanced essential oil yield for utilization in a promising hybridization program in Egypt. Therefore 45 different phenotypic forms of anise plants were selected all over the cultivated area in ElMinia Governorate. The selected plants were cultivated for two successive seasons (2007/2008 and 2008/2009) and their characters were evaluated. Analysis of variance detected highly significant variation among the genotypes in all traits for both seasons. Three genotypes no. 31, 22 and 3 attracted the attention according to their owning the best mean values for all traits. Meanwhile, the coefficient of variation, broad sense heritability and genetic advance proved that essential oil yield is the most important trait for a...

Hyocyamus sp. is an important medicinal plant belonging to the Solanaceae family. It contains tro... more Hyocyamus sp. is an important medicinal plant belonging to the Solanaceae family. It contains tropane alkaloids, mainly hyoscyamine and scopolamine, which have medical usage. The primary objective of this study was to compare between proteins, isozymes as biochemical markers and RAPD-PCR molecular markers to assess the genetic diversity and relationships among five Hyoscyasmus species. SDS-PAGE for total soluble protein and PPO-isozyme analysis revealed low degree of polymorphism. In addition, some species from Hyocyamus sp. did not show any markers. A high degree of polymorphism was detected among the five Hyoscyasmus species through five random primers used for RAPD-PCR marker analysis. Fifteen monomorphic bands and 37 polymorphic distinct fragments (71.15% polymorphism) were revealed in the five Hyocyamus sp. with five primers. In addition, primer-3 and primer-5 were highly polymorphic (88.89 and 83.33% polymorphism, respectively). Moreover, primers 2, 4 and 1 were moderately pol...

Wild and inbred genotypes of Silybum marianum were assessed for five quantitative characters, sil... more Wild and inbred genotypes of Silybum marianum were assessed for five quantitative characters, silymarin production and its potent antiviral activities. Purple, white and wild varieties as well as their genotypes showed significant genetic variations in all characters except for the number of main branches/plant. Correlation coefficients showed highly significant values in all varieties while significant regression values were computed for plant height and total branches each with head flowers, in addition to seed yield with total branches in all. Six silymarin components were detected showing high variation among varieties and genotypes. Purple genotype P34 and white W9 were the best genotypes as they presented the maximum mean value for all studied characters and the highest silymarin content (30.20 and 62.85 mg/g, respectively). The seed methanolic extract of all genotypes had an inhibitory effect on Tomato mosaic virus (ToMV) infectivity. The extract reduced ToMV infection with d...

Annual Research & Review in Biology, 2017

Journal of Biological Sciences, 2017

This study was carried out during three successive seasons 2004/05, 2005/06 and 2006/07, to evalu... more This study was carried out during three successive seasons 2004/05, 2005/06 and 2006/07, to evaluate fifty F derived lines (from the F diallel cross), with three check varieties under two different irrigation regimes. 3 2 Twenty different lines were selected from the fifty F lines under each level of irrigation and evaluated under 3 the same conditions in the F and F generations. Also, the effect of irrigation regime on insect infestation of 4 5 stored grains for six F selected lines under each level was studied. F results revealed that highly significant 5 3 differences were obtained between the two irrigation regimes for all traits studied. Highly significant differences among genotypes were obtained in normal (L) and water stress conditions (L) as well as in their combined 1 2 data for all traits studied except grain yield / plant in combined data only. F results revealed that highly 5 significant differences among F selected lines were found for all traits studied at the two irr...

Journal of Microbial & Biochemical Technology, 2014

Medicinal plants are considered new resources for producing agents that could act as alternatives... more Medicinal plants are considered new resources for producing agents that could act as alternatives to antibiotics in treatment of antibiotic-resistant bacteria. The present study aimed to evaluate the efficacy of essential oil extracted from Achillea fragrantissima plant growing in Egypt for antimicrobial, antiviral activities and chemical composition analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). We also performed determination of essential oil antimicrobial activity by agar desk diffusion method and Minimal Inhibitory Concentration (MIC). Also, antiviral activity on ORF virus by pock reduction test was performed. It was reduced virus titer from 5.9 to 1.00 at 180 minutes. Detection of beta lactams resistant bacteria (Gram-positive bacteria Staphylococcus aureus, Staphylococcus epidermidis (MRSA) and Gram-negative bacteria Escherichia coli) by PCR with primers of mecA gene and Bela gene. The essential oil obtained by hydrodistillation was analyzed by GC-MS. The major components were found to be Santolina triene (1.97%), 2,5,5-trimethyl-3,6-heptadien-2-ol (8.23%) Eucalyptol 8.17, trans-2,7-Dimethyl-4,6octadien-2-ol (24.40%), 1,5-Heptadien-4-one-3,6-trimethyl (7.65%), Artemisia alcohol (3.49%), à Thujone (33.97%), Cissabinol (1.92%), Lavandulol (0.71%), 2-Octen-4-ol, 2-methyl (2.02%), 3-Cyclohexen1ol,4-methyl1 (1 methylethyl) (CAS) (2.15%), à terpineol (0.05%), Estragole (0.71%) Lavandulyl acetate (0.49%), Sabinyl acetate (2.12 %), Germacrened (0.94%). Finally, our study proved that the essential oil has bactericidal effect on some bacterial resistant antibiotic (Gram-positive bacteria Staphylococcus aureus, Staphylococcus epidermidis (MRSA) and Gram-negative bacteria Escherichia coli) as well as antiviral activity on ORF virus but it is still need further extensive studies for safety and drug interaction.

Veterinary World, 2014

Aim: Bovine mastitis is the most economically important disease affecting dairy cattle worldwide ... more Aim: Bovine mastitis is the most economically important disease affecting dairy cattle worldwide from an economic, diagnostic and public-health point of view. The present study aimed to isolate and identify of bacteria causes mastitis in dairy cows and to evaluate the antibacterial activities of some selected medicinal plants extracts comparing antibiotics used in the treatment of mastitis in Egypt. Materials and Methods: A total of 203 milk samples of dairy cows were collected during the period from February to June 2013 at different Governorates in Egypt. The use clinical inspection and California mastitis test examination were provided efficient diagnostic tool for detection of clinical, subclinical mastitis and apparently normal health cattle. The collected milk samples were cultured on Nutrient, Blood agar, Mannitol salt, Edward's and MacConkey agar plates supporting the growth of various types of bacteria for their biochemical studies and isolation. The antimicrobial activity of plants extracts (Jasonia montana and Artemisia herb alba)with different solvent (ethanol, petroleum ether, chloroform and acetone)were studied in vitro against isolated bacteria from mastitis by paper desk diffusion and minimum inhibitory concentration method (MIC). Results: The prevalence of clinical, subclinical mastitis and normal healthy animals were 34.50%, 24.7% and 40.8% respectively. The major pathogens isolated from collected milk samples were Escherichia coli (22.16%), Staphylococcus aureus (20.19%), Streptococcus spp. (13.3%), Streptococcus agalactiae (12.8%), Streptococcus dysgalactia (0.5%), Pasteurella spp. (2.45%), Klebsiella spp. (1.47%)and Pseudomonas spp. (0.45%). The highest antibacterial activity of J. montana plant extracted with acetone solvent against S. agalactiae, E. coli, S. aureus, Klebsiella spp and coagulase-negative Staphylococci with zone of inhibition values ± standard deviation (SD), ranging from 4.33±0.57 to 25.6±0.60 mm. The MIC values for the extracts ranged from 0.01 to 1.56 mg/ml. when comparing antibacterial activity of A. herb alba plant extracted with acetone solvent on the same bacteria with zone of inhibition values ± SD, ranging from 00±00 to 5.6±0.60 mm. Both extracts from J. montana and A. herb alba plant extracts with petroleum ether, methanol and chloroform solvent were less antibacterial activities than acetone solvent extract. Conclusion: The present study spot highlight on isolation and identification of mastitis pathogens that are fundamental aspects of milk quality, udder health control programs and public health and food safety issues associated with food borne pathogens. J. montana and A. herb alba plants have antibacterial effects more than antibiotics used in the treatment of mastitis. Finally, the medicinal plant extracts can be used to discover bioactive natural product in the form of antibacterial that may be serve the development of new pharmaceutical products. But still need further research necessary to identify active compounds and research to mechanism and drug interaction.

Planta Medica, 2011

Three local resources (Rajab, Haraz and Khider) of Lepidium sativum L. were used to study the gen... more Three local resources (Rajab, Haraz and Khider) of Lepidium sativum L. were used to study the genetic variation in Egypt. The study included quantitative characters, fatty acid and DNA fingerprint. Five quantitative characters were studied among three successive seasons. Separated and combined statistical analysis presented significant variation among resources and among interaction between resources and seasons in the plant characters. GLC analysis of fatty acid methyl esters were carried out for each resource. Arachidonic ...

International Journal of …, 2006

Archives Of Phytopathology And Plant Protection, 2004

ABSTRACT Three roselle, Hibiscus sabdariffa L. varieties (Sudani, Masri and White) were cultivate... more ABSTRACT Three roselle, Hibiscus sabdariffa L. varieties (Sudani, Masri and White) were cultivated at three different locations to recognize the transportation ability of roselle cultivation from the narrow old valley land to broad new land in Egypt. Qena as origin in situ old land, El-Kanater as ex situ old land and Nubaria as ex situ new land were the considered locations. Six growth quantitative characters and bolls infestation by spiny bollworm, Earias insulana were evaluated. Growth characters of roselle plants were affected significantly by either variety or location. Qena region was more suitable for roselle plant growth as judged with plant height, number of branches, number of fruits and sepals dry weight, followed by Nubaria followed by El-Kanater. Whereas, plants grown at Nubaria produced more fresh sepals weight than Qena or El-Kanater grown plants. As for Sudani, Nubaria exhibited the tallest plants, with the highest number of fruits and the heaviest fresh sepals as compared with the corresponding plants in Qena or El-Kanater. Values of broad sense heritability were highest for all characters in Qena. While the number of fruits per plant had the highest heritability in all locations. Dry sepals yield had highly significant correlation with all studied characters except percentage of water loss in Qena and Nubaria. Path coefficient analysis confirmed that fresh sepals yield had the highest direct and indirect effects on dried sepals yield. Chemical constituents responsible to sepal quality tended to produce significant variations due to the changes in varieties or locations. The highest levels of anthocyanins and sugars were achieved by Sudani variety, but the highest levels of free amino acids and total soluble solids were recorded for Masri variety. Moreover, Nubaria region was the most favourable for the accumulation of more anthocyanins in the sepals of all varieties followed by Qena. Plants grown at Qena produced sepals with the highest levels of sugars, free amino acids, organic acids and total soluble solids, followed by Nubaria followed by El-Kanater plants. Infestation with spiny bollworm Earias insulana was increased from Sudani up to Masri up to White varieties. Plants grown at Nubaria had the lowest number of attacks by bolls in all varieties, followed by those at El-Kanater followed by Qena plants. Spiny bollworm infestation was positively correlated with the number of branches and dry sepals weight, but negatively correlated with sepal moisture loss and anthocyanin contents. These findings clearly indicated that the Nubaria region was considered as a promising reclaimed area suitable for roselle cultivation, especially for Sudani, the most economic variety.

Australian Journal of Basic and Applied Sciences, 2012

Abstract: Three cultivars (Haraz, Rajab and Khider) were used to study the genetic variation of L... more Abstract: Three cultivars (Haraz, Rajab and Khider) were used to study the genetic variation of Lepidium sativum in Egypt. The study included quantitative characters for yield components, fatty acid composition and DNA fingerprint. Six quantitative characters were studied during three successive generations (parent, progeny and offspring). Separated and combined statistical analysis presented significant variation among cultivars in their characters. GC analysis of fatty methyl esters were carried out for each cultivar. ...