Bivalvia Research Papers - Academia.edu (original) (raw)

The large tropical lucinid clam Codakia orbicularis has a symbiotic relationship with intracellular, sulfide-oxidizing chemoautotrophic bacteria. The respiration strategies utilized by the symbiont were explored using integrative... more

The large tropical lucinid clam Codakia orbicularis has a symbiotic relationship with intracellular, sulfide-oxidizing chemoautotrophic bacteria. The respiration strategies utilized by the symbiont were explored using integrative techniques on mechanically purified symbionts and intact clam-symbiont associations along with habitat analysis. Previous work on a related symbiont species found in the host lucinid Lucinoma aequizonata showed that the symbionts obligately used nitrate as an electron acceptor, even under oxygenated conditions. In contrast, the symbionts of C. orbicularis use oxygen as the primary electron acceptor while evidence for nitrate respiration was lacking. Direct measurements obtained by using microelectrodes in purified symbiont suspensions showed that the symbionts consumed oxygen; this intracellular respiration was confirmed by using the redox dye CTC (5-cyano-2,3-ditolyl tetrazolium chloride). In the few intact chemosymbioses tested in previous studies, hydrog...

Previous works revealed the presence of the nPKC enzyme p105 in hemocytes of M. galloprovincialis Lmk. Specific mussel antibodies were obtained from mouse and used in confocal microscopy and Western blotting. These techniques allowed the... more

Previous works revealed the presence of the nPKC enzyme p105 in hemocytes of M. galloprovincialis Lmk. Specific mussel antibodies were obtained from mouse and used in confocal microscopy and Western blotting. These techniques allowed the observation of p105 cytosol-to-membrane translocation induced by TPA for the first time in hemocytes of molluscs. The incubation of mussel immune cells with TPA for longer than 30 min also triggered a down-regulation process. Mussel hemocytes are an excellent model to study the molecular processes of innate immunity.

Invasive species introduced via the ballast water of commercial ships cause enormous environmental and economic damage worldwide. Accurate monitoring for these often microscopic and morphologically indistinguishable species is challenging... more

Invasive species introduced via the ballast water of commercial ships cause enormous environmental and economic damage worldwide. Accurate monitoring for these often microscopic and morphologically indistinguishable species is challenging but critical for mitigating damages. We apply eDNA sampling, which involves the filtering and subsequent DNA extraction of microscopic bits of tissue suspended in water, to ballast and harbor water sampled during a commercial ship's 1400 km voyage through the North American Great Lakes. Using a lab-based gel electrophoresis assay and a rapid, field-ready light transmission spectroscopy (LTS) assay, we test for the presence of two invasive species: quagga (Dreissena bugensis) and zebra (D. polymorpha) mussels. Furthermore, we spiked a set of uninfested ballast and harbor samples with zebra mussel tissue to further test each assay's detection capabilities. In unmanipulated samples, zebra mussel was not detected, while quagga mussel was detect...

Optimization of acid digestion method for mercury determination in marine biological samples (dolphin liver, fish and mussel tissues) using a closed vessel microwave sample preparation is presented. Five digestion procedures with... more

Optimization of acid digestion method for mercury determination in marine biological samples (dolphin liver, fish and mussel tissues) using a closed vessel microwave sample preparation is presented. Five digestion procedures with different acid mixtures were investigated: the best results were obtained when the microwave-assisted digestion was based on sample dissolution with HNO3-H2SO4-K2Cr2O7 mixture. A comparison between microwave digestion and conventional reflux digestion shows there are considerable losses of mercury in the open digestion system. The microwave digestion method has been tested satisfactorily using two certified reference materials. Analytical results show a good agreement with certified values. The microwave digestion proved to be a reliable and rapid method for decomposition of biological samples in mercury determination.

Focused ultrasonic-assisted extraction (FUSE) is a new and particular technique based on the cavitation effect. In this work, the focused ultrasound assisted extraction was studied and developed for the extraction of polycyclic aromatic... more

Focused ultrasonic-assisted extraction (FUSE) is a new and particular technique based on the cavitation effect. In this work, the focused ultrasound assisted extraction was studied and developed for the extraction of polycyclic aromatic hydrocarbons from marine sediments and mussel tissues. The variables influencing the extraction (amplitude of the ultrasound pulse, the extraction time and the solvent) were studied by a full factorial design and a central composite design. As a result, flat response surfaces were obtained and the most convenient conditions were 45% of ultrasound amplitude, 120 s of extraction time and 5 mL of acetone. Both accuracy and precision of the method were evaluated by means of two certified reference materials (marine sediment and mussel tissue) and the results were also compared to those obtained by microwave assisted extraction.

Over 12 and 14 months, the sexual maturity and reproductive cycle of nailon clam Paphia textilis (Gmelin 1791) was studied respectively. P. textilis were collected off the waters of Sillon and Jilantagaan Islands, Bantayan, Cebu,... more

Over 12 and 14 months, the sexual maturity and reproductive cycle of nailon clam Paphia textilis (Gmelin 1791) was studied respectively. P. textilis were collected off the waters of Sillon and Jilantagaan Islands, Bantayan, Cebu, Philippines by hookah diving at 10 to 14 m deep. Sediments samples were collected to determine the type of substrate the nailon clam prefers. Water temperature and salinity were monitored monthly. To determine gonad development, a total of 2,501 nailon clams were examined. Morphological analysis revealed that nailon clams did not exhibit external sexual dimorphism. All samples within the size range of 40 to 76 mm were sexually mature. All stages of gonad development showed that sexually mature nailon clams can possibly spawn over a year with peaks in May, August and December. Gonad Index (GI) determines the gametogenic activities of the individual clams. High GI values ranging from 2.1 to 2.85 were obtained in all months excluding the months of June and September with GI values of 1.7 and 1.88 respectively. Condition Index (CI) determines the meat quality and the most reliable means of monitoring the changes in the reproductive activity. CI values from 5,414.44 to 7,895.11 revealed a reasonably good to very good meat quality. Poor and lean meats were observed with 3,925.17 and 4,390.55 CI values respectively. Water temperature and salinity had no significant effect on the gonad cycle. There were no wide fluctuations in water temperature and salinity between rainy and dry seasons. Paphia textilis prefers to embed in a very fine sandy substrate.

This study proposes a new method to determine the mechanical properties of human skin by the use of the indentation test [Pailler-Mattei, 2004. Caractérisation mécanique et tribologique de la peau humaine in vivo, Ph.D. Thesis, ECL-no.... more

This study proposes a new method to determine the mechanical properties of human skin by the use of the indentation test [Pailler-Mattei, 2004. Caractérisation mécanique et tribologique de la peau humaine in vivo, Ph.D. Thesis, ECL-no. 2004-31; Pailler-Mattei, Zahouani, 2004. Journal of Adhesion Science and Technology 18, 1739-1758]. The principle of the measurements consists in applying an in vivo compressive stress [Zhang et al., 1994. Proceedings of the Institution of Mechanical Engineers 208, 217-222; Bosboom et al., 2001. Journal of Biomechanics 34, 1365-1368; Oomens et al., 1984. Selected Proceedings of Meetings of European Society of Biomechanics, pp. 227-232; Oomens et al., 1987. Journal of Biomechanics 20(9), 877-885] on the skin tissue of an individual's forearm. These measurements show an increase in the normal contact force as a function of the indentation depth. The interpretation of such results usually requires a long and tedious phenomenological study. We propose a new method to determine the mechanical parameters which control the response of skin tissue. This method is threefold: experimental, numerical, and comparative. It consists combining experimental results with a numerical finite elements model in order to find out the required parameters. This process uses a scheme of extended Kalman filters (EKF) [Gu et al., 2003. Materials Science and Engineering A345, 223-233; Nakamura et al., 2000. Acta Mater 48, 4293-4306; Leustean and Rosu, 2003. Certifying Kalman filters. RIACS Technical Report 03.02, 27pp. http://gureni.cs.uiuc.edu/~grosu/download/luta + leo.pdf; Welch and Bishop, An introduction to Kalman filter, University of North Carolina at Chapel Hill, 16p. http://www.cs.unc.edu/~welch/kalman/]. The first results presented in this study correspond to a simplified numerical modeling of the global system. The skin is assumed to be a semi-infinite layer with an isotropic linear elastic mechanical behavior [Zhang et al., 1994. Proceedings of the Institution of Mechanical Engineers 208, 217-222] This analysis will be extended to more realistic models in further works.

The culmination of an eight year study, it treats all bivalve mollusks living from northern Baja California, México to northern Perú. A total of 890 species are described and illustrated with detailed color photographs and drawings. All... more

The culmination of an eight year study, it treats all bivalve mollusks living from northern Baja California, México to northern Perú. A total of 890 species are described and illustrated with detailed color photographs and drawings. All habitats in the region are included from the intertidal splash zone to the abyssal depths of the ocean basins. The book has over 6,000 complete bibliographic references to the bivalves including citations on the biology, physiology, ecology, and taxonomy of this commercially and biologically important group. Character tables and dichotomous keys assist the reader in identification. Also included in the 1400 page book is an illustrated key to the superfamiles of the region, and a complete glossary.

Creating a habitat classification and mapping system for marine and coastal ecosystems is a daunting challenge due to the complex array of habitats that shift on various spatial and temporal scales. To meet this challenge, several... more

Creating a habitat classification and mapping system for marine and coastal ecosystems is a daunting challenge due to the complex array of habitats that shift on various spatial and temporal scales. To meet this challenge, several countries have, or are developing, national classification systems and mapping protocols for marine habitats. To be effectively applied by scientists and managers it is essential that classification systems be comprehensive and incorporate pertinent physical, geological, biological, and anthropogenic habitat characteristics. Current systems tend to provide over-simplified conceptual structures that do not capture biological habitat complexity, marginalize anthropogenic features, and remain largely untested at finer scales. We propose a multi-scale hierarchical framework with a particular focus on finer scale habitat classification levels and conceptual schematics to guide habitat studies and management decisions. A case study using published data is included to compare the proposed framework with existing schemes. The example demonstrates how the proposed framework’s inclusion of user-defined variables, a combined top-down and bottom-up approach, and multi-scale hierarchical organization can facilitate examination of marine habitats and inform management decisions.

Gastropod and bivalve have a broad distribution in time and space, and mostly abundance as an individual. Generally, their habit was the sea, live along the shore or in shallow waters. The aims of this research were to examine the effect... more

Gastropod and bivalve have a broad distribution in time and space, and mostly abundance as an individual. Generally, their habit was the sea, live along the shore or in shallow waters. The aims of this research were to examine the effect of bottom substrate texture and organic material content as a detector in the presence of gastropod and bivalve in Sluke beach. Research locations were divided into five stations, every 100 meters apart along the shore. Each location was divided into 5 substations which space 100 meters each to the sea. Sampling was done by using 1 x 1 m quadrant transects, then sample was cleaned and restored for further identification. The result showed that most gastropod and bivalve found at station B2 while in stations A3, A5, C3, D3, and E2 there was no bivalve or gastropod found. The most abundant species was Nassarius siquijorensis of gastropod class as much as 62 species while the Trachycardium rugosum of bivalve class was the smallest in an amount to be found. Organic material content in Sluke Beach has a distinct range. The highest was 22.38% of high organic material in C5 while the lowest was 2.65% of low organic material in E1. The bottom substrate texture in station 1 mainly was sand, station 2 mainly silty sand. While station 3, 4, and 5 mainly were sandy silt.

We describe an early middle Miocene (late Langhian) terrestrial mollusc fauna from Nowa Wieś Królewska at Opole in Silesia (Poland). This survey is based on the rich collection stored in the Natural History Museum in Vienna and represents... more

We describe an early middle Miocene (late Langhian) terrestrial mollusc fauna from Nowa Wieś Królewska at Opole in Silesia (Poland). This survey is based on the rich collection stored in the Natural History Museum in Vienna and represents the first revision of this classical fauna. The assemblage is extraordinarily diverse and comprises 82 gastropod species and one bivalve species. About 56% of the taxa are known so far only from Nowa Wieś Królewska, emphasizing the preeminent position of this fauna within the European Neogene. The fauna reveals taxonomic affinities with early and early middle Miocene faunas. Stratigraphic overlap of several species and genera would suggest a correlation with the mammal biozone MN 5; integrating mammal data, however, indicates correlation with the biozone MN 6. This discrepancy is explained best by the lack of other adequately revised European MN 6 mollusc faunas. Consequently, the fauna from Nowa Wieś Królewska is proposed here as type of a MN 6 mollusc fauna in Central Europe. As such, this fauna represents the best-documented terrestrial mollusc fauna from the late Miocene Climate Optimum. Semi-quantitative abundance data of the terrestrial gastropods suggests the presence of ephemeral ponds and fens that formed on Cret-aceous limestones, surrounded by forested environments with leaf litter and dead wood. Occidentina gen. nov., Mennoia gen. nov., Neubertella gen. nov. and Eurocystina gen. nov. are described as new genera. Martinietta kadolskyi sp. nov., Carychium stworzewiczae sp. nov., Gastrocopta (Albinula) polonica sp. nov., Leiostyla piserai sp. nov., Vertigo antipygmaea sp. nov., Mennoia sculpturata sp. nov., Neubertella pulchra sp. nov., Aegopinella depressula sp. nov., Eurocystina nordsiecki sp. nov. and Vitrea angustaeumbilicata sp. nov. are introduced as new species. •

Treatment of the mussel Mytilus edulis with 6-hydroxydopamine or with alpha-methyl-p-tyrosine decreased dopamine and increased serotonin in the nervous system. Treatment with dopamine decreased serotonin concentrations and prevented the... more

Treatment of the mussel Mytilus edulis with 6-hydroxydopamine or with alpha-methyl-p-tyrosine decreased dopamine and increased serotonin in the nervous system. Treatment with dopamine decreased serotonin concentrations and prevented the effect of 6-hydroxydopamine. The serotonin concentration appears to be determined in part by the concentration of dopamine.

A combination of bioenergetics and biochemical biomarkers in mussels was applied to assess possible pollution impacts in a protected semi-enclosed estuary (Amvrakikos Gulf, NW Greece) that receives pesticide discharges through riverine... more

A combination of bioenergetics and biochemical biomarkers in mussels was applied to assess possible pollution impacts in a protected semi-enclosed estuary (Amvrakikos Gulf, NW Greece) that receives pesticide discharges through riverine transport. Scope for growth, a physiological condition index representing the energy budget of the organism, was applied to detect general stress effects on the health status of mussels. The low energy budgets of mussels revealed stress conditions and provided early warning signals of possible consequences at higher levels of biological organization. Biochemical markers of exposure confirmed a risk of pesticide contamination. Decreased acetylcholinesterase activities indicated exposure to organophosphate and carbamate pesticides. Responses of the antioxidant enzyme glutathione peroxidase suggested the presence of contaminants capable of reactive oxygen species production that could be related to organochlorine pesticide contamination in the area. On the other hand, metallothionein levels implied low metal contamination.