Plant tissue Culture Techniques Research Papers (original) (raw)

Top Papers
Most Cited Papers
Most Downloaded Papers
Newest Papers
People
- by
- •
- Embryology, Morphology, Morphogenesis, Growth
- by Sheela Chandra
- •
- Engineering, Technology, Biological Sciences, Tissue culture
- by Igor Besson
- •
- Anthropology, Plant tissue Culture Techniques

The production of erythroidines and other alkaloids was studied in cotyledons, callus and cell suspension cultures of Erythrina americana Miller. The cell suspension cultures, grown in Murashige & Skoog medium with naphthaleneacetic acid (3 mg l(-1)) and kinetin (2 mg l(-1)), produced 89 and 17 microg alpha- and beta-erythroidines respectively per g dry wt.

- by Ana Ramos-Valdivia
- •
- Engineering, Technology, Biotechnology, Plant Tissue Culture
- by Dan Longo
- •
- Cancer, Humans, Natural Killer cells, Plant tissue Culture Techniques
- by Guillaume Mabilleau
- •
- Biomaterials, Hydroxyapatite, Scanning Electron Microscopy, Transmission Electron Microscopy
- by Hemmo Drexhage and +3
- •
- Humans, Tissue culture, Female, Plant tissue Culture Techniques
- by Eike Martin
- •
- Microcirculation, Brain, Animals, Resuscitation
- by ence darmo
- •
- Production, Regeneration, Plant Biology, Culture
- by H. Shiozaki
- •
- Survival Analysis, Probability, Humans, Female
- by N. Szentandrássy and +4
- •
- Skeletal muscle biology, Kinetics, Patch-clamp and imaging techniques, Antioxidants
- by Nosisa Dube
- •
- Gene transfer techniques, Plant tissue Culture Techniques, Somatic Embryogenesis, Germination
- by Simon Brookes
- •
- Gastroenterology, Motor neuron, Immunohistochemistry, Small Mammals
- by John Elsworth
- •
- Psychology, Dopamine, Cercopithecus aethiops, Caudate Nucleus
- by elsa zotta
- •
- Water, Gene expression, Pregnancy, Humans
- by Man Singh
- •
- Testosterone, Female, Animals, Plant tissue Culture Techniques
- by John Mayer
- •
- Tissue Engineering, Sheep, Animals, Plant tissue Culture Techniques
- by Suraiya Rasheed
- •
- Electron Microscopy, Cancer, RNA viruses, Cell line
- by Salvatore De Rosa
- •
- Engineering, Technology, Biotechnology, Porifera
- by A. Kirakosyan
- •
- Phytochemistry, Medicinal Plants, Biological Sciences, Plant tissue Culture Techniques
- by antony jeevarajan
- •
- Bioengineering, Biotechnology, Multidisciplinary, Cell Culture
- by Andrei Erofeev
- •
- Wound Healing, Face, Solid state lasers, Humans
- by Megan Cavet
- •
- Safety, Contact Lenses, Cell line, Humans
- by Victor Govardovskii
- •
- Kinetics, Vision, Animals, Plant tissue Culture Techniques
- by Yves Ndong
- •
- Regeneration, Plant Biology, Potato, High Frequency
- by Luis Solchaga
- •
- Biomedical Engineering, Tissue Engineering, Osseointegration, Cell Adhesion
- by Zhang Yan
- •
- British, Drug interactions, Humans, Plant tissue Culture Techniques
- by Tiina M Nieminen
- •
- Analytical Chemistry, Graphite, Calibration, Plant tissue Culture Techniques
- by Neal Pellis and +1
- •
- Tissue Engineering, Multidisciplinary, Cellular mechanotransduction, Humans
- by Olav Vintermyr
- •
- Dentistry, Immunohistochemistry, Morphogenesis, Cell Differentiation
- by LokaBharathi Ponnapakkam
- •
- Fungi, Biodiversity, Porifera, Humans
- by Ismael Silva
- •
- Immunohistochemistry, Humans, Female, Plant tissue Culture Techniques

• The aim of this study was to establish the histological effects of exposure to microcystin-LR (MC-LR), a cyanotoxin, on axenic Phragmites australis plantlets.• Plantlets were regenerated from embryogenic reed calli by tissue culture methods.• Microcystin-LR inhibited the growth and development of embryogenic calli and the growth of reed plantlets. The 50% plantlet growth inhibitory concentration value (IC50) of MC-LR was 12 µg ml−1 (12.07 µm) on mineral medium and 36 µg ml−1 (36.22 µm) on Murashige-Skoog medium. In the case of roots, the IC50 value was 4.1 µg ml−1 (4.12 µm) on both media. Microcystin-LR induced aerenchyma obturation, altered lignification of cell walls in the axial organs, root necrosis and the capture of lateral or adventitious roots in the tissues of axial organs of reed plantlets. Cyanotoxin induced the premature development of lateral roots, root coalescence and early aerenchyma formation.• Our data suggest that microcystin-LR, a cyanotoxin, induced developmental and histological alterations leading to growth inhibition of reed, and the induced harms have an impact on understanding reed decay in eutrophic fresh waters.The aim of this study was to establish the histological effects of exposure to microcystin-LR (MC-LR), a cyanotoxin, on axenic Phragmites australis plantlets.Plantlets were regenerated from embryogenic reed calli by tissue culture methods.Microcystin-LR inhibited the growth and development of embryogenic calli and the growth of reed plantlets. The 50% plantlet growth inhibitory concentration value (IC50) of MC-LR was 12 µg ml−1 (12.07 µm) on mineral medium and 36 µg ml−1 (36.22 µm) on Murashige-Skoog medium. In the case of roots, the IC50 value was 4.1 µg ml−1 (4.12 µm) on both media. Microcystin-LR induced aerenchyma obturation, altered lignification of cell walls in the axial organs, root necrosis and the capture of lateral or adventitious roots in the tissues of axial organs of reed plantlets. Cyanotoxin induced the premature development of lateral roots, root coalescence and early aerenchyma formation.Our data suggest that microcystin-LR, a cyanotoxin, induced developmental and histological alterations leading to growth inhibition of reed, and the induced harms have an impact on understanding reed decay in eutrophic fresh waters.

- by Csaba Máthé
- •
- Regeneration, Eutrophication, Biological Sciences, Plant tissue Culture Techniques
- by David Sandall
- •
- Science, Multidisciplinary, Cell line, Biomedical Research
- by Benoit Roussel
- •
- Aging, Glutamate, Brain, Endothelial Cells
- by Hemant lata
- •
- Regeneration, Complementary and Alternative Medicine, Plant Biology, High Frequency

Background and objectives: Developing scaffolds is important for tissue engineering and repairing damaged tissues. The present study aimed to investigate effects of pre-incubation of an electrospun silk fibroin scaffold in complete and serum-free media on proliferation and survival of cells seeded on the scaffold. Methods: After removing sericin from the silk cocoon and preparing the fibroin solution (3% w/v), the electrospun silk fibroin scaffold was fabricated and its morphology was evaluated by scanning electron microscopy. The scaffolds were pre-incubated in complete and serum-free Dulbecco's Modified Eagle media for one hour (short-term) and 10 days (long-term), and the hydrophilicity of scaffolds was evaluated by measuring the water contact angle. Rat bone marrow mesenchymal stem cells were seeded onto the scaffolds, and cell survival and genomic DNA concentration were evaluated after 21 days. Results: The short-time pre-incubation of electrospun silk fibroin scaffolds in the complete medium increased the proliferation of seeded cells because of serum protein adsorption. In addition, long-term pre-incubation of the scaffolds in the complete and serumfree media increased cell proliferation due to the increased hydrophilicity of the scaffold (p<0.05). However, only long-term pre-incubation of the scaffolds in the complete medium had a significant effect on cell survival. Conclusion: The results demonstrated that long-term pre-incubation of the scaffolds in the complete medium have more profound positive effects on cell survival and proliferation compared to short-term pre-incubation.

- by Medical Laboratory Journal MLJ and +1
- •
- Plant tissue Culture Techniques, Blood Proteins
- by Silvia Alves
- •
- Pharmacology, Biochemistry, Bioinformatics, Endocrinology

Chemical studies on Hamelia patens (Rubiaceae) micropropagated plantlets allowed production of a new monoterpenoid oxindole alkaloid, named (–)-hameline (7), together with eight known alkaloids, tetrahydroalstonine (1), aricine (2), pteropodine (3), isopteropodine (4), uncarine F (5), speciophylline (6), palmirine (8), and rumberine (9). The structure of the new alkaloid was assigned on the basis of 1D and 2D NMR spectroscopy, mass spectrometry, and molecular modeling.

- by Ana Ramos-Valdivia
- •
- Chemistry, Organic Chemistry, Plant Biology, Medicine
- by Pascale Gaudet
- •
- Pharmacology, Biochemistry, Bioinformatics, Endocrinology

SISN=12905 Call Number : SB320.3.G8. Author : Gopalakrishnan, TR; Title : Cassava (Manihot esculenta Crantz). Publisher : Kerala: Kerala Agricultural University,. Source : Three decades of vegetable research in Kerala Agricultural... more

- by Vijay Koya
- •
- Pharmacology, Biochemistry, Bioinformatics, Endocrinology
- by Hina Fazal
- •
- Technology, Kinetics, Biomass, Biological Sciences

Lactate dehydrogenase (LDH) isozyme 1 (B4), 5 (A4), and “X” (Testis or Sperm type) have been partially purified from mouse tissues.The following studies were carried out on the three isozymes: Km and optimum substrate concentration for pyruvate, α-oxo-butyrate, α-oxo-valerate, lactate, α-OH-butyrate, and α-OH-valerate, inhibition by substrate and product, effect of malate, N-(4-carboxy-2-hydroxyphenyl) maleimide, some citric acid cycle metabolites, urea, trypsin and pH.The mouse testicular LDH isozyme clearly differs from the common lactate dehydrogenases and from the tesficular isozymes from other species. It shows distinct sensitivity to inhibition by substrate or product whether the direct (pyruvate lactate) or reverse reactions are studied. There is no effect of increasing concentrations of pyruvate or lactate on the direct reaction, while a clear inhibition by lactate or pyruvate is demonstrated on the reverse reaction. Citric acid cycle metabolites, specially malate and succinate, inhibit the direct reaction catalyzed by the “X” isozyme.These peculiar characteristics suggest a high degree of specialization for the principal testicular isozyme of lactate dehydrogenase.

- by Antonio Blanco
- •
- Evolutionary Biology, Genetics, Zoology, Catalysis
- by glyn stacey
- •
- Quality Control, Cell Culture, Biological Sciences, Tissue culture
- by Javier Marcos
- •
- Molecular Biology, Fungi, Humans, Animals
- by Rodrigo Hasbun
- •
- Epigenomics, Plant tissue Culture Techniques, DNA methylation, Chromatin

We have come a long way since the days, early in the century, when Dr. EV McCollum, to the amazement and con-sternation of his fellow Kansan farmers, raided corncribs for mice and rats to use for nutrition experiments. McCollum believed... more

- by Myra Sklarew
- •
- Mathematics, Toxicology, Environmental Sciences, Humans