COMPARITIVE EVALUATION OF EFFICACY OF CALCIUM HYDROXIDE, TRIPLE ANTIBIOTIC PASTE AND PROPOLIS AS INTRACANAL MEDICAMENT AGAINST ENTEROCOCCUS FAECALIS (original) (raw)
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International Journal of Advanced Research (IJAR), 2019
The study aimed to evaluate the potential effect of three intracanal medicaments namely propolis, Calcium hydroxide and Chlorhexidine gel on the root canal disinfection. Materials and method: Sixty-Four samples were chosen for this study. They were divided into four groups, group I received no medication, group II received propolis, group III received Calcium Hydroxide and group IV received Chlorhexidine. The four groups were subdivided into two subgroups A and B, with two-time intervals 1 and 2 weeks. For bacterial evaluation the samples were inoculated with Enterococcus Faecalis. Paper points were placed inside infected root canals and placed in a test tube for CFU count. Results: Results showed that propolis had an intermediate effect on Enterococcus faecalis due to the presence of phenolic compounds in propolis which can eliminated bacteria inside root canal. Calcium hydroxide showed an intermediate effect against bacteria due to its high alkalinity that also can eliminate bacteria. Chlorhexidine showed the highest effect against bacteria due to its ability to stay active inside canal for a long time (substantivity). These results were similar for the 2 time periods. Conclusion: Both Propolis and Ca(OH)2 showed moderate action on intracanal microbes over the two time periods and CHX can be regarded as the best intracanal medicaments regarding its action on intracanal microbes.
Journal of Endodontics, 2011
The aim of this study was to evaluate and compare the antimicrobial activity of calcium hydroxide, triantibiotic mixture (TAM), and an ethanol extract of propolis as intracanal medicaments on Enterococcus faecalis–infected root canals.One hundred twenty extracted intact human permanent incisors were decoronated, and chemomechanical preparation of the root canal was performed. After sterilization of the samples, they were inoculated with pure culture of E. faecalis and incubated. After incubation, colony-forming units were recorded before medication. Then, samples were divided randomly into five groups (n = 24). Each group was then exposed to various intracanal medicaments, namely calcium hydroxide (group 1), TAM (group 2), propolis (group 3), ethanol (group 4), and saline as the control group (group V). The antibacterial effectiveness of the different intracanal medicament was recorded by determining the percentage reduction in colony counts (%RCC) at the end of days 1, 2, and 7. The data were statistically analyzed using one-way analysis of variance and Tukey Honestly Significant Difference (HSD) post hoc test.The %RCC was highest for propolis showing 100% reduction on day 2 followed by TAM showing 82.5%, 92.2%, and 98.4% of reduction on days 1, 2, and 3, respectively. Calcium hydroxide showed a gradual increase in antibacterial activity with a maximum of 59.4% on day 7.Propolis was more effective than TAM against E. faecalis at a 2-day time period, and both were equally effective at 7 days.
2017
Introduction: Debridement of root canal using appropriately safe and effective irrigants is the key factor for long-term success. Purpose of this study was to compare the antibacterial effect of propolis with 5.25% sodium hypochlorite, and 2% chlorhexidine against enterococcus faecalis. Materials &Methods: In this study, 36 single-canal roots were used. The crown was removed and instrumentation was prepared by step-back technique, then teeth were sterilized and contaminated with E. Faecalis, and divided into four groups with 9 cases: group1: Propolis, group2: 5.25% sodium hypochlorite, group3: 2% chlorhexidine and group4: controls. Irrigants were injected by a 27-gauge syringe and roots were incubated in 37°C for one week. Sampling was done and inoculated to tryptone soy broth media, after 24 hours the turbidity was measured. Samples were also cultured on agar plates, and colony-forming units were counted as CFU/ml. Data were analysed using the Mann-Whitney test. Results: The differ...
Background and Aim: Propolis, a natural product of the honeybee, is currently used as an anti-inflammatory and antimicrobial agent. Using different antibacterial agents is an important step to reduce the number of microorganisms within the root canal and improve the endodontic treatment prognosis. The present in vitro study investigated the antibacterial efficacy of Propolis against Enterococcus faecalis compared to calcium hydroxide. Materials & Methods: In this experimental study, 42 single-rooted human teeth were selected and their smear layer was completely removed after access cavity and canal preparation. After infecting the prepared canals with Enterococcus faecalis species, negative control group was used during sterilization period and in two groups of 18 each, canals were filled with 30%propolis extract and calcium hydroxide, respectively. No material was added to the positive control group. The specimens were stored in CO2 incubator for 72 hours, 1 week and 1 month and af...
Introduction: Enterococcus faecalis (E. faecalis) is a microorganism commonly detected in persistent endodontic infections. Eradication of this organism from an infected root canal remains a significant clinical challenge for modern endodontic treatment. So, this in vitro study was conducted to compare antimicrobial effect of some root canal medicaments on E. faecalis. Materials/Methods: A total of 50 E. faecalis strains (49 strains were selected randomly from positive cultures together with a standard E. faecalis strain ATCC 29212) were used in the study. Inoculum for each bacterial strain was prepared in a turbidity of 0.5 on McFarland scale. The prepared discs of the three intracanal medicaments; chlorhexidine, diapex and diclofenac; together with readymade discs of amoxicillin and doxycycline were distributed on blood agar and Mueller-Hinton agar plates inoculated with an E. faecalis suspension previously prepared. Antimicrobial activity was assessed by measuring the diameters of the zones of growth inhibition surround the discs after incubation at 37°C for 24 hours and then after 48 hours. Results: All strains (100%) were sensitive to chlorhexidine even though strains that showed resistance to one or both of antibiotic used (amoxicillin, doxycycline). On the other hand, all strains (100%) were resistant to diapex and diclofenac even standard strain. Sixty eight percent of the tested strains were sensitive to doxycyline and 44% of them were sensitive to amoxicillin. Conclusion: Under the conditions of this study, chlorhexidine is very effective against E. faecalis and can be considered as the most powerful root canal medicament compared to other intracanal medicaments tested. Controversially, no zones of inhibition were detected around diapex and diclofenac. Keywords: Enterococcus Faecalis; Root Canal Medicaments; Antibacterial Effect; Chlorhexidine; Diapex; Diclofenac; Amoxicillin; Doxycycline.
2021
AIM To evaluate and compare the antimicrobial efficacy of Ca(OH)2, 25% propolis, and 25% Glycyrrhiza glabra as intracanal medicaments in root canal treatment. MATERIALS AND METHODS Total 60 freshly extracted permanent incisors were decoronated and chemomechanical preparation of root canal was performed. Samples were inoculated with a pure culture of Enterococcus faecalis and incubated for 21 days. Colony-forming units (CFUs) were recorded before medication. Incubated samples were randomly categorized into three groups, namely, Ca(OH)2, propolis, and G. glabra, with 20 samples in each group. Antibacterial activity was assessed by evaluating the variance in the CFUs on Day 7. Paired "t" test and Post-hoc Tukey's test were applied to analyze the data. RESULTS Reduction of CFUs was noticed in all the groups (p <0.001), however the reduction was more predominant in the propolis group. CONCLUSION Propolis is more effective against E. faecalis, when compared to G. glabra a...