Scanning Transmission Electron Microscopy Research Papers (original) (raw)

The effect of 5-aminoimidazole-4-carboxamide-ribonucleoside (AICAR) activation of the AMP-activated protein kinase (AMPK) on the transport of the model radiolabeled dipeptide [3H]-D-Phe-L-Gln was investigated in the human epithelial colon cancer cell line Caco-2. Uptake and transepithelial fluxes of [3H]-D-Phe-L-Gln were carried out in differentiated Caco-2 cell monolayers, and hPepT1 and glucose transporter 2 (GLUT2) protein levels were quantified by immunogold electron microscopy. AICAR treatment of Caco-2 cells significantly inhibited apical [3H]-D-Phe-L-Gln uptake, matched by a decrease in brush-border membrane hPepT1 protein but with a concomitant increase in the facilitated glucose transporter GLUT2. A restructuring of the apical brush-border membrane was seen by electron microscopy. The hPepT1-mediated transepithelial (A-to-B) peptide flux across the Caco-2 monolayers showed no significant alteration in AICAR-treated cells. The electrical resistance in the AICAR-treated monol...

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- Physiology, Kinetics, Scanning Transmission Electron Microscopy, Humans

Biological and technical failures of implants have already been reported. Mechanical factors are certainly of importance in implant failures, even if their exact nature has not yet been established. The abutment screw fracture or loosening represents a rare, but quite unpleasant failure. The aim of the present research is an analysis and structural examination of screw thread or abutment loosening compared with screw threads or abutment without loosening. The loosening of screw threads was compared to screw thread without loosening of three different implant systems; Branemark (Nobel Biocare, Gothenburg, Sweden), T.B.R. implant systems (Benax, Ancona, Italy) and Restore (Lifecore Biomedical, Chaska, Minnesota, USA). In this study broken screws were excluded. A total of 16 screw thread loosenings were observed (Group I) (4 Branemark, 4 T.B.R and 5 Restore), 10 screw threads without loosening were removed (Group II), and 6 screw threads as received by the manufacturer (unused) (Group ...

In the treatment of photoaged skin, glycolic acid works by removing superficial portions of the epidermis and stimulating dermis regeneration. Vitamins A, C, and E should stimulate collagen production and antioxidants should prevent free radical damage and skin aging. However, the effectiveness of different therapies has often relied on subjective methods of assessment. Histologic analysis has seldom been used because of the drawback of permanent scarring. In the literature, the use of a quantitative method for the assessment of facial rejuvenation has been described: the silicone replica technique. The authors' aim was to promote and recommend the use of this technique and, in particular, to test the effect of glycolic acid and multivitamin- and antioxidant-based products on skin texture. The authors performed a prospective, randomized, double-blind, controlled study on 30 women treated topically in the outer canthal region (crow's-feet area). Patients were divided into thr...

Acid mine drainage (AMD) – characterized by high acidity and elevated sulfate and metal concentrations – represents a big environmental concern. Biological sulfate reduction has become an alternative to the classical physicochemical methods. In this study, domestic wastewater (DW) was tested as a cost-effective carbon-source for the remediation of AMD. Sediments from Tinto River, an extreme acidic environment with an elevated concentration of metals, were used as inoculum. Three anaerobic bioreactors with different microbial supports were fed with a 1:10 (v:v) mixture of synthetic AMD:DW. Around 50% of the organic matter present in the DW co-precipitated with the metals from the AMD previous to feeding the reactor. Therefore, the reactors had to be supplemented with an extra carbon-source (acetate) to achieve higher S elimination. Elevated removal efficiencies of chemical oxygen demand (COD) (>88%), sulfate (>75%), Fe (>85%) and other dissolved metals (>99% except for Mn...

Physical activity modulates inflammation and immune response in both normal and pathologic conditions. We investigated whether regular and moderate exercise before the induction of experimental sepsis reduces the risk of lung and distal organ injury and survival. One hundred twenty-four BALB/c mice were randomly assigned to two groups: sedentary (S) and trained (T). Animals in T group ran on a motorized treadmill, at moderate intensity, 5% grade, 30 min/day, 3 times a week for 8 wk. Cardiac adaptation to exercise was evaluated using echocardiography. Systolic volume and left ventricular mass were increased in T compared with S group. Both T and S groups were further randomized either to sepsis induced by cecal ligation and puncture surgery (CLP) or sham operation (control). After 24 h, lung mechanics and histology, the degree of cell apoptosis in lung, heart, kidney, liver, and small intestine villi, and interleukin (IL)-6, KC (IL-8 murine functional homolog), IL-1β, IL-10, and numb...

- by Andrea Toledo
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- Zoology, Biology, Scanning Transmission Electron Microscopy, Medicine
- by Sigmund Andersen
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- Engineering, Electron Microscopy, Epitaxial Growth, Applied Physics
- by Tsung-Sheng Kang
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- Engineering, III-V Semiconductors, Applied Physics, Scanning Electron Microscopy

Doxorubicin (DOX)-loaded water-in-oil-in-water (W/O/W) microemulsions were produced using a shirasu-porous-glass (SPG) membrane emulsification technique. Soybean oil was used as the oil phase; polyglycerol polyricinoleate (PGPR) or tetraglycerol polyricinoleate (TGPR) was used as the surfactant to stabilize the feed W/O emulsions, while Tween 20 was used in the external water phase to stabilize oil droplets containing water droplets. Increasing the feed pressure from 50 to 90 kPa increased the particle size of W/O/W emulsions, whereas it was decreased by increasing the agitator speed. The smallest particle sizes of multiple emulsions were obtained at the feed pressure of 50 kPa and agitator speed of 350 rpm. Under this set of conditions, the increase in the concentration of PGPR or TGPR showed a decrease in the particle size of DOX-loaded W/O/W emulsions. The optimized formulation comprising of 5% w/v PGPR and 3% w/v Tween 20 in the oil phase and external water phase, respectively, ...

- by Roshan Pradhan
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- Water, Pharmacokinetics, Scanning Transmission Electron Microscopy, Oils

The importance of breakfast cereal flakes (BCF) in Western diets deserves an understanding of changes in their mechanical properties and microstructure that occur during soaking in a liquid (that is, milk or water) prior to consumption. The maximum rupture force (RF) of 2 types of breakfast flaked products (BFP)--corn flakes (CF) and quinoa flakes (QF)--were measured directly while immersed in milk with 2% of fat content (milk 2%) or distilled water for different periods of time between 5 and 300 s. Under similar soaking conditions, QF presented higher RF values than CF. Soaked flakes were freeze-dried and their cross section and surface examined by scanning electron microscopy. Three consecutive periods (fast, gradual, and slow reduction of RF) were associated with changes in the microstructure of flakes. These changes were more pronounced in distilled water than in milk 2%, probably because the fat and other solids in milk become deposited on the flakes' surface hindering liqu...

- by Juan Carlos braulio Manabí condori
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- Chemical Engineering, Food Science, Microstructure, Water

We present a novel approach for analysis of low-conductivity and insulating materials with conventional pulsed-voltage atom probe tomography (APT), by incorporating an ultrathin metallic coating on focused ion beam prepared needle-shaped specimens. Finite element electrostatic simulations of coated atom probe specimens were performed, which suggest remarkable improvement in uniform voltage distribution and subsequent field evaporation of the insulated samples with a metallic coating of approximately 10nm thickness. Using design of experiment technique, an experimental investigation was performed to study physical vapor deposition coating of needle specimens with end tip radii less than 100nm. The final geometries of the coated APT specimens were characterized with high-resolution scanning electron microscopy and transmission electron microscopy, and an empirical model was proposed to determine the optimal coating thickness for a given specimen size. The optimal coating strategy was ...

In the development of new antibacterial therapeutic approaches to fight multidrug-resistant bacteria, antimicrobial photodynamic therapy (aPDT) represents a well-known alternative to treat local infections caused by different microorganisms. Here we present a polypropylene (PP) fabric finished with citrate-hydroxypropyl-βCD polymer (PP-CD) entrapping the tetra-anionic 5,10,15,20-tetrakis(4-sulfonatophenyl)-21H,23H-porphine (TPPS) as photosensitizer eluting scaffold (PP-CD/TPPS) for aPDT. The concept is based on host-guest complexation of porphyrin in the cavities of CDs immobilized on the PP fibers, followed by its sustained and controlled delivery in release medium and simultaneous photo-inactivation of microorganisms. Morphology of fabric was characterized by optical (OM) and scanning electron microscopies (SEM). Optical properties were investigated by UV-vis absorption, steady- and time-resolved fluorescence emission spectroscopy. X-ray Photoelectron Spectroscopy (XPS) and FT-IR ...

- by Mariachiara Trapani
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- Engineering, Chemistry, Textiles, Scanning Electron Microscopy

The size of gold nanoparticles (AuNPs) can influence various aspects of their cellular uptake. Light microscopy is not capable of resolving most AuNPs, while electron microscopy (EM) is not practically capable of acquiring the necessary statistical data from many cells and the results may suffer from various artifacts. Here, we demonstrate the use of a fast EM method for obtaining high-resolution data from a much larger population of cells than is usually feasible with conventional EM. A549 (human lung carcinoma) cells were subjected to uptake protocols with 10, 15, or 30 nm diameter AuNPs with adsorbed serum proteins. After 20 min, 24 h, or 45 h, the cells were fixed and imaged in whole in a thin layer of liquid water with environmental scanning electron microscopy equipped with a scanning transmission electron microscopy detector. The fast preparation and imaging of 145 whole cells in liquid allowed collection of nanoscale data within an exceptionally small amount of time of ~80 h...

We have investigated the functional role of the non-helical end domains of vimentin on its assembly properties using truncated Xenopus and human recombinant proteins. Removal of the amino-terminal “head” domain yielded a molecule that did not assemble into 10 nm filaments but remained in a soluble oligomeric particle form with a sedimentation coefficient considerably smaller than that of wild-type vimentin

- by Kenneth Goldie
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- Chemistry, Molecular Biology, Calcium, Scanning Transmission Electron Microscopy
- by Joerg Mayer
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- Engineering, Electrochemistry, Carbon, Titanium
- by Alberto Villa and +1
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- Engineering, Nanoparticle, Scanning Transmission Electron Microscopy, Activated Carbon
- by kanokporn Pangsomboon
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- Zoology, Dentistry, Biomedical Engineering, Scanning Electron Microscopy
- by Juan Carlos Condori
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- Chemical Engineering, Food Science, Microstructure, Water
- by Achim Schmalenberger and +1
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- Geology, Geochemistry, Kinetics, Scanning Transmission Electron Microscopy
- by Sandwip Dey
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- Engineering, Materials Science, Applied Physics, Scanning Electron Microscopy

Carbon Nanotubes (CNTs) and Metal Organic Frameworks (MOFs) represent two of the most promising classes of emerging materials in engineering today. Together, they exhibit many useful properties such as high conductivity, catalytic reactivity , porosity , and strength. Methods in integrating these materials towards electromagnetic and chemical applications is of strategic importance for defense. Innovative materials have often been designed by combining low and high dimension constituents, through compositing, as a means to improving the functionality. In this work, we were able to combine these nanomaterials by synthesizing a covalently bonded MOF-CNT nanostructured composites, using zirconium containing MOF (MOF-808).

- by jeevan reddy
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- Engineering, Materials Science, Technology, Materials

The outer surface of the facet lenses in the compound eyes of moths consists of an array of excessive cuticular protuberances, termed corneal nipples. We have investigated the moth-eye corneal nipple array of the facet lenses of 19 diurnal butterfly species by scanning electron microscopy, transmission electron microscopy and atomic force microscope, as well as by optical modelling. The nipples appeared to be arranged in domains with almost crystalline, hexagonal packing. The nipple distances were found to vary only slightly, ranging from about 180 to 240 nm, but the nipple heights varied between 0 (papilionids) and 230 nm (a nymphalid), in good agreement with previous work. The nipples create an interface with a gradient refractive index between that of air and the facet lens material, because their distance is distinctly smaller than the wavelength of light. The gradient in the refractive index was deduced from effective medium theory. By dividing the height of the nipple layer in...

Olympus BX53 Digital Microscope

ÖZET Bu araştırma atom altı parçacıkların görüntülenmesinde kullanılan teknolojilerden olan AFM(Atomik force microscope) ve SEM(Scanning electron microscope) incelenmiştir. Bu inceleme mikroskopların nasıl çalıştığı, çalışma sırasında kullanılan materyaller ve çalışma sonunda ne gibi bilgiler verdikleri yönündedir. Her bir mikroskobun kendine göre avantaj ve dezavantajları irdelenip çalışma içerisinde paylaşılmıştır. Mikroskopların kullanım alanları ve hangi araştırmalarda tercih edildikleri bu çalışmada yer almaktadır. Mikroskopların çalışma aksamları ve özelliklerin incelenmiştir. Aynı zamanda mikroskoplar kendi aralarında karşılaştırılmıştır. Mikroskoplar hakkındaki teorik bilgiyi giriş bölümünde, uygulama alanları ve karşılaştırmaları ise AFM&SEM ve SONUÇ bölümünde bulabilirsiniz.

Immunohistochemical Staining Methods Education Guide - Sixth Edition

Special Stains and H&E Education Guide

Immunohistochemical Staining Method

Grain boundaries are intrinsic and omnipresent microstructural imperfections in polycrystalline and nanocrystalline materials. They are short-circuit diffusion paths and preferential locations for alloying elements, dopants, and impurities segregation. They also facilitate heterogeneous nucleation and the growth of secondary phases. Therefore, grain boundaries strongly influence many materials' properties and their stabilities during application. Here, we propose an approach to measure diffusion, segregation, and segregation-induced precipitation at grain boundaries at a sub-nanometer scale by combining atom probe tomography and scanning transmission electron microscopy. Nanocrystalline multilayer thin films with columnar grain structure were used as a model system as they offer a large area of random highangle grain boundaries and inherent short diffusion distance. Our results show that the fast diffusion flux proceeds primarily through the core region of the grain boundary, which is around 1 nm. While the spatial range that the segregated solute atoms occupied is larger: below the saturation level, it is 1,2 nm; as the segregation saturates, it is 2-3.4 nm in most grain boundary areas. Above 3.4 nm, secondary phase nuclei seem to form. The observed distributions of the solutes at the matrix grain boundaries evidence that even at a single grain boundary, different regions accommodate different amounts of solute atoms and promote secondary phase nuclei with different compositions, which is caused by its complex threedimensional topology.

FLEX Ready to Use Atlas of Stains

- by Radost Pascova
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- Materials Engineering, Fracture, Glass, Modeling and Simulation

Histostaining Atlas of Special Stains

IHC World Histology Services Price List

- by Stefania Albonetti and +1
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- Materials Engineering, Kinetics, Nanoparticle, Scanning Transmission Electron Microscopy

Next-generation high-performance structural materials are required for lightweight design strategies and advanced energy applications. Maraging steels, combining a martensite matrix with nanoprecipitates, are a class of high-strength materials with the potential for matching these demands. Their outstanding strength originates from semi-coherent precipitates, which unavoidably exhibit a heterogeneous distribution that creates large coherency strains, which in turn may promote crack initiation under load. Here we report a counterintuitive strategy for the design of ultrastrong steel alloys by high-density nanoprecipitation with minimal lattice misfit. We found that these highly dispersed, fully coherent precipitates (that is, the crystal lattice of the precipitates is almost the same as that of the surrounding matrix), showing very low lattice misfit with the matrix and high anti-phase boundary energy, strengthen alloys without sacrificing ductility. Such low lattice misfit (0.03 ± 0...

- by Dierk Raabe
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- Materials Science, Titanium, Nanoparticles, Scanning Transmission Electron Microscopy

The thermal degradation of some sulfonic cationites namely Amberlite IR-120, Indion-223 and Indion-225 was investigated using instrumental techniques like thermal analysis (TG) and Scanning Electron Micros-copy (SEM). Fourier Transform Infrared Spectroscopy (FTIR) was used to characterize the resins degradation steps. The sulfonic cationites undergo degradation through dehydration, followed by decomposition of sulfonic acid functional groups liberating SO 2. The thermogravimetric analysis of above cationites at higher temperature up to 520˚C, show mass loss of 61.61% and 25.43% respectively for Indion-223 and Indion-225, while Amberlite IR-120 cationite get burned off completely.

- by Norzita Ngadi
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- Engineering, Materials Science, Technology, Materials

An upper temperature limit of 450 degrees C has been established for growth of heteroepitaxial Si1-x-yGexCy solid solutions with substitutional C on Si(100) by combined ion and molecular beam deposition (CIMD). At 450 degrees C infrared absorption spectroscopy shows that C is on substitutional sites and no SiC precipitates are detected, whereas at 560 degrees C the substitutional C signal is much smaller but SiC precipitates are still not detected. High resolution transmission electron microscopy shows that Si1-x-yGexCy films deposited at 560 degrees C exhibit Ge deficient, coherent, secondary phase clusters in the cubic diamond matrix, which are not seen in films deposited at 450 degrees C. These observations suggest that the clusters are C-rich, Ge-deficient precursors to SiC, with a lattice which is distorted but free of extended defects. Ion channeling results indicate that the Si1-x-yGexCy films might have a distribution of different bond lengths. (C) 1996

- by Harald Jacobsson and +1
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- Electrical Engineering, Electronic Engineering, Microelectronics And Semiconductor Engineering, Engineering Physics

We investigated the effectiveness of SiN and SiON barrier layer in controlling the interfacial reaction between Atomic Layer Deposited (ALD) HfO2 film and the Si substrate. The HfO2 film was found to form silicate and silicide at the interface with Si after 5 min post deposition annealing in Ar at 800 and 1000 °C as observed by scanning transmission electron microscopy/electron energy

- by Jozef Janovec and +1
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- Materials Engineering, Mechanical Engineering, Electron Diffraction, Scanning Transmission Electron Microscopy