Thrombolytic Activity Research Papers - Academia.edu (original) (raw)

The present study was designed to investigate the Phytochemical, cytotoxic and thrombolytic activity of methanolic extract of leaves of Dysophylla auricularia. Methanolic extract of Dysophylla auricularia was used to evaluate various phytochemical methods, cytotoxicity in Brine shrimp lethality bioassay where vincristine sulphate was used as standard drug. Thrombolytic effect of the fraction was investigated in clot lysis experiment. In Brine shrimp lethality bioassay, LC50 value of the extract was 4.18537μg/ml and vincristine sulphate served as the positive control showed LC50 value 10.51μg/ml. So, compared to vincristine sulphate, it is evident that the methanolic extract of leaves of Dysophylla auricularia was cytotoxic. The extract exerted 42.1208% lysis of the blood clot in thrombolytic activity test while 75.18% and 15.82% lysis were obtained for positive control (streptokinase) and negative control respectively. So, the extract possessed considerable thrombolytic activity. The phytochemicals evaluation indicates the presence of chemical constituents including Alkaloids, Glycosides, Tannins and Flavonoids. Our present investigations indicate that methanolic extract of leaves of Dysophylla auricularia exhibited considerable cytotoxic and thrombolytic activity. There may be many pharmacologically active constituents in the fraction. So, extensive pharmacological and phytochemical experiments are essential.

Objective: To investigate the Effects of Antidiarrheal and Thrombolytic Activities of methanol extract of Cinnamomum cecidodaphne Meissn. Barks. Castor oil induced diarrhoea test were used to examine the in-vivo antidiarrheal activity in Swiss albino mice. Results: In vitro clot lysis model was undertaken to investigate the thrombolytic action of the extract. Data were analysed by using statistical software statistical package for social science (SPSS, version 19.0). In this study of antidiarrhel activity Loperamide was used as a standard. For thrombolytic test Streptokinase used as positive control. In case of antidiarrheal study value was found 76.36% 400mg/kg where standard was found 83.33% and the extract shown 35.08% of thrombolysis effect where streptokinase was found 77.06% of clot lysis effect. Conclusion: The extract showed significant activity as compare to standard drugs. Methanol extract of C. cecidodaphne Meissn barks might be triggering the premonition of novel drug discovery in future due to its antidiarrheal effect in animal model.

- by Naymul Karim
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- Antidiarrhoeal activity, Thrombolytic Activity
- by RINTI BARUA
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- Pharmacology, Hemostasis and Thrombosis, Medicinal Plants, Cytotoxicity

Ficus racemosa is a traditional medicinal plant found in Southeast Asia, India, Australia and. It is commonly known as 'Dumur.' Owing to the presence of β-sitosterol it decreases the blood glucose concentration. Many active constituents isolated from different parts of this plant exhibit useful pharmacological activity. The objective of this dissertation is to identify the biological activity of the roots of an indigenous medicinal plant, viz., Ficus racemosa (Family: Moraceae) and to evaluate the possible phytochemical and pharmacological profiles of the crude extracts. Some chemical and biological researches on this plant have been conducted out so far, focusing mainly on the plant's bark and root. That's why the objective of this framework is to assess the different possibilities of developing new therapeutic targets from this plant's fruit that could be crucial for the treatment of many diseases.

Myocardial or cerebral infractions are the serious consequences in Atherothrombotic diseases leading to death and the side effects produced by consecutive use of thrombolytic agent like t-PA, Urokinase and streptokinase to treat these diseases has become a global concern. Bacopa monnieri has been used for centuries in Ayurveda system of medicine as a memory vitalizer and as a liver and heart tonic. The present study was carried out to evaluate the thrombolytic activity of ethanolic, methanolic, acetone and aqueous extract of different parts (root, stem and leaf) of B.monnieri by in vitro method. Extraction was carried out using Soxhlet apparatus. The leaf ethanolic extract showed highest thrombolysis followed by aqueous, methanol and acetone extract. Concentration of phytochemicals and incubation time were directly proportional to the clot lysis. Application of the present study may be accessible for greater section of the society for treatment of cardiovascular diseases.

- by JPR Solutions
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- Pharmacology, Pharmacy, Herbals, Pharmacognosy

Objective: The present study was designed to investigate the thrombolytic activity of methanolic extract of leaves of Mesua ferrea. Method: Methanolic extract of Mesua ferrea.Was used to evaluate various phytochemical methods. Thrombolytic effect of the fraction was investigated in clot lysis experiment. Result: The extract exerted 36.32% lysis of the blood clot in thrombolytic activity test while 85.45% and 7.5% lysis were obtained for positive control (streptokinase) and negative control respectively. So, the extract possessed considerable thrombolytic activity. Conclusion: Our present investigations indicate that methanolic extract of leaves of Mesua ferrea exhibited considerable thrombolytic activity. There may be many pharmacologically active constituents in the fraction. So, extensive pharmacological and phytochemical experiments are essential.

- by Naymul Karim
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- Thrombolytic Activity

To analyze in vivo neuro-pharmacological effects of Alpinia nigra as anxiety is a particular form of behavioral inhibition that occurs in response to novel environmental events. In present study, the extract of Alpinia nigra was evaluated for its central nervous system depressant effect using mice behavioral models, such as hole cross, open field and thiopental sodium induced sleeping time tests for its sedative properties and an elevated plus-maze test for its anxiolytic potential, respectively. In anxiolytic study, the extract displayed increased percentage of entry into open arm at the dose of 400 and 200 mg/kg. The extract produced a significant (P<0.01) increase in sleeping duration and reduction of onset of sleep compared to sodium thiopental at both doses (200 and 400 mg/kg). The extract (200 and 400 mg/kg) also showed a dose-dependent suppression of motor activity and exploratory activity of the mice in both open field and hole cross test. This study demonstrates that the...

- by Dr. A. M. Abu Ahmed
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- Pharmacology, Biochemistry, Chemistry, Pharmacy

Atherothrombotic diseases or coronary artery thrombosis are common disorders which are treated by streptokinase (SK), urokinase (UK) or tissue plasminogen activators (t-PA). Because of the high risk of bleeding, severe anaphylactic shock, intracranial hemorrhage and lacks of specificity of these drugs, they are restricted to those patients whohave undergone surgery or those with a history of gastrointestinal bleeding or hypertension. Therefore, plant based drugs are used because they are cheap, safe, low side effects and effective against many diseases. The study was carried out to check the clot lysis effect and cytotoxic effects of Mentha arvensis L., Mentha spicata L. and Mentha viridis L. using streptokinase as a positive control and water as a negative control. In this experiment, the M. arvensis, M. spicata and M. viridis showed 32.56%, 30.89%, 30.29% clot lysis activity in case of methanol extract, 32.04%, 30.37%, 30.02% clot lysis activity in case of ethanol extract, 31.87%,...

- by Noman Patwary
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- Pharmacology, Hemostasis and Thrombosis, Medicinal Plants, Cytotoxicity

The study was aimed to investigate the brine shrimp lethality bioassay and thrombolytic activity of the methanolic extract of Macaranga denticulata leaves. An in vitro thrombolytic model was used to evaluate the clot lysis effect of different extracts of M. denticulata along with Streptokinase as a positive control and distilled water as a negative control. The cytotoxic activity of methanolic extracts of M. denticulata leaves was evaluated by Brine shrimp lethality bioassay. The brine shrimp lethality bioassay result was (LC50=82.74 µg/ml) compared with standard vincristine sulphate (LC50=0.839 µg/ml). It has significant thrombolytic activity (34.77%) compared to standard streptokinase (70%). The results of this study confirmed that this plant candidate for future research of anticancer and thrombolytic drugs.

- by MD.IBRAHIM TAREK
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- Thrombolytic Activity

Objectives: Investigation with the crude ethanolic extract of Argyria captiformis leaves was carried out to evaluate its possible thrombolysis and to analyze in –vivo neuro-pharmacological effects as anxiety is a particular form of behavioral inhibition that occurs in response to novel environment events and also phytochemical screening of plant extract. Method: Ethanolic extract of Argyria captiformis Leaves was assessed for sedative and anxiolytic activity on Swiss albino mice and Thrombolytic activity was assessed with human blood and also phytochemical screening test was done by various chemical reagents. Sedative activity was evaluated by using hole cross, open field, thiopental sodium-induced sleeping time and anxiolytic property was evaluated by elevated-plus maze(EPM) tests at 400mg/kg while the peripheral and thrombolytic activity determined by percentage of clot lysis. Result: In anxiolytic study, the extract displayed increased percentage of entry into open arm at the dose of 400mg/kg. The extract produced a significant (P<0.01) increase in sleeping duration and reduction of onset of sleep compared to sodium thiopental at doses (400 mg/kg) .The extract (400 mg/kg) also showed suppression of motor activity and exploratory activity of the mice in both open field and hole cross test. Argyria captiformis alone & Argyria captiformis in combination with Streptokinase demonstrated 38.19±4.76% & 77.45±2.97% clot lysis effect respectively & revealed significant with comparison to both the control agent. The presence of tannins, glycosides, saponins, flavonoids, cardiac glycosides, and phytosterols was determined. Conclusion: The pharmacological profiles of the present investigation of the ethanol extract of A. captiformis indicate that the extract possess good CNS depressant and exhibited considerable thrombolytic as it significantly reduced locomotion, onset of sleep, increased duration of sleep and also presence of, glycosides, cardiac glycosides, saponins, flavonoids ,tannins and phytosterols.

- by Naymul Karim
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- Pharmacology, Biochemistry, Pharmacy, Toxicology

Atherothrombotic diseases or coronary artery thrombosis are common disorders which are treated by streptokinase (SK), urokinase (UK) or tissue plasminogen activators (t-PA). Because of the high risk of bleeding, severe anaphylactic shock, intracranial hemorrhage and lacks of specificity of these drugs, they are restricted to those patients who have undergone surgery or those with a history of gastrointestinal bleeding or hypertension. Therefore, plant based drugs are used because they are cheap, safe, low side effects and effective against many diseases.
The aim of the study was to investigate herbal preparation (aqueous extract) for their clot lysis property (thrombolytic activity) and cytotoxic properties by using In vitro models.

- by Shah Md. Shahik and +2
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- Medicinal Plants, Cytotoxicity, Mentha Spicata, Pharmacology and toxicology