Thrombolytic Activity Research Papers - Academia.edu (original) (raw)

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- Biochemistry, Organic Chemistry, Phytochemistry, Traditional Medicine
- by Daniel Simon
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- Biochemistry, Biotechnology, Nanoparticles, Medicine
- by Prof. Nurul Absar
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- Pharmacology, Chemistry, Traditional Medicine, Multidisciplinary

Background: The purpose of the investigation was to ascertain whether the leaf extracts of Anogeissus acuminata holds any significant medicinal properties. Methods: Leaves of A. acuminata was extracted with pure ethanol (EEAA), then methanol extract fractioned with n-hexane (NHFEEAA), chloroform (CHFEEAA), ethyl acetate (EAFEEAA) and methanol (MFEEAA). Cytotoxic assay was done by using brine shrimp lethality bioassay for measuring LC50. Results: Ethanol extract and its different fractions of leaves of A. acuminata showed moderate to well cytotoxic effect. In brine shrimp lethality bioassay, MFEEAA and CHFEEAA revealed the highest cytotoxic activity having LC50 values 215.32 ± 3.02 g/ml and 234.56 ± 2.01 g/ml, respectively. Conclusions: The overall results of the study indicated significant cytotoxic activity of ethanol extract and its different fractions of leaves of A. acuminata. Furthermore, this plant deserves further investigation for other paramount pharmacological activities...

- by Jahid Hassan
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- Chemistry, Pharmacy, Traditional Medicine, Ethanol

- by arafat uddin
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- Chemistry, Pharmacy, Traditional Medicine, Ethanol

The cactus (Opuntia ficus-indica (L.) Mill.) belongs to the Cactaceae family and it’s used in traditional folk medicine in treating a number of diseases and conditions. Due to the remarkable biological activity and to the bioactive (phytochemicals) compounds of O. ficus-indica, it becomes the aim of many research studies. The current study aimed to evaluate the analgesic activity of various solvent fractions (aqueous and ethanol) prepared from the O. ficus-indica flowers. The centrally analgesic potential was evaluated using tail flick latency in tail immersion and hot plate methods in mice. Morphine was used as a positive control at a dose of 3 mg kg-1, s.c.. Intra-peritoneal administration of the aqueous extract of O. ficus-indica flowers at the highest dose did not produce any toxicity symptoms, thus the median lethal dose (LD50) was estimated to be greater than 2,500 mg kg-1. The results of the pain behavior evaluation according to the gender approach of mice showed that the pai...

- by Souad Benaich
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- Traditional Medicine, Ficus, Analgesic

Costus speciosus is a flowering plant of spiral ginger family. The present study was carried out to evaluate the anxiolytic activity of Costus speciosus flower. The anxiolytic activity was studied using two methods (Elevated plus maze and Hole board test). The methanolic extracts were administered to swiss albino mice orally at doses of 200 and 400 mg/kg. The extract at dose of 400 mg/kg increased the time spent in the open arm of Elevated plus maze and the number of head dipping in hole board was increased at doses of 200 and 400 mg/kg. The results reveal the presence of anxiolytic activity of Costus speciosus flower.

- by Bibi Humayra Khanam
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- Chemistry, Cytotoxicity, Plant sciences, Dichloromethane
- by giash uddin
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- Chemistry, Cytotoxicity, Plant sciences, Dichloromethane
- by Muhammad Asad Uz Zaman
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- Chemistry, Biology, Protease, Enzyme

In recent years, plants derived phytochemicals attracting interest of researchers due to the antimicrobial/antibacterial resistance of the antibiotic drugs against several diseases; as medications from medicinal plants are safe, effective, easily available and infrequently accompanied. This review, highlighted significance of the secondary metabolites and their mode of action. Drug discovery using natural products is a challenging task for designing new leads. It describes the bioactive compounds derived from natural resources, its phytochemical analysis, characterization and pharmacological investigation. It focuses on the success of these resources in the process of finding and discovering new and effective drug compounds that can be useful for controlling infectious diseases. From many years, natural products have been acting as a source of therapeutic agents and have shown beneficial uses. The phytochemical screening of several plants using advanced analytical techniques showed ...

- by Dr. Rafia Saeed Chemistry
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- Biology, Drug, Food Technology and Nutrition Science

- by Jawaid Akram Bukhari
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- Biology, Drug, Food Technology and Nutrition Science
- by Jawaid Akram Bukhari
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- Biology, Drug, Food Technology and Nutrition Science

Objective: Terminalia chebula fruits are used as traditional herbal medicine from the ancient era but still now, the extract has not revealed any research data on CNS depression activity as well as its lyophilized herbal formulation. The present study was designed to evaluate CNS depression activity and develop lyophilized oral cake of Terminalia chebula fruit extract. Methods: CNS depression potential of Terminalia chebula fruit was examined using the hole board, hole cross, and thiopental sodium induced sleeping time test. The human equivalent dose was calculated based on US FDA guidelines of dose conversion between animals and humans. A novel lyophilized herbal oral cake of aqueous extract of Terminalia chebula fruits with additives was then formulated. The formulated cake was subjected to characterize its physicochemical properties such as appearance, residual humidity, drug content, dissolution, and drug release profile, extract-polymer compatibility by Fourier-transform infrar...

- by muhammad masum billah
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- Chemistry, Traditional Medicine, Medicine, Combretaceae

Atherothrombosis is the main cause of cardiovascular diseases. Cardiovascular diseases (CVDs) take the lives of 17.7 million people every year, 31% of all global deaths due to the Heart attacks and strokes caused by a vessel blockage by thrombus. Thrombolytic drugs are available in market, however, they cause some side effects and expensive. Recently, Meliponiculture in India has become popular because the honey has a unique taste, a mix of sweet, sour and bitter which is highly valued. Therefore, present research work carried out to investigate the phytochemical compositions of stingless bee honey and to evaluate In vitro thrombolytic activity of honey of stingless honeybee (Tetragonula iridipennis) using plasma clot method. Clot lysis is check at two concentrations of honey against the artificially prepared plasma clot. Streptokinase used as standard and sterilized distilled water used as control. The percentage of clot lysis was analysed by computing the difference between weights of clot. The test honey at concentration 1ml/10ml and 1ml/5ml showed 9.958 ± 0.860% and 4.397 ± 0.452% clot lysis respectively. Our findings showed that stingless bee honey contains varied phytochemicals-tannins, alkaloids, flavonoids, terpenoids, carotenoids, coumarins, saponins. The stingless bee honey has the potential to dissolve clot, therefore, it is urgent need to isolate the active molecule which is therapeutically beneficial in future use.

- by Hemant B Zope and +1
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- In Vitro, Honey, Honeybees, Phytochemical

not availableBangladesh Pharmaceutical Journal 19(2): 233-236, 2016

The study is regarded for standardization of aerial parts of the plant Phyllanthus maderaspatensis L., phytochemical investigations, isolation of flavonoids and evaluation for thrombolytic activity of alcoholic extract and butanolic fraction of alcoholic extract. Standardization study includes macroscopy, microscopy and determination of proximate values and phytochemical investigations include extraction of coarsely powdered herb with exhaustive alcoholic and aqueous extractions and successive extraction with petroleum ether, chloroform and alcohol. The extracts were subjected to qualitative chemical analysis, chromatographic studies [Thin Layer Chromatography (TLC) and High Performance Thin Layer Chromatography (HPTLC)] were performed for flavonoids detection in alcoholic extract. Isolated two flavonoids from butanolic fraction of alcoholic extract by column chromatography and preparative TLC, their partial characterization was done with Proton Nuclear Magnetic Resonance (PNMR) and Fourrier Transform Infrared (FT-IR) spectral data. Thrombolytic activity of alcoholic extract and butanolic fraction of alcoholic extract (20 and 10 mg/100 µl) were evaluated in incubated blood. An attempt made to standardize aerial parts of P. maderaspatensis L., was successful. Two flavonoids were isolated; the alcoholic extract showed significant thrombolytic effect in comparison with thrombosis control in comparison with standard thrombolytic agent streptokinase (30000 unit/100 µl).

- by Sajan Maharjan
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- Pharmacognosy and Phytochemistry, Thrombolytic Activity

Objective: The present investigation for the first time evaluated the in vitro fibrinolytic and thrombolytic activities of crude extracts from Eutyphoeus gammiei, native, large size earthworm of Tripura, Northeast, India. The present study was designed to evaluate the therapeutic use of the organism E. gammiei as a source of fibrinolytic and thrombolytic agent(s).Methods: The fibrinolytic activity was studied using by fibrin plate and zymography assays. Thrombolytic assay was carried out according to Prasad et al. (2006) using whole blood.Results: The results obtained clearly indicated E. gammiei as a potential source of fibrinolytic and thrombolytic agents. Both in fibrin plate assay and thrombolytic assay with whole blood, E. gammiei crude homogenate showed similar and close results in respect to that of streptokinase. Fibrin zymography also showed antifibrinolytic activity with producing clear bands. Dose and time dependency also is evident from the results.Conclusion: The result...

- by Prof. Samir Kumar Sil
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- Pharmacology, Biology, Fibrin, North East India

Tinospora cordifolia Miers. is used in the Ayurvedic system of medicine for the treatment of jaundice, diabetes, and rheumatoid arthritis, and is also used as memory enhancer. But still there is no report of analgesic and neuropharmacological activities of the plant. For this reason, the present study was designed to evaluate analgesic and neuropharmacological activities of methanol extract of the aerial parts of Tinospora cordifolia. Analgesic activity of the crude extract at the dose of 200 and 400 mg/kg b.w. was evaluated for its central and peripheral pharmacological actions using hotplate and tail flick tests and acetic acid-induced writhing test respectively in mice. The extract produced a significant (p < 0.05-0.001) increase in pain threshold in hotplate and tail flick tests in a dose dependent manner. In acetic acid-induced writhing test the extract at both doses produced significant (p < 0.001) inhibition of writhing reaction but maximum inhibition (65.01%) of writhi...

- by Aminul Haque
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- Pharmacology, Chemistry, Traditional Medicine, Medicine
- by Vasudeo Kulkarni
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- Chemistry, Multidisciplinary, Current Science, Copper

BackgroundHuman involvement in the production and processing of medicinal plants and herbal distillates caused a potential risk of microbial contamination, particularly with Staphylococcus aureus. The present research was performed to assess the prevalence and phenotypic and genotypic properties of antibiotic resistance of S. aureus bacteria isolated from diverse kinds of powdered packaged medicinal plant and bottle herbal distillate samples. MethodsThree-hundred different powdered packaged medicinal plant and bottle herbal distillate samples produced in traditional conditions were collected and examined by the culture method. Phenotypic and genotypic patterns of antibiotic resistance of S. aureus isolates were examined using disk diffusion and PCR techniques. ResultsThirty out of three-hundred (10%) powdered packaged medicinal plant and bottle herbal distillate samples were contaminated with S. aureus. The prevalence of S. aureus amongst the powdered packaged medicinal plant and bo...

- by Zohreh Mashak
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- Antibiotic Resistance, Staphylococcus aureus, Antimicrobial

This study aimed to evaluate the in vitro thrombolytic potential of Mimosa pudica (Makahiya) using the crude extract of the plant. This study utilized randomized controlled trial to determine the thrombolytic potential of Mimosa pudica (Makahiya) plant extract on the Citrated Platelet Poor Plasma. Makahiya plant was subjected to the phytochemical screening tests to determine the presence of glycosides. The study utilized twenty-one (21) healthy human subjects with normal Prothrombin time results to ensure that no recent or history of oral or intravenous anticoagulation therapy was taken. The volunteers’ blood sample were collected and subjected to centrifugation to yield citrated platelet-poor plasma. This study utilized the Euglobulin Clot Lysis Time to determine the thrombolytic potential of Mimosa pudica (Makahiya). 0.5 mL of citrated platelet poor plasma was placed into 3 test tubes labeled as positive control, experimental control, and negative control. The plasma was diluted and acidified to precipitate the euglobulin fraction. The tubes were added with 25 μL of thrombin to induce clot formation and the stopwatch was started immediately. Once clot was formed, test tube 1 was added with the 25 μL of Makahiya plant extract, and served as the experimental control. Test tube 2 was added with 25 μL of Streptokinase which served as the positive control. Test tube 3 only contained the citrated platelet poor plasma and served as the negative control.
The mean percent euglobulin clot lysis time in minutes of blood samples treated with Makahiya plant extract, Streptokinase, and untreated blood samples are 3.27 + .15, 2.33 + 3.7, and 360 respectively. Wilcoxon signed ranks test revealed that there was a significant difference between the median euglobulin clot lysis time in minutes of the blood samples treated with Makahiya extract and Streptokinase at .05 level of significance, z = -2.371 and p < .05. On the average, blood samples treated with makahiya extract (Median = 3.18) had longer euglobulin clot lysis time compared to the blood samples treated with streptokinase (Median = 2.54).
Based on the findings, Mimosa pudica (Makahiya) plant crude extract had an in vitro thrombolytic potential on citrated platelet poor plasma.

- by marshy belle
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- In Vitro, Mimosa Pudica, Thrombolytic Activity, IN-VITRO THROMBOLYTIC ACTIVITY

Atherothrombotic, which is an atherosclerotic inflammation with thrombus, is characterized by damage to blood vessels that causes myocardial infarction and isemic stroke with thrombolytic treatment. The side effects of thrombolytic treatment are the cause of the importance of alternative medicine from natural ingredients. The ethyl acetate fraction in Phyllanthus niruri L. is suspected to have thrombolytic activity due to the content of coumarin and flavonoid bioactive compounds based on research that has been done. The purpose of this study was to determine the thrombolytic activity of the ethyl acetate fraction of Phyllanthus niruri L. Thrombolytic activity test is done by measuring the percentage of clot lysis. This study used a true experimental method with a Post Test Only Control Group Design model in 6 treatments, positive control, negative control (PBS and 0.5% DMSO) and four samples for both tests, namely 250 g/mL, 500 g/mL, 1000 g/mL, 2000 g/mL. The results of statistical ...

- by Aina Azkia
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- by Aina Azkia
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- by Shivakumar S Godipurge
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- Technology, Traditional Medicine, Biology, Medicinal Plants
- by Minhajul Islam
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- Chemistry, Traditional Medicine, Biology, In Vitro
- by Md Minhajul Islam
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- Chemistry, Traditional Medicine, Biology, In Vitro

Background Campsis radicans L. is a flowering plant in Bangladesh, traditionally used for the treatment of several human diseases. In this study, in vitro antioxidant, thrombolytic, membrane stabilizing and in vivo analgesic, hypoglycemic, anti-diarrheal and CNS antidepressant activities of organic soluble fractions of crude methanol extract of C. radicans leaf were investigated using appropriate experimental models. Methods The leaves of C. radicans were collected, authenticated, dried and extracted with methanol at room temperature for 30 days. The concentrated methanol extract was partitioned to petroleum-ether (PESF), dichloromethane (DMSF) and ethyl acetate (EASF) soluble fractions. The antioxidant activity of these fractions was determined by DPPH free radical scavenging method. Total phenolic content was determined by the Folin-Ciocalteau’s spectrophotometric method. The thrombolytic activity was assessed by measuring clot lysis ability whereas the membrane stabilizing activi...

- by Md. Mirazul Islam Islam
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- Chemistry, In Vitro, Antioxidant, In Vivo
- by Minhajul Islam
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- Chemistry, Traditional Medicine, Biology, In Vitro

Aims: The present study aimed to investigate antidiarrheal and thrombolytic effect of ethanolic extract of leaves of A. dealbatum in mice. Study design: Antidiarrheal effect was evaluated by castor oil-induced diarrhea method at two different concentrations in mice and in vitro thrombolytic activity was analyzed with clot lysis assay of human blood. Place and duration of study: Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh, between December 2018 and February 2019. Methodology: The male Swiss mice’s were divided into four groups (n = 5). First group was orally treated with 1% Tween-80 (10 ml/kg) and second group was orally treated with loperamide (5 mg/kg). Third and fourth group were orally treated with ethanolic extract of leaves of A. dealbatum at 200 and 400 mg/kg accordingly. Human RBCs were collected for conducting thrombolytic assay. During this study, 1.5 ml of venous blood was drawn from healthy volunteers (n = 10) a...

- by Md.Abdul Barek
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- Traditional Medicine, Biology, In Vitro, In Vivo

In the present study the methanolic extract of Borassus flabellifer L. (MBF) was evaluated for the safety along with neuropharmacological activity by using OECD guidelines, y-maze, elevated plus-maze and open field test respectively. Mortality, sign of any toxicity or behavioral changes were not noticed up to the dose as high as 4000mg/kg. Moreover, in y-maze test, MBF 200 mg/kg and MBF 400 mg/kg exhibited anti-depressive and depressive activities. Again, every extract including both lower and higher doses elicited depressive, antidepressive and anxiolytic activities in elevated plus maze test. Later, in open field test, significant (p<0.05, vs. control) CNS depressive activities were found by both MBF 200 mg/kg and MBF 400 mg/kg. The results obtained in the present study demonstrated that MBF can be the possible sources of CNS depressant, antidepressant and anxiolytic agents. But further investigation is needed for the identification of the active compounds as well as confirmati...

- by Irin Akter
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- Traditional Medicine, Biology, Pharmacognosy and Phytochemistry, Open Field

Objective: The current study is an attempt to screen for the in vitro clot lysis and proteolytic activity of aqueous extract of Leucas aspera leaves. Methods: Thrombolytic activity and protease activity of the crude enzyme obtained by ammonium sulfate precipitation and dialysis were assayed using blood clot and casein as substrates respectively. Native PAGE and gel documentation studies were performed to calculate the molecular weight of the enzyme. Results: In the study, 40% salt fractioned crude enzyme sample exhibited significant thrombolytic and caseinolytic activity. Further dosedependent increased activity was observed with the maximum lytic activity of 52.11±1.04 % at 1 mg/ml of the sample when compared to the reference drug streptokinase (71.39±0.32%). Also, 68.72±0.62 U/hr of caseinolytic activity was observed for 1 mg/ml of the sample fraction. Conclusion: The study highlights and validates the efficacy of Leucas aspera leaves extract for thrombolytic and proteolytic actions. Enzyme with an approximate molecular weight, 19.89 KDa could be responsible for the significant lytic activity.

Background The aim of this experiment was to evaluate the cytotoxic, thrombolytic, analgesic, sedative-hypnotic and anxiolytic activities of the methanolic extract of Ficus cunia leaves. Methods Primary phytochemical screening was accomplished by using established methods. Cytotoxicity was studied by brine shrimp lethality test, and the thrombolytic assay was conducted through clot lysis method with human blood. The in vivo action was done using mice of both sexes. The analgesic activity was evaluated by acetic acid-induced writhing test and formalin-induced paw licking test. Open field, hole cross and thiopental Na-induced sleeping time test were used to examine the sedative-hypnotic activity, and elevated plus maze (EPM) and hole board test were used to identify the anxiolytic activity. Results The results elicited that the extract contained several phytochemicals such as alkaloid, flavonoid, and tannin. The extract was found to have a median lethal concentration (LC50) value of 5...

- by Alam Ansari
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- Pharmacology, Medicine, Brine shrimp, Anxiolytic

The Water-Soluble Extract (WSE) is a crude bioactive phytoconstituent of Nigella sativa (L.) seeds discovered recently. The current findings report about the thrombolytic and cytotoxic effects of WSE using human blood clot lysis and brine shrimp lethality (BSL) bioassay. The thrombolytic effect of WSE (1,666.67 µg/mL) was determined via the clot and lysate weight measurements compared to streptokinase (STK) of 30,000 IU/mL and normal saline (NS) while the cytotoxicity of WSE (44.14-2,000 µg/mL) against vincristine sulfate (VCS;3.125-100 µg/mL). WSE has shown extremely statistically significant (p<0.0001) clot lysis (90.00%) compared to NS (3.76%) whilst it was also significantly different (p<0.0063) to STK (72.41%) exhibiting LC50 of 1,795.90 µg/mL vs. VCS (39.25 µg/mL) in a dose-dependent manner. The current results suggested WSE has a potent thrombolytic effect with mild dose-dependent cytotoxicity towards brine shrimp nauplii (Artemia salina). It also suggested WSE might ha...

- by Md. Mohotasin Hossain
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- Pharmacology, Chemistry, Cytotoxicity, Fibrin
- by Md. Mohotasin Hossain
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- Botany, Chemistry, Traditional Medicine, Approximate Reasoning
- by Ý Trần
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- Traditional Medicine, In Vitro, Phytochemical, Methanol

The existing study was made to investigate the thrombolytic exercise and cytotoxic potential with the methanol extract of Syzygium operculatum leaves. The cytotoxicity had been assessed while using brine shrimp lethality bioassay and also thrombolytic impact with individual blood. The brine shrimp lethality bioassay was employed to evaluate cytotoxicity (LC50=272.82μg/ml) compared to vincristine associated with sulphate (LC50=0.512μg/ml). It had been also assessed as thrombolytic agent compared to streptokinase. It's got Significant thrombolytic exercise (36. 28%) compared to standard streptokinase (75.09%).

- by Tanvir Chowdhury
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- Pharmacology, Chemistry, Cytotoxicity, Medicine

The study was aimed to investigate the brine shrimp lethality bioassay and thrombolytic activity of the methanolic extract of Macaranga denticulata leaves. An in vitro thrombolytic model was used to evaluate the clot lysis effect of different extracts of M. denticulata along with Streptokinase as a positive control and distilled water as a negative control. The cytotoxic activity of methanolic extracts of M. denticulata leaves was evaluated by Brine shrimp lethality bioassay. The brine shrimp lethality bioassay result was (LC50=82.74 µg/ml) compared with standard vincristine sulphate (LC50=0.839 µg/ml). It has significant thrombolytic activity (34.77%) compared to standard streptokinase (70%). The results of this study confirmed that this plant candidate for future research of anticancer and thrombolytic drugs.

- by Tanvir Chowdhury
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- Biology, Pharmacognosy and Phytochemistry, Bioassay, Brine shrimp

not availableBangladesh Pharmaceutical Journal 19(2): 233-236, 2016

- by aslam hossain
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- Biology, future of pharmaceutical industry in Bangladesh

The Ardisia colorata is one of the rare hill-tract plants in Bangladesh. The experimental research was intended to evaluate the phytochemical and antimicrobial activity of 70% ethanolic extract of leaves and bark of Ardisia colorata. Preliminary phytochemical screening of both the leaf and bark extract revealed the presence of various classes of compounds such as saponins, reducing sugars, tannins, and terpenoids with minor presence of alkaloid and flavonoid. When antimicrobial study was carried out against 10 different strains of microorganisms by detecting the zone of inhibition with disc diffusion technique, the two extracts showed very strong effect especially against Vibrio parahemolyticus and Bacillus subtilis with trace activity against Salmonella typhi. When further quantitative estimation was carried out using Minimum Inhibitory Concentration (MIC) with alternate methodology involving incorporation of phenol red indicator, their corresponding MIC numerical values were deduc...

- by Reham Mostafa
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- Traditional Medicine, Biology, Phytochemical, Antimicrobial

Aims: The present study was performed to evaluate the phytochemical screening of compounds, thrombolytic activity, analgesic activity and antidiarrhoeal activity of the methanolic extract of of Cucumis sativus Linn. (Cucurbitaceae) leaves. Background: Cucumis sativus Linn. is generally disseminated all through the world especially in Asia, Africa and South America. Locally the plant is utilized for basic role of cerebral pain, the seed is utilized for diuretic and cooling purposes. The leaf sap is emetic, it is utilized for treating dyspepsia in children. Methods: The thrombolytic activity was evaluated by using clot lysis method. Analgesic activity was investigated using writhing method in mice and anti-diarrhoeal activity test was performed castor oil-induced diarrhoea in mice as well. Result: Significant thrombolytic activity was found for Cucumis sativus Linn 100mg plant leaves extract (29.33%) clot lysis in respect of positive control streptokinase (30000 IU) was shown 65.26% c...

- by Fahadul Islam
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The methanolic extract of the leaves of Smilax lanceifolia Roxb. (Family-Smilacaceae) was subjected to pharmacological investigation to ascertain analgesic and antibacterial activity.. The phytochemical screening demonstrated the presence of different types compound like anthraquinone glycoside, alkaloids, terpenoids, tannins, carbohydrate,flavone aglycone, saponin, phenolic compound, reducing sugar, and phlobotannin. The methanolic extract of the plant was tested for analgesic activity using chemical writhing method (0.6% acetic acid) and antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi bacteria using cup diffusion method. The extract significantly(p<0.05) inhibited in a dose dependent fashion analgesia(nociception) induced by acetic acid as indicated by the reduction in number of writhing movement in mice. Antibacterial activity was observed against S.aureus, S.typhi, E.faecalis, and E.col...

- by Shyam Narayan Mishra
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- Chemistry, Sidha Medicine
- by Fahadul Islam
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The methanol extracts and their pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates of Ophirrhiza mungos, Mussaenda macrophylla, Gmelina philippensis and Synedrella nodiflorawere subjected to assays for membrane stabilizing and thrombolytic activities. The extractives inhibited heat- as well as hypotonic solution-induced haemolysis of human erythrocytes in vitro. The pet-ether soluble fraction of O. mungos, M. macrophylla and S. nodiflora demonstrated 61.16 % & 24.75%, 52.55% & 23.35% and 60.24% & 22.85% inhibition of hemolysis of RBC caused by hypotonic solution and heat, whereas the carbon tetrachloride soluble fraction of G. philippensis showed 49.05% and 21.25% inhibition of hypotonic and heat induced hemolysis of RBC, respectively. Here, acetyl salicylic acid was used as reference standard at 0.10 mg/mL. Among the four plants, the carbon tetrachloride soluble fraction of O. mungos, methanol extract of M. macrophylla, carbon tetrachloride soluble fracti...

- by Mohammad Kaisar
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- Chemistry, Haemolysis, Carbon Tetrachloride, Petroleum ether

The present study was designed to investigate the cytotoxic and thrombolytic activity. Methanolic extract of leaves of B. acuminate was assessed with the Brine shrimp lethality bioassay used to evaluate cytotoxicity. The methanolic extract of B. acuminata was assessed with human blood to evaluate thrombolytic effect. The extract showed remarkable cytotoxic activity, LC50 value of the extract was 15.41μg/ml compared to vincristin sulphate. It also evaluated for thrombolytic agent compared to streptokinase. It has the significant thrombolytic effect which was about 10.058%. These findings demonstrate that the leaves extract of B. acuminata have significance cytotoxic and thrombolytic activities. This plant can be used in further investigation for lead compound isolation and other pharmacological activities.

- by NAIMUL ISLAM
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- Chemistry, Traditional Medicine, In Vitro, Bauhinia
- by Md.Saddam Hussain
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- Pharmacology, Chemistry, Traditional Medicine, In Vitro

An investigation was made to find out the cytotoxic and thrombolytic activity of methanolic extract of leaves of Flamingia macrophylla. In-vitro test to observe thrombolytic and cytotoxic potential was done with the methanolic extract of F. macrophylla in this purpose. Streptokinase was used as positive control and water as negative control in the methodology of thrombolytic testing. In case of cytotoxic activity observation, Brine shrimp lethality bioassay testing method was used, 5% DMSO and brine shrimps were used as test materials, where DMSO as solvent for proper solution. The percent of thrombolysis by the extract showed 43.067 ± 3.0601%, whereas percent of thrombolysis by streptokinase was found 68.582 ±1.7764%. From the cytotxicity study of the crude extract LC50 was found 1.981µg/ml with 95% confidence limit was 1.7299-2.2706 µg/ml. The current study refers the plant leaves as impressive thrombolytic and cytotoxic agent for further laboratory study.

- by KAZI OMAR FARUQ
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- Traditional Medicine, Medicine, Brine shrimp

- by Deepak Basyal
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- Chemistry, Traditional Medicine, MRSA, Phytochemical