Viverridae Research Papers - Academia.edu (original) (raw)

Borneo harbours more endemic carnivores than does any other island in the world except Madagascar, and almost half of the Bornean carnivore species have been classified by The IUCN Red List of Threatened Species as globally threatened.... more

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- Species Distribution Models, Southeast Asia, Carnivora, Felidae

Knowledge of immunodominant regions in major viral antigens is important for rational design of effective vaccines and diagnostic tests. Although there have been many reports of such work done for SARS-CoV, these were mainly focused on the immune responses of humans and mice. In this study, we aim to search for and compare immunodominant regions of the spike (S) and nucleocapsid (N) proteins which are recognized by sera from different animal species, including mouse, rat, rabbit, civet, pig and horse. Twelve overlapping recombinant protein fragments were produced in Escherichia coli, six each for the S and N proteins, which covered the entire coding region of the two proteins. Using a membrane-strip based Western blot approach, the reactivity of each antigen fragment against a panel of animal sera was determined. Immunodominant regions containing linear epitopes, which reacted with sera from all the species tested, were identified for both proteins. The S3 fragment (aa 402-622) and the N4 fragment (aa 220-336) were the most immunodominant among the six S and N fragments, respectively. Antibodies raised against the S3 fragment were able to block the binding of a panel of S-specific monoclonal antibodies (mAb) to SARS-CoV in ELISA, further demonstrating the immunodominance of this region. Based on these findings, one-step competition ELISAs were established which were able to detect SARS-CoV antibodies from human and at least seven different animal species. Considering that a large number of animal species are known to be susceptible to SARS-CoV, these assays will be a useful tool to trace the origin and transmission of SARS-CoV and to minimise the risk of animal-to-human transmission.

- by Jody D Berry
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- Immunology, Immune response, Cell line, Viverridae

The Pleistocene endemic fauna of Cyprus consists mainly of dwarf hippos and elephants, adapted to the insular environment. Among the rare elements of this endemic fauna is a small carnivore, the viverrid Genetta plesictoides, poorly known from scanty material that comes from two localities. Recent excavations in a rockshelter site at the area of Aghia Napa yielded—apart from a rich hippo sample—new skeletal remains (a partial skull and a tibia) of the cypriot genet. The dental morphology shows adaptations towards a ...

- by Socrates Roussiakis
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- Paleontology, Endemism, Cyprus, Carnivora

Background: Rabies is endemic in Sri Lanka, but little is known about the temporal and spatial trends of rabies in this country. Knowing these trends may provide insight into past control efforts and serve as the basis for future control measures. In this study, we analyzed distribution of rabies in humans and animals over a period of 12 years in Sri Lanka.

- by Devika Perera
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- Geography, Sri Lanka, Cats, Biological Sciences

The Viverridae (Mammalia, Carnivora), one of the least studied groups of carnivorans, include two subfamilies of Asian palm civets: Hemigalinae and Paradoxurinae. The relationships between and within these two subfamilies have never been thoroughly tested using an extensive molecular sample set. In this study, we gathered sequences of four genes (two mitochondrial: Cytochrome b and ND2 and two nuclear: b-fibrinogen intron 7 and IRBP exon 1) for eight of the eleven extant species representing these two subfamilies. The results showed that: (1) the Asian palm civets (Hemigalinae and Paradoxurinae) have a single origin and form the sister-group of the (Genettinae + Viverrinae) clade, (2) the Hemigalinae (including the otter civet Cynogale bennettii) are monophyletic, (3) the Paradoxurinae are monophyletic and (4) the small-toothed palm civet (Arctogalidia trivirgata) is an early offshoot within the Paradoxurinae. Using a relaxed molecular clock analysis, the differentiation of the (Hemigalinae + Paradoxurinae) was inferred to occur in the Late Oligocene/Early Miocene.

- by ryan akbar
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- Evolutionary Biology, Genetics, Zoology, Fibrinogen

The Pleistocene endemic fauna of Cyprus consists mainly of dwarf hippos and elephants, adapted to the insular environment. Among the rare elements of this endemic fauna is a small carnivore, the viverrid Genetta plesictoides, poorly known from scanty material that comes from two localities. Recent excavations in a rockshelter site at the area of Aghia Napa yielded —apart from a rich hippo sample— new skeletal remains (a partial skull and a tibia) of the cypriot genet. The dental morphology shows adaptations towards a more carnivorous diet in relation to the extant Genetta genetta.

- by Athanassios Athanassiou
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- Paleontology, Endemism, Cyprus, Carnivora

The first camera-trapping study in the Maliau Basin Conservation Area of Malaysian Borneo, an area containing some of the last intact
rainforest in Southeast Asia, detected 13 small mammalian carnivore species over 2,915 camera-trap-nights, including three newly recorded
for the area: Banded Linsang Prionodon linsang, Masked Palm Civet Paguma larvata and Hose’s Civet Diplogale hosei. Hose’s
Civet, endemic to Borneo, is one of the least known carnivore species in Southeast Asia and possibly the world. Maliau is only the fifth
site where the species has been confirmed in Sabah. Camera-traps—deployed in three habitats: primary (unlogged) dipterocarp forest,
logged dipterocarp forest and tropical heath (kerangas) forest—detected more small carnivore species in primary dipterocarp forest than
in logged forest, despite similar sampling effort; data were too few to allow the estimation of meaningful habitat-specific detection probabilities.
The Malay Civet Viverra tangalunga was the small carnivore species detected most often overall, followed by Leopard Cat
Prionailurus bengalensis and Banded Palm Civet Hemigalus derbyanus. We did not detect Malay Civet or Leopard Cat in logged forest,
yet other studies suggest that they are common in disturbed areas. The paucity of records (only one) of the generally abundant Common
Palm Civet Paradoxurus hermaphroditus is unusual. Ongoing monitoring at this and other sites across Borneo should help elucidate
patterns of small carnivore distribution and richness with respect to natural and anthropogenic variation in forest characteristics.

- by Anthony J Giordano
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- Mustelidae, Viverridae, Borneo, Camera Traps

A dataset of 103 SARS-CoV isolates (101 human patients and 2 palm civets) was investigated on different aspects of genome polymorphism and isolate classification. The number and the distribution of single nucleotide variations (SNVs) and insertions and deletions, with respect to a "profile", were determined and discussed ("profile" being a sequence containing the most represented letter per position). Distribution of substitution categories per codon positions, as well as synonymous and non-synonymous substitutions in coding regions of annotated isolates, was determined, along with amino acid (a.a.) property changes. Similar analysis was performed for the spike (S) protein in all the isolates (55 of them being predicted for the first time). The ratio Ka/Ks confirmed that the S gene was subjected to the Darwinian selection during virus transmission from animals to humans. Isolates from the dataset were classified according to genome polymorphism and genotypes. Gen...

- by Gordana Pavlovic-Lazetic
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- Computational Biology, Studies, Biological Sciences, Phylogeny

Knowledge of the distribution and habitat preferences of a species is of paramount importance when assessing its conservation status. We used accurately recorded occurrence records and ecological niche modelling to predict the distribution of two threatened and poorly known small carnivore species that occur in Southeast Asia, the banded civet (Hemigalus derbyanus) and Hose’s civet (Diplogale hosei), and analysed their spatial niche differentiation for habitat and elevation. We then identified possible anthropogenic threats, and used our modelling predictions to recommend surveying priorities. The predicted distribution of the banded civet was principally in lowland evergreen forest in southern Myanmar/Thailand, Peninsular Malaysia, Sumatra, Borneo and three Mentawai Islands (Siberut, Sipora and South Pagai), and for Hose’s civet in evergreen forest across the higher elevation regions of Borneo. Our niche analyses suggested that there is a tendency for these two species to separate spatially along an elevation gradient: the banded civet is mainly found in lowland areas, whereas Hose’s civet primarily occurs at higher elevations. Our study strongly indicated that these two viverrids are forest-dependent species that may be threatened by forest loss, degradation and fragmentation. Field surveys should be prioritised in areas where each species is predicted to occur and no records currently exist.

- by Anthony J Giordano
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- Zoology, Malaysia, Carnivora, Heart Of Borneo project

The Iberian lynx, (Lynx pardinus), is the most endangered felid in the world. To determine whether sympatric carnivores are reservoirs of pathogens posing a disease risk for the lynx, evidence of exposure to 17 viral, bacterial and protozoan agents was investigated in 176 carnivores comprising 26 free-living lynx, 53 domestic cats, 28 dogs, 33 red foxes (Vulpes vulpes), 24 Egyptian mongooses (Herpestes ichneumon), 10 common genets (Genetta genetta) and 2 Eurasian badgers (Meles meles) in the areas inhabited by the last two populations of Iberian lynx, both in Andalusia (South-Western Spain).

- by alejandro rodriguez
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- Genetics, Epidemiology, Endangered Species, Veterinary

Microbial populations in the gastrointestinal tract contribute to host health and nutrition. Although gut microbial ecology is well studied in livestock and domestic animals, little is known of the endogenous populations inhabiting primates or carnivora. We characterized microbial populations in fecal cultures from gorillas (Gorilla gorilla gorilla), common chimpanzees (Pan troglodytes), Hamadryas baboons (Papio hamadryas) and binturongs (Arctictis binturong) to compare the microbiomes associated with different gastrointestinal morphologies and different omnivorous feeding strategies. Each species was fed a distinct standardized diet for 2 weeks prior to fecal collection. All diets were formulated to reflect the species' feeding strategies in situ. Fresh fecal samples were pooled within species and used to inoculate in vitro batch cultures. Acetate, propionate, butyrate and valerate were measured after 24 h of incubation. Eubacterial DNA was extracted from individual fecal sampl...

- by Erin McKenney
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- Zoology, Phylogeny, Papio hamadryas, Viverridae

The banded civet Hemigalus derbyanus is a small, little-known civet with a geographical range comprising Indonesia (Kalimantan, Sumatra and some associated islands), Malaysia, Brunei, peninsular Myanmar and peninsular Thailand. Habitat... more

In the course of a study on the diet of two Portuguese carnivores, the red fox (Vulpes vulpes Linnaeus) and the common genet (Genetta genetta Linnaeus), 276 ticks were found inside the 940 scats analyzed. Prevalence in samples was 7.6% for both predators. Association of ticks with prey identified on scats, resulted in new data for Portugal on host-tick interactions [e.g. Ixodes acuminatus (Neumann, 1901) and wood mouse] and tick distribution pattern. These unusual findings, besides shedding some light on the host-tick Portuguese assemblage, revealed scats as a new source of biological information.

- by Marina Rodrigues
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- Genetics, Zoology, Nonparametric Statistics, Portugal

Multidirectional comparative chromosome painting was used to investigate the karyotypic relationships among representative species from three Feliformia families of the order Carnivora (Viverridae, Hyaenidae and Felidae). Complete sets of painting probes derived from flow-sorted chromosomes of the domestic dog, American mink, and human were hybridized onto metaphases of the spotted hyena (Crocuta crocuta, 2n = 40) and masked palm civet (Paguma larvata, 2n = 44). Extensive chromosomal conservation is evident in these two species when compared with the cat karyotype, and only a few events of chromosome fusion, fission and inversion differentiate the karyotypes of these Feliformia species. The comparative chromosome painting data have enabled the integration of the hyena and palm civet chromosomes into the previously established comparative map among the domestic cat, domestic dog, American mink and human and improved our understanding on the karyotype phylogeny of Feliformia species.

- by Elena Alkalaeva
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- Genetics, Carnivora, Cats, Dogs
- by Chieko Kai
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- Biology, Japan, Medicine, Phylogeny

The Viverridae (Mammalia, Carnivora), one of the least studied groups of carnivorans, include two subfamilies of Asian palm civets: Hemigalinae and Paradoxurinae. The relationships between and within these two subfamilies have never been thoroughly tested using an extensive molecular sample set. In this study, we gathered sequences of four genes (two mitochondrial: Cytochrome b and ND2 and two nuclear: β-fibrinogen intron 7 and IRBP exon 1) for eight of the eleven extant species representing these two subfamilies. The results showed that: (1) the Asian palm civets (Hemigalinae and Paradoxurinae) have a single origin and form the sister-group of the (Genettinae + Viverrinae) clade, (2) the Hemigalinae (including the otter civet Cynogale bennettii) are monophyletic, (3) the Paradoxurinae are monophyletic and (4) the small-toothed palm civet (Arctogalidia trivirgata) is an early offshoot within the Paradoxurinae. Using a relaxed molecular clock analysis, the differentiation of the (Hemigalinae + Paradoxurinae) was inferred to occur in the Late Oligocene/Early Miocene.

- by Rovie-Ryan Jeffrine J
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- Evolutionary Biology, Genetics, Zoology, Fibrinogen

Bats were recently identified as natural reservoirs of SARS-like coronavirus (SL-CoV) or SARS coronavirus-like virus. These viruses, together with SARS coronaviruses (SARS-CoV) isolated from human and palm civet, form a distinctive cluster within the group 2 coronaviruses of the genus Coronavirus, tentatively named group 2b (G2b). In this study, complete genome sequences of two additional group 2b coronaviruses (G2b-CoVs) were determined from horseshoe bat Rhinolophus ferrumequinum (G2b-CoV Rf1) and Rhinolophus macrotis (G2b-CoV Rm1). The bat G2b-CoV isolates have an identical genome organization and share an overall genome sequence identity of 88-92 % among themselves and between them and the human/civet isolates. The most variable regions are located in the genes encoding nsp3, ORF3a, spike protein and ORF8 when bat and human/civet G2b-CoV isolates are compared. Genetic analysis demonstrated that a diverse G2b-CoV population exists in the bat habitat and has evolved from a common ancestor of SARS-CoV.

- by Guoping ZHAO
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- China, Chiroptera, Biological Sciences, General

- by Ioannis Giaourtsakis
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- Paleontology, Endemism, Cyprus, Carnivora

The exhibits obtained in wildlife offence cases quite often present a challenging situation for the forensic expert. The selection of proper approach for analysis is vital for a successful analysis. A generalised forensic analysis approach should proceed from the use of non-destructive techniques (morphological and microscopic examination) to partially destructive and finally destructive techniques (DNA analysis). The findings of non-destructive techniques may sometime be inconclusive but they definitely help in steering further forensic analysis in a proper direction. We describe a recent case where a very small dried skin piece (b 0.05 mg) with just one small trimmed guard hair (0.4 cm) on it was received for species identification. The single guard hair was examined microscopically to get an indication of the type of species. We also describe the extraction procedure with a lower amount of sample, using an automated extraction method (Qiagen Biorobot EZ1 ®) and PCR amplification of three mitochondrial genes (16s rRNA, 12s rRNA and cytochrome b) for species identification. Microscopic examination of the single hair indicated a viverrid species but the initial DNA analysis with 16s rRNA (through NCBI BLAST) showed the highest homology (93%) with a hyaenid species (Hyaena hyaena). However, further DNA analysis based on 12s rRNA and cytochrome b gene proved that the species was indeed a viverrid i.e. Viverricula indica (small Indian civet). The highest homology shown with a Hyaenid species by the 16s rRNA sequence from the case sample was due to lack of a 16s rRNA sequence for Viverricula indica in the NCBI data base. The case highlights the importance of morphological and microscopic examinations in wildlife offence cases. With respect to DNA extraction technology we found that automatic extraction method of Biorobot EZ1 ® (Qiagen) is quite useful with less amount of sample (much below recommended amount).

- by Vivek Sahajpal
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- Microscopy, Forensic Genetics, Multidisciplinary, India
- by Devika Perera
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- Molecular Evolution, Medical Microbiology, Sri Lanka, Endangered Species

Evidence in support of a novel lyssavirus was obtained from brain samples of an African civet in Tanzania. Results of phylogenetic analysis of nucleoprotein gene sequences from representative Lyssavirus species and this novel lyssavirus... more

- by Chanasa Mpelumbe-Ngeleja
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- Medical Microbiology, Tanzania, Emerging Infectious Diseases, Phylogeny

Severe acute respiratory syndrome (SARS) emerged in 2002-3 in southern China. The origin of its etiological agent, the SARS coronavirus (SARS-CoV), remains elusive. Here we report that species of bats are a natural host of coronaviruses closely related to those responsible for the SARS outbreak. These viruses, termed SARS-like coronaviruses (SL-CoV), display greater genetic variation than SARS-CoV isolated from humans or civets. The human and civet isolates of SARS-CoV nestle phylogenetically within the spectrum of SL-CoVs, indicating that the virus responsible for the SARS outbreak was a member of this coronavirus group.

- by Hume Field
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- Science, China, Chiroptera, Multidisciplinary

Vaccinesagainstrotavirusesarenowavailableinnumerouscountries,includingTurkey.Asthevaccinesmayshowvariousefficienciesagainstdifferenttypespecificitiesandroutinevaccinationininfantsmightresultinselectionandimmuneescapeofwild-type rotavirus strains, strain surveillance has been initiated before and during the vaccine introduction. We aimed to provide corresponding information on local strain prevalence in Anatolia, mid-western Turkey during the introduction of rotavirus vaccines. Stool samples positive for group A rotavirus by commercial enzyme immunoassay were subjected to reverse transcription-polymerase chain reaction basedgenotypingoftheoutercapsidantigens,VP7andVP4,determiningGandPtypespecificitiesrespectively.Among36fullyand 5partiallytypeablestrainswedetectedgenotypeG1,G2,andG9VP7specificitiesandgenotypeP[4],P[6]andP[8]VP4specificities in5individualand4mixedcombinations.ThemostcommonstrainwasG2P[4](n=17),followedbyG9P[8](n=9).Otherstrains wereG1P[8](n=2),G2P[8](n=2),G1+2P[8](n=2),G9P[4](n=1),G2+9P[8](n=1),G4+9P[6](n=1),andG2P[4+8](n=1). Partiallytypedstrainsincluded2G1P[NT]and3G2P[NT]strains.Ourdatamayhelpdetermineabaselineoftherotavirusgenotype prevalence in Turkey and see if changes in the incidence of individual strains will be observed after routine use of vaccine. © Versita Sp. z o.o.

- by Teoman Apan
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- Microbiology, Veterinary Microbiology, Japan, Veterinary

Leishmania spp. infection was investigated in tissue samples of wild carnivores from the Spanish Basque Country (BC), by PCR and DNA sequencing. The region is at the northern periphery of Leishmania infantum endemic Iberian Peninsula and infection in the dog (reservoir) or other species has not been previously reported. Leishmania kinetoplast DNA was detected by real-time PCR (rtPCR) in 28% (44/156) of animals. Specifically, in 26% of Eurasian badgers (n = 53), 29% of foxes (n = 48), 29% of stone martens (n = 21) and in 25-50% of less numerous species including genets, wild cats, pole cats, European mink and weasels. Infected animals particularly badgers, were most prevalent in the southernmost province of the BC (Araba) in areas dominated by arable land. Subsequent amplification and sequencing of a fragment of the rRNA internal transcribed spacer 2 (ITS2) from a subset of rtPCR positives samples confirmed the species as L. infantum, showing a high sequence homogeneity with ITS2 sequences of L. infantum from dogs and humans from southern Spain. In summary, this study reports for the first time L. infantum infection in wild carnivores from the BC including in stone martens, pole cats and minks in which infection has not been previously described. It supports the need to study infection in dogs and people in this region and is an example of the value of infection surveillance in wildlife to assess potential risks in the domestic environment and their role in spreading infections in non-endemic areas.

- by Marta Barral and +2
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- Spain, Preventive Veterinary Medicine, Mustelidae, Sequence alignment

Page 1. 628 But how well do these new observations fit with the predictions of global climate models (GCMs)? The positive feedbacks on global warming stemming from the reduction in snow cover are already included within GCMs. ...

- by Andrew Dobson
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- Science, Chiroptera, Multidisciplinary, Viverridae

Massive numbers of palm civets were culled to remove sources for the reemergence of severe acute respiratory syndrome (SARS) in Guangdong Province, China, in January 2004, following SARS coronavirus detection in market animals. The virus... more

- by Zheng Han
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- Molecular Evolution, Virology, China, Biological Sciences

Currently, there are no complete parameters established for serum biochemistry and hematology for the determination of health status of rescued common palm civets (Paradoxurus hermaphroditus). In this study, blood samples were obtained from 18 adults and 15 juvenile civets caught on Singapore Main Island. Significant age-related differences (P<0.05) were noted in the hemoglobin, erythrocyte count, packed cell volume (PCV), total serum protein and globulin concentration in the adult civets showing higher values compared with the juvenile civets. The mean corpuscular volume (MCV), the alkaline phosphatase (ALP) and the phosphorus concentrations were significantly higher (P<0.05) in juveniles compared with adult civets.

- by Lucy Spelman
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- Zoology, Phosphorus, Viverridae, North Carolina
- by Christine Drea
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- Reproduction, Diet, Pheromones, Multidisciplinary

Ten wild masked palm civets infected with canine distemper virus (CDV), captured in Japan from 2005 to 2007, were histopathologically and phylogenetically analyzed. Phylogenetic analysis based on the amino acid sequences of the H protein of two CDV isolates from masked palm civets revealed that the two isolates were classified into the clade of recent isolates in Japan. Histopathologically marked lesions of virus encephalitis were present in the brain, whereas gastrointestinal lesions were absent or at a mild degree. The distribution of the lesions resembles that of recent CDV cases in dogs. Therefore, recent CDV infections in masked palm civets could be caused by recently prevalent CDV in dogs. The possibility of the masked palm civet as a spreader of CDV among wildlife is also discussed. #

- by Tokuma Yanai
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- Japan, Infectious Diseases, Biological Sciences, Phylogeny

- by Vivek Fellner
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- Zoology, Phylogeny, Papio hamadryas, Viverridae

- by Marta Barral and +1
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- Genetics, Veterinary, Toxoplasma gondii, Spain