Amplicon (original) (raw)
في علم الأحياء الجزيئي، يُعرف الامبليكون على أنه جزءً من الحمض النووي أو الحمض النووي الريبي الذي ينتج من عمليات التضاعف. ويمكن تكوينه بطريقة صناعية، باستخدام طرق مختلفة من ضمنها في ذلك تفاعلات سلسلة البوليميريز (PCR) أو تفاعلات سلسلة ligase (LCR)، أو بشكل طبيعي من خلال ازدواج الجينات. في هذا السياق، يشير التضخيم إلى إنتاج نسخة واحدة أو أكثر من جزء جيني أو تسلسل مستهدف، وتحديداً الأمبليكون. لأنه يشير إلى منتج تفاعل التضخيم، يستخدم amplicon بالتبادل مع المصطلحات المخبرية الشائعة، مثل «منتج PCR».
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| dbo:abstract | في علم الأحياء الجزيئي، يُعرف الامبليكون على أنه جزءً من الحمض النووي أو الحمض النووي الريبي الذي ينتج من عمليات التضاعف. ويمكن تكوينه بطريقة صناعية، باستخدام طرق مختلفة من ضمنها في ذلك تفاعلات سلسلة البوليميريز (PCR) أو تفاعلات سلسلة ligase (LCR)، أو بشكل طبيعي من خلال ازدواج الجينات. في هذا السياق، يشير التضخيم إلى إنتاج نسخة واحدة أو أكثر من جزء جيني أو تسلسل مستهدف، وتحديداً الأمبليكون. لأنه يشير إلى منتج تفاعل التضخيم، يستخدم amplicon بالتبادل مع المصطلحات المخبرية الشائعة، مثل «منتج PCR». (ar) Amplicon bezeichnet in der Biochemie eine DNA-Sequenz oder RNA-Sequenz, die per Amplifikation vervielfältigt wird. (de) In molecular biology, an amplicon is a piece of DNA or RNA that is the source and/or product of amplification or replication events. It can be formed artificially, using various methods including polymerase chain reactions (PCR) or ligase chain reactions (LCR), or naturally through gene duplication. In this context, amplification refers to the production of one or more copies of a genetic fragment or target sequence, specifically the amplicon. As it refers to the product of an amplification reaction, amplicon is used interchangeably with common laboratory terms, such as "PCR product." Artificial amplification is used in research, forensics, and medicine for purposes that include detection and quantification of infectious agents, identification of human remains, and extracting genotypes from human hair. Natural gene duplication plays a major role in evolution. It is also implicated in several forms of human cancer including primary mediastinal B cell lymphoma and Hodgkin's lymphoma. In this context the term amplicon can refer both to a section of chromosomal DNA that has been excised, amplified, and reinserted elsewhere in the genome, and to a fragment of extrachromosomal DNA known as a double minute, each of which can be composed of one or more genes. Amplification of the genes encoded by these amplicons generally increases transcription of those genes and ultimately the volume of associated proteins. (en) Un amplicon est un fragment d'ADN amplifié par PCR (réaction en chaîne par polymérase). (fr) In biologia molecolare, un amplicone è un frammento di DNA o RNA che è la fonte e/o il prodotto di reazioni di amplificazione o di replicazione. Un amplicone può essere sintetizzato artificialmente, utilizzando vari metodi tra i quali le reazioni a catena della polimerasi (PCR), le reazioni a catena della ligasi (LCR) oppure sintetizzato naturalmente attraverso la replicazione genica. In questo contesto, l'amplificazione si riferisce alla produzione di una o più copie di un frammento genetico o sequenza bersaglio, in particolare l'amplicone. Poiché si riferisce al prodotto di una reazione di amplificazione, l'amplicone è usato in modo intercambiabile con termini di laboratorio comuni, come "prodotto di PCR" o "sequenza target". L'amplificazione artificiale è utilizzata nella ricerca, nelle scienze forensi, in medicina, per scopi che includono il rilevamento e la quantificazione di agenti infettivi, l'identificazione di resti umani, e l'estrazione di materiale genetico da capelli o peli umani. La duplicazione genica naturale gioca un ruolo importante nell'evoluzione. È implicata anche in diverse forme di cancro umano tra cui il linfoma primitivo delle cellule B del mediastino e il linfoma di Hodgkin. In questo contesto il termine amplicone può riferirsi sia a una sezione di DNA cromosomico che è stata asportata, amplificata e reinserita altrove nel genoma, sia a un frammento di DNA extracromosomiale noto come , ciascuno dei quali può essere composto da uno o più geni. L'amplificazione dei geni codificati da questi ampliconi generalmente aumenta la trascrizione di quei geni e, di conseguenza, il volume delle proteine per le quali codificano. (it) Amplikon – fragment DNA powstały w naturalnym procesie duplikacji genów lub podczas sztucznej amplifikacji w reakcji PCR lub LCR. (pl) |
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| rdfs:comment | في علم الأحياء الجزيئي، يُعرف الامبليكون على أنه جزءً من الحمض النووي أو الحمض النووي الريبي الذي ينتج من عمليات التضاعف. ويمكن تكوينه بطريقة صناعية، باستخدام طرق مختلفة من ضمنها في ذلك تفاعلات سلسلة البوليميريز (PCR) أو تفاعلات سلسلة ligase (LCR)، أو بشكل طبيعي من خلال ازدواج الجينات. في هذا السياق، يشير التضخيم إلى إنتاج نسخة واحدة أو أكثر من جزء جيني أو تسلسل مستهدف، وتحديداً الأمبليكون. لأنه يشير إلى منتج تفاعل التضخيم، يستخدم amplicon بالتبادل مع المصطلحات المخبرية الشائعة، مثل «منتج PCR». (ar) Amplicon bezeichnet in der Biochemie eine DNA-Sequenz oder RNA-Sequenz, die per Amplifikation vervielfältigt wird. (de) Un amplicon est un fragment d'ADN amplifié par PCR (réaction en chaîne par polymérase). (fr) Amplikon – fragment DNA powstały w naturalnym procesie duplikacji genów lub podczas sztucznej amplifikacji w reakcji PCR lub LCR. (pl) In molecular biology, an amplicon is a piece of DNA or RNA that is the source and/or product of amplification or replication events. It can be formed artificially, using various methods including polymerase chain reactions (PCR) or ligase chain reactions (LCR), or naturally through gene duplication. In this context, amplification refers to the production of one or more copies of a genetic fragment or target sequence, specifically the amplicon. As it refers to the product of an amplification reaction, amplicon is used interchangeably with common laboratory terms, such as "PCR product." (en) In biologia molecolare, un amplicone è un frammento di DNA o RNA che è la fonte e/o il prodotto di reazioni di amplificazione o di replicazione. Un amplicone può essere sintetizzato artificialmente, utilizzando vari metodi tra i quali le reazioni a catena della polimerasi (PCR), le reazioni a catena della ligasi (LCR) oppure sintetizzato naturalmente attraverso la replicazione genica. In questo contesto, l'amplificazione si riferisce alla produzione di una o più copie di un frammento genetico o sequenza bersaglio, in particolare l'amplicone. Poiché si riferisce al prodotto di una reazione di amplificazione, l'amplicone è usato in modo intercambiabile con termini di laboratorio comuni, come "prodotto di PCR" o "sequenza target". (it) |
| rdfs:label | أمبليكون (ar) Amplicon (de) Amplicon (en) Amplicon (fr) Amplicone (it) Amplikon (pl) |
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