Setting the stage: host invasion by HIV (original) (raw)

Key Points

Abstract

For more than two decades, HIV has infected millions of people worldwide each year through mucosal transmission. Our knowledge of how HIV secures a foothold at both the molecular and cellular levels has been expanded by recent investigations that have applied new technologies and used improved techniques to isolate ex vivo human tissue and generate in vitro cellular models, as well as more relevant in vivo animal challenge systems. Here, we review the current concepts of the immediate events that follow viral exposure at genital mucosal sites where most documented transmissions occur. Furthermore, we discuss the gaps in our knowledge that are relevant to future studies, which will shape strategies for effective HIV prevention.

Similar content being viewed by others

Main

HIV vaccines and microbicides hold promise for preventing the acquisition of HIV-1 and HIV-2, the two viruses that cause AIDS, but the success of designing such agents needs a clear understanding of where HIV first encounters its target cells — primarily T cells, macrophages and dendritic cells (DCs) — and how it gains entry at various sites to eventually establish infection. HIV infection has rapidly spread since the early 1980s to become an epidemic disease (see the UNAIDS/WHO AIDS epidemic update) that is largely maintained by sexual transmission through the lower genital and rectal mucosa (Fig. 1, Table 1). Here, we have endeavoured to assemble the current knowledge on the acquisition of HIV at mucosal sites, confining our discussion to the lower genital mucosa. We clearly recognize that other sites of entry, such as the blood, placenta and gastrointestinal mucosa (Box 1), are also important for HIV acquisition but these are beyond the scope of this Review.

Figure 1: HIV invasion sites.

figure 1

a | In women, viral invasion occurs mostly through the non-keratinized squamous epithelium of the vagina and ectocervix, as well as through the single-layer columnar epithelium of the endocervix. The endocervical canal is filled with mucus, providing a barrier against the ascent of pathogens. However, ovulation is accompanied by hydration and alkalinization of the mucus plug, possibly decreasing its barrier function. Infection in women can also ensue when HIV-1 invades the single-layer columnar epithelium of the rectum following receptive anal intercourse. b | In men, viral invasion occurs most frequently through the inner foreskin and the penile urethra as a consequence of penile–vaginal or penile–anal intercourse. Thinly stratified columnar epithelial cells line most of the urethra except for the fossa navicularis near the external meatus (exit hole), which is covered by non-keratinized squamous epithelium. The glans penis and the outer foreskin are protected by keratinized squamous epithelium, which provides a strong mechanical barrier against HIV invasion. By contrast, a thin and poorly keratinized squamous epithelium covers the inner foreskin, rendering this site vulnerable to HIV invasion. Men are also infected by viral invasion through the rectum.

Full size image

Table 1 Contribution of HIV invasion sites to global HIV infections*

Full size table

Many studies have provided insights into certain aspects of HIV and simian immunodeficiency virus (SIV) mucosal entry by carrying out detailed examinations of the relevant tissues and target cells following their in vivo, ex vivo or in vitro exposure to the virus. This Review discusses experimental systems that use the same mucosal source to avoid confusion and the inconsistencies that often emerge when findings from one system are extrapolated to those of another. Where appropriate, we have emphasized the benefits and limitations of the experimental approaches, important considerations in the interpretation of findings and their relevance for future studies.

HIV invasion in the female genital tract

Anatomical sites. An estimated 30–40% of all new HIV-1 infections in women occur through vaginal intercourse, which carries a lower HIV transmission probability per exposure event than anal intercourse or parenteral inoculation (Table 1). Although HIV-1 can infect the vaginal, ectocervical and endocervical mucosa (Fig. 1), the relative contribution of each site to the establishment of the initial infection is not known. The multilayered squamous epithelium that covers the vagina and ectocervix, when intact, provides better mechanical protection against HIV invasion than the single-layer columnar epithelium that lines the endocervix. However, the greater surface area of the vaginal wall and ectocervix, which often exceeds 15 times that of the endocervix, provides more potential access sites for HIV entry, particularly when breaches occur in the epithelial-cell layer. HIV or SIV can establish an initial infection solely through invasion of the vaginal mucosa, as shown in women who lack a uterus at birth1 and in female macaques after surgical removal of the uterus2. In fact, selective transmission of HIV through the vaginal mucosa rather than the cervix may commonly occur, as suggested by a recent large, randomized, controlled, prevention clinical trial in African women. In this study, no significant reduction in HIV-1 acquisition occurred in women using a diaphragm compared with the control group3. However, the observed potential benefit of blocking HIV-1 exposure to the cervix may have been undermined because the sexual partners of the women in the group using a diaphragm reported lower condom use than those in the control group.

The region where the ectocervix transforms into the endocervix (Fig. 1) can have enriched CD4+ T-cell populations and therefore may be a particularly susceptible site for HIV entry. Whether HIV can cross the endocervical mucus plug, reach the uterine cavity and invade through the mucosa of the upper genital tract has not been well examined. In principle, uterine tissue is susceptible to infection if directly inoculated with HIV4. Uterine simian–HIV (SHIV) infection has been shown in one monkey following vaginal inoculation two days earlier, an interval that is probably too short for stromal or lymphatic spread from the lower genital tract5. This indicated that ascent of the virus through the endocervical mucus plug may be possible, but this observation has not been confirmed in humans. Conceivably, the upper mucosal tract may become more vulnerable to HIV-1 penetration during ovulation, a period when rising oestrogen levels alter the endocervical mucus making its consistency less viscous and more alkaline.

HIV entry through the female genital epithelium. Both free and cell-associated HIV and SIV virions can establish mucosal infection (Fig. 2)6,7,8,9,10. This has been shown directly in vivo in female macaques11 and in mice12, and indirectly in humans through genetic sequence comparisons of viral isolates from acutely infected women with those from seminal leukocytes (cell-associated virions) and plasma from their infected source partners13. Ex vivo studies using human cervical explants have also confirmed transmission of cell-free and cell-associated HIV-1 (Refs 14, 15). Initially, cervical mucus can trap infected seminal cells or free virions16,17. Conceivably, this could facilitate viral transmission by prolonging contact time of the virions with the mucosa. However, while immobilized, the virions may also become more susceptible to innate antiviral substances.

Figure 2: Pathways of HIV invasion in the mucosa of the vagina and uterine ectocervix.

figure 2

The human vagina and ectocervix are covered by non-keratinized squamous epithelium. Shearing during sexual intercourse can lead to physical abrasions of the epithelium, in particular in microanatomical regions where the stromal papillae, which are enriched with stromal dendritic cells (DCs), reach close to the luminal surface of the mucosa. Monocytic precursor cells differentiate on arrival either into macrophages or DCs, and DCs may differentiate further into subsets. Three stromal DC subsets have been identified in human skin, distinguished by blood DC antigen 1 (BDCA1), CD1 and CD14 expression patterns64, but their presence and susceptibility to HIV have not been determined in the mucosa. Infected donor cells and free virions may migrate along the abrasion and directly contact various target cells in the mucosal epithelium and stroma. Resident mucosal leukocytes such as DCs and T cells tend to cluster in these regions, creating susceptible foci for infection. Characteristic phenotypic cell receptors and receptors relevant for HIV binding and infection are shown on the top of the figure (A). The possible pathways of HIV penetration are summarized in B. a | Free HIV virions or HIV-infected donor cells are trapped in mucus, resulting in penetration of the free virions into gaps between epithelial cells or attachment of HIV-infected donor cells to the luminal surface of the mucosa and secretion of virions on contact. The virions are then captured and internalized into endocytic compartments by Langerhans cells that reside within the epithelium. b | HIV can also fuse with the surface of intraepithelial CD4+ T cells, followed by productive infection of these cells. c | Infected donor cells or free virions can immigrate along physical abrasions of the epithelium into the mucosal stroma. There they are taken up by lymphatic or venous microvessels and transported to local lymph nodes or into the blood circulation, respectively, or they make contact with stromal DCs, T cells and macrophages. d | Virions can transcytose through epithelial cells near or within the basal layer of the squamous epithelium (see also Figure 3), productively infect basal epithelial cells, be internalized into endocytic compartments, or penetrate between epithelial cells. e | Once within the stroma, virions can productively infect stromal DCs or be internalized into the endocytic compartments of DCs and pass from the stromal DCs to CD4+ T cells across an infectious synapse (see also Figure 4) where massive productive infection of CD4+ T cells ensues. In addition, virions can productively infect resting mucosal CD4+ memory T cells in the stroma and possibly stromal macrophages. f | Productively infected CD4+ T cells and stromal DCs, and stromal DCs or intraepithelial LCs harboring virions in endocytic compartments, can emigrate into the submucosa and the draining lymphatic and venous microvessels. CCR5, CC-chemokine receptor 5; DC-SIGN, dendritic-cell-specific ICAM3-grabbing non-integrin.

Full size image

HIV virions that are initially free, or those that are released from infected donor seminal cells, interact with epithelial cells and traverse the epithelium through several pathways, including transcytosis, endocytosis and subsequent exocytosis, by causing productive infection, or merely by penetrating through the gaps between epithelial cells (Fig. 2). The understanding of these events has been hindered by inconsistent findings, and these are largely a result of the use in experimental systems of epithelial-cell types derived from different anatomical sites, as well as primary and immortalized cell lines. Several reports have shown that HIV-1 binds to and enters epithelial cells from the lower female genital tract18,19,20. Transcytosis has been shown to occur in cell lines and also in primary cells, but this has not been definitively shown in intact tissue. On release from the epithelial cells, the virions readily infect susceptible leukocytes21,22. Interestingly, cell-associated virions secreted from infected seminal leukocytes appear markedly more efficient at transcytosis than cell-free virions7,10,21. It has been reported that virions can also productively infect the cervical epithelial cells themselves6, although this remains controversial18,23. Conceivably, HIV-1 can also be transported through the cervicovaginal epithelium to the draining lymphatics by donor lymphocytes and macrophages, as has been suggested in mouse studies6,24.

Our ex vivo experiments using sheets of isolated vaginal epithelium, devoid of mucosal stroma, confirmed that HIV-1 virions are sequestered in endocytic compartments and in the cytosol of epithelial cells (Fig. 3). Interestingly, although the experimental conditions permitted HIV-1 access to both the luminal and basal sides of the epithelium, the virions were detected exclusively in the basal and suprabasal epithelial cells (F.H., P. Sakchalathorn and M.J.M., unpublished observations). This suggests that initially, rather than entering and traversing superficial epithelial cells in the vagina and ectocervix, HIV-1 probably disperses through the narrow gaps between them16, as depicted in Fig. 2. This route might then permit HIV-1 to directly contact and infect intraepithelial Langerhans cells (LCs) and CD4+ T cells25 (see later), or it might allow HIV-1 to reach suprabasal or basal epithelial cells that are more susceptible to viral sequestration and transcytosis. Importantly, components of human semen, most notably amyloid fibrils that form from naturally occurring fragments of seminal prostatic acidic phosphatase, can capture virions and promote their attachment to epithelial cells and leukocytes, thus increasing infectivity26.

Figure 3: HIV-1 transcytosis in situ in the vaginal epithelium.

figure 3

Electron micrograph showing a vaginal epithelial cell located one layer above the basal cell layer and containing intact cytoplasmic virions following 24 hours of infection with HIV-1BaL (a procedure performed on vaginal epithelial sheets, as previously described25) (a). As shown in the higher power insets, the virions can be seen on both the apical (b) and basal (c) sides of the nucleus, signifying transcytosis. Desmosomes and keratin fibres, distinguishing features of the epithelial cell, can also be seen (c).

Full size image

Several proteins expressed on the surface of epithelial cells may mediate the attachment of HIV-1. Two cell-surface glycosphingolipids, sulphated lactosylceramide expressed by vaginal epithelial cells27 and galactosylceramide expressed by ectocervical epithelial cells18,28, bind HIV-1 gp120 and foster transcytosis21. Interactions of HIV-1 gp120 with transmembrane heparan sulphate proteoglycans (syndecans) expressed by genital epithelial cells can also contribute to HIV-1 attachment and entry19,22. Recently, gp340, a splice variant of salivary agglutinin that is expressed by cervical and vaginal epithelial cells, was shown to specifically bind to the HIV envelope protein and to enhance the passage of HIV through the epithelium giving it access to susceptible leukocytes29. One research group found that the β1 subunit of integrins expressed by cervical epithelial cells from some explants, but not all, bound virions that were presumably coated with fibronectin, which is abundant in human semen16. Detection of HIV-1 chemokine co-receptor expression has been inconsistent: one study did not detect the expression of either CC-chemokine receptor 5 (CCR5) or CXC-chemokine receptor 4 (CXCR4) by cervical epithelial cells18, another reported the expression of CXCR4 by these cells20, whereas another reported the exclusive expression of CCR5 (Ref. 28).

Regardless of the mode, the penetration of virus through the cervicovaginal epithelium in vivo occurs rapidly within 30–60 minutes of exposure, as shown in SIV-infected macaques30. Once within the epithelium, HIV encounters CD4+ T cells as well as LCs. LCs have dendrites that extend and retract through the intercellular spaces31, and which can even reach up to the surface of the epithelium32, where HIV can bind directly to them (T. Hope, personal communication). Based on observations of gut DCs33,34, this could also be particularly true for DCs that are located just beneath the endocervical columnar epithelium. However, direct sampling of luminal pathogens by endocervical DCs or vaginal LCs, which could be exploited by HIV to bypass the epithelial-cell barrier, has not yet been formally demonstrated.

Mechanical micro-abrasions of the mucosal surface induced by intercourse may also allow HIV to directly access target cells, such as DCs, T cells and macrophages, at the basal epithelium and the underlying stroma35. Areas above the stromal papillae, where the epithelium is relatively thin and where LCs on the epithelial-cell side (F.H., L. Ballweber and M.J.M., unpublished observations) and T cells and macrophages on the stromal side28 congregate, appear particularly vulnerable to viral invasion. Consistent with this notion, in vivo simian immunodeficiency virus (SIV) infection of the genital mucosa of macaques is initially established in a highly focal manner, and continuous seeding from this nidus of infection is crucial for establishing systemic infection17. Similarly, chemical micro-abrasions from the use of certain topical microbicides and micro-abrasions due to genital ulcers caused by sexually transmitted diseases (for example, syphilis, chancroid and those caused by herpes simplex virus) are also likely to result in the exposure of vulnerable target cells in the basal epithelium and stroma36.

Importance of cervicovaginal LCs in HIV invasion. For many years, HIV-1 invasion of the lower genital tract has been assumed to occur through the internalization of HIV-1 by LCs. This view was supported by evidence that skin LCs are susceptible to infection by HIV-1 (Refs 37,38,39,40) and that genital mucosal LCs harbour SIV virions within 24 hours of intravaginal inoculation of macaques30. However, soon after ex vivo organ culture, LCs migrate out of the epithelium14,23,41,42,43; therefore, examination of LC infection specifically within the human vaginal epithelium has been technically difficult. For example, one landmark study demonstrated that after exposure of complete cervical mucosa from humans to HIV-1, emigrating DCs had efficiently captured HIV and were capable of transmitting the virus in trans42. However, it was not possible to determine whether the cells originated from the epithelium as LCs or from the underlying stroma as DCs. More recently, we resolved this issue by preparing sheets of vaginal epithelium separated from the underlying stroma, and observed that vaginal LCs efficiently internalized HIV-1 into their cytoplasmic compartments25. As LCs exit the epithelium at the basal side, they transport intact virions, thereby enabling the infection to spread beyond the site of viral entry (Fig. 2).

However, it is not clear if LCs in the cervicovaginal epithelium can produce and release new HIV-1 virions. HIV receptors expressed by these LCs include CD4, CCR5 and the C-type lectin receptor langerin (also known as CD207), but not CXCR4 and DC-specific ICAM3 (intercellular adhesion molecule 3)-grabbing non-integrin (DC-SIGN; also known as CD209)25,44,45,46,47. Antibodies that bind CD4 and CCR5 partially block the uptake of R5-tropic HIV-1 by LCs, but blocking the binding of HIV-1 to C-type lectin receptor with mannan, a mannose polymer, had little effect on uptake25. Although low-level CD4- and CCR5-mediated productive infection of LCs by HIV-1 in human skin explants has been shown39,40,48, we were unable to confirm this finding in our imaging studies of vaginal LCs25. Therefore, if de novo production of virions occurs, it appears relatively inefficient in contrast to the high capacity of vaginal LCs to endocytose HIV-1. Nevertheless, even low levels of productive HIV-1 infection of cutaneous DCs and LCs lead to profound viral replication in co-cultured T cells40,48,49. Therefore, future investigations must conclusively determine if LCs in cervicovaginal epithelium can support productive HIV infection in vivo and if this property is required for the passage of the virus to T cells, as has been reported for other types of DC50,51,52,53.

The relative inefficiency of mannan in blocking the binding and endocytosis of HIV-1 by vaginal LCs was surprising25 because C-type lectin receptors, which recognize mannose-containing carbohydrate structures, mediate viral entry in other types of DCs46. However, HIV-1 can bind DC subsets independent of C-type lectin receptors and CCR5 (Refs 54,55,56). So, although HIV virions can efficiently bind to langerin expressed by epidermal LCs for their entry into these cells57, they appear to largely bypass binding to langerin expressed by vaginal LCs in favour of alternative endocytic routes. This distinction may be highly relevant for mucosal HIV transmission. Langerin expressed by epidermal LCs can direct HIV-1 to Birbeck granules for degradation57. By contrast, by gaining entry to the vaginal LCs independently of langerin, HIV may survive by reaching endocytic compartments, such as early phagosomes, where antigens are preserved for cross-presentation58. This is consistent with our observations that intact virions were still present in LCs that had migrated out of the vaginal epithelium 60 hours after viral challenge25. Therefore, it appears that HIV-1 enters vaginal LCs through a different route than when it enters skin LCs, and this results in a distinct fate of the endocytosed virions. More detailed studies are now warranted to uncover which endocytic pathways HIV uses in vaginal LCs, and how this can be harnessed therapeutically.

Infection of DCs in the cervicovaginal stroma. Unlike genital LCs, stromal DCs express both DC-SIGN46,47 and CCR5 (Refs 59, 60) and have been implicated in SIV and HIV infection, but their exact role in mucosal transmission is not clear. In situ studies in the human explant model have failed to identify infected DCs in the cervicovaginal stroma14,23,41,43. By contrast, SIV-infected DCs were present in the lamina propria of the cervicovaginal mucosa of macaques shortly after intravaginal SIV challenge30,61, as well as in chronically infected animals62. Likewise, HIV-infected DCs were identified in tissue biopsies of the vaginal stroma of asymptomatic HIV-1-infected women63.

The failure to identify infection of stromal DCs in the human explant models might be attributable to the relatively low sensitivity of the detection methods used and the migration of stromal DCs from the tissue, which may drastically decrease the number of infected cells in situ over time. Indeed, when DCs were harvested from the culture supernatants of human cervical explants that were challenged with HIV-1, significant in trans infectivity was detected42 and massive budding of virions was observed among emigrant DCs 5 days after virus exposure60 (Fig. 4). However, it could not be determined if the original source of DCs was from the epithelium proper or from the stromal tissue. In addition, inferring the initial susceptibility of DCs while confined to the mucosal stroma, based on findings from emigrated DCs that undergo phenotypic changes as they exit the mucosa, may be less reliable.

Figure 4: The significance of DC–T-cell interactions for HIV-1 transmission.

figure 4

a | Pathways of HIV-1 passage between dendritic cells (DCs) and T cells. DCs can store HIV-1 in three forms for eventual infection of CD4+ T cells. (1) HIV can be stored as endocytosed intact virions in multivesicular bodies, following endocytic entry via C-type lectins such as DC-specific ICAM3-grabbing non-integrin (DC-SIGN; also known as CD209). (2) HIV-1 can also be stored as an integrated provirus following entry by CD4- and co-receptor-mediated fusion, which leads to productive infection of DCs. (3) HIV can be stored as surface-bound intact virions by C-type lectins, such as DC-SIGN. Passage of HIV-1 from DCs to CD4+ T cells occurs most effectively across an infectious synapse (yellow shading), formed by a concentration of HIV-1 virions on the DC side and of HIV receptors such as CD4 and CC-chemokine receptor 5 (CCR5) on the T-cell side. HIV is released into the infectious synapse either by exocytosis of stored virions from multivesicular bodies or by budding of newly formed virions following active viral replication. Virions trapped to surface receptors such as the C-type lectin DC-SIGN can also accumulate in the infectious synapse. Migration of HIV toward the T cell may be further enhanced by 'surfing' of virions along the outer surface of filopodia or cytonemes that are extended from the T cell toward the DC. Coupling of virions to exosomes as they are being released from multivesicular bodies also increases their infectivity. Exosome-associated virions are likely to be transmitted to CD4+ T cells through membrane binding and fusion, either within the infectious synapse or over longer distances. In parallel with transmission of virus from the DC to the CD4+ T cell, the DC also presents antigenic peptides through MHC class II molecules to the T-cell receptor (TCR). During peptide recognition, additional receptor–ligand pairs that are important for T-cell stimulation accumulate in this region and form an immunological synapse (orange shading). Signals delivered through the immunological synapse lead to T-cell activation, which ultimately causes transcription factors such as nuclear factor-κB (NF-κB) and nuclear factor of activated T cells (NFAT) to translocate into the nucleus of the T cell. There, they bind to the enhancer region of the viral long terminal repeat (LTR) and activate viral gene transcription, driving HIV-1 replication. b | Example of infectious-synapse formation between DCs and T cells in the human vagina (images reproduced with permission from Ref. 60 © (1999) American Society For Microbiology). HIV-1 buds from the surface of the productively infected DC toward the contact zone between the DC and the two T cells. Viral budding is also seen along other areas of the DC surface. The DC contains the typical veiled nucleus as well as multiple large mitochondria, and one large cytoplasmic process is formed at the top right. ICAM, intercellular adhesion molecule.

Full size image

Therefore, stromal DCs exhibit different HIV-1 receptor expression patterns compared with LCs, potentially permitting different HIV-1 entry pathways. Much still remains to be learned about stromal DCs in the human genital mucosa in general, whether different subsets exist similar to those found in skin dermis64, and the interaction of these DC subsets with HIV virions in particular. More sensitive in situ detection methods of HIV infection, as well as assays that can distinguish de novo virus production from endocytically engulfed virions, as recently reported65, will be helpful in determining the exact contribution of stromal DCs to the propagation of HIV.

HIV infection of cervicovaginal CD4+ T cells. CD4+ T cells are dispersed throughout the lamina propria of the human vagina, ectocervix and endocervix, often clustering just beneath the basal membrane66,67. They are also found at variable numbers within the vaginal and ectocervical squamous epithelium66,67. The majority of these cells are memory T cells that express higher levels of CCR5 than those that circulate in the blood25,68,69,70. One day after HIV-1 inoculation of vaginal, ectocervical and endocervical tissue cultures, infected CD4+ T cells were shown to be confined to the mucosal stroma14,16,23,42. This result was surprising, given the presence of CCR5+ CD4+ T cells within the squamous epithelium. However, by analysing the fate of fluorescently tagged virions as early as 2 hours after viral exposure, we observed that R5-tropic HIV-1 efficiently bound to intraepithelial vaginal CD4+ T cells, and this was followed by fusion and productive infection25. Therefore, infected T cells must rapidly leave the epithelium, and those found in the stroma may be the same or the early progeny of intraepithelial T cells.

Findings in the human explant studies show that HIV-1 very effectively targets CD4+ T cells in the genital mucosa for productive infection14,25,42, and that the initial infection of intraepithelial CD4+ T cells is probably independent of LCs25. The central role for genital CD4+ T cells in early infection and propagation is also evident from SIV challenge experiments in macaques62,71,72. Interestingly, not only does SIV productively infect activated T cells, characterized by HLA-DR and Ki67 expression, it also infects T cells that are in the resting state (HLA-DR− and Ki67− T cells)71. Consistent with this finding, we observed binding of HIV-1 to both HLA-DR+ and HLA-DR− intraepithelial T cells in our human vaginal explant model (M.J.M., P. Sakchalathorn, L. Ballweber and F.H., unpublished observations). In addition, the contribution of resting CD4+ T cells to viral production is substantial during the very earliest stages of infection73. The fact that vaginal CD4+ T cells are rapidly depleted following intravenous SIV inoculation of macaques5,72, similar to the depletion of CD4+ T cells in the gut during acute SIV infection74, further illustrates their high susceptibility to infection in vivo.

Other leukocyte HIV targets in the female genital tract. Macrophages in the female genital mucosa are also susceptible targets for early HIV-1 infection, as demonstrated in studies using human cervical explant models23,41,43, and in two reports these cells were the major cell type that was infected by R5-tropic HIV-1 (Refs 23, 43). Whether or not resident macrophages in the female genital tract constitutively express CCR5 in situ is not known, but most macrophages do so when harvested from supernatants of vaginal organ cultures60, suggesting that the expression of CCR5 by macrophages may occur during the period of activation and emigration from the mucosa75. By contrast, SIV-infected macrophages in genital tissues were either rare71, or undetectable30,61. Likewise, macrophages in the human intestinal mucosa were reported to lack CCR5 expression and to possess low permissibility to HIV-1 infection76. These discrepancies illustrate a potential limitation of organ cultures. If explantation activates stromal macrophages and as a consequence increases surface CCR5 expression, this would lead to an overestimation of their susceptibility to infection in vivo. Of note, in addition to allowing virion fusion through their expression of chemokine receptors, monocyte-derived macrophages can also trap intact virions through syndecans77, or even without specific envelope-receptor interactions through a process known as macropinocytosis78. Once captured, HIV-1 can be stored for several days and then transmitted to T cells in trans79,80. If genital macrophages similarly store infectious virions, their role in viral propagation once HIV-1 invades the stroma could be significant.

Other leukocyte populations also interact with HIV. For example, productive infection of natural killer (NK) cells has been reported81, as well as the transmission of HIV through DC-SIGN expressed by B cells to T cells in trans82. The significance of B cells for HIV invasion in the genital mucosa remains unknown. Last, monocyte precursor cells enter the mouse dermis in large numbers following intracutaneous infection with Leishmania major and differentiate into stromal DCs83. This finding raises the possibility that the influx of inflammatory cells into the genital tract following HIV-1 exposure may create new potential target cells that normally do not reside in the mucosa, and further heighten the initial local infection.

The role of DCs in enhancement of infection. A growing body of published literature suggests that HIV exploits DCs to enhance its infectivity of T cells84. First reported in 1992, blood-derived DCs that were exposed to HIV-1 invoked vigorous cytopathic infection of co-cultured CD4+ T cells, even though infection of the DCs could not be detected85. The potential relevance of these findings for the transmission of HIV at mucosal sites was subsequently highlighted by reports of increased HIV-1 replication in DC–T-cell conjugates derived from human skin86 and cervicovaginal mucosa60. The enhancement of HIV transmission by DCs probably occurred by facilitating T-cell activation71,87, as well as de novo T-cell infection.

Four mechanisms for how DCs can augment de novo infection of T cells have been proposed (Fig. 4). In classic HIV trans infection46, although the DCs are not productively infected they can trap and preserve the virus and subsequently transfer it to T cells across an 'infectious synapse', which is a zone of DC–T-cell contact where HIV itself and the HIV host-cell receptors are concentrated88,89. Alternatively, trans infection may occur by HIV association with DC-derived exosomes, which, intriguingly, appear to markedly increase the infectivity of virions that are coupled to them90. The third mechanism involves the transmission of new viral progeny from productively infected DCs across the infectious synapse to T cells48,51,52,53. In this case, the contact zone has also been termed a 'virological synapse', to indicate that the donor cell, in analogy to cell-associated HIV transmission between CD4+ T cells91, is productively infected92. Furthermore, efficient retroviral transfer between cells has recently been described, in which retroviruses, including HIV-1, 'surf' along the outer surface of filopodia or cytonemes that extend from an uninfected cell and interact through their tips with an infected cell93. These narrow filopodial contact zones may be special cases of virological synapses94, or may be analogous to nanotubules that are formed between immune cells95. In T cells, migration of HIV-1 also occurs within nanotubules129. Nanotubules have been shown to functionally connect DCs with other cells95, but their significance for viral transfer from DCs remains to be determined.

Clear evidence for any of these described modes of viral transmission from DCs to CD4+ T cells in the genital mucosa is still lacking. We have shown that HIV concentrates along the cell–cell junction between emigrant LC–T-cell conjugates from human vaginal epithelium, supporting the formation of an infectious synapse25. No consensus has been reached over whether in trans infection occurs primarily from surface bound virions46,96, internalized virions97 or both56. At any rate, once HIV makes its way into the genital DC–T-cell conjugate, a profound productive infection ensues60. Of note, in these conjugates viral budding was not only observed from the surface of T cells but also from the surface of the DCs. Visualization of budding by electron microscopy would suggest that the cells are heavily infected with the virus. So, in genital DC–T-cell conjugates not only T cells but also DCs, which alone are generally weak producers84, produce large amounts of viral progeny. This is consistent with findings obtained in co-cultures of monocyte-derived DCs and T cells98. Therefore, DC–T-cell crosstalk in the genital tract appears to drive productive HIV infection in both cell types. Moreover, transmission from DCs to T cells may provide a means for HIV to avoid antibody-mediated neutralization99,100,101. Taken together, unravelling the precise mechanisms by which the interaction between genital DCs and CD4+ T cells enhance HIV transmission will be important in developing effective strategies to counteract this process.

HIV invasion in the male genital tract

Of the nearly 15 million men that are currently infected with HIV-1, an estimated 70–75% acquired it through vaginal intercourse (Table 1), making the male genital tract the second leading site of HIV invasion. HIV-1 target cells are abundant in the foreskin, and include CD1a+ LCs and CD4+ T cells in the squamous lining, as well as T cells, macrophages and DCs in the underlying stroma45,102,103,104. Variable fractions of these cells express CD4, CCR5 and CXCR4 (Refs 45, 102, 103). As in the vagina, LCs do not express DC-SIGN, whereas at least some of the stromal DCs do102. Therefore, similar mechanisms to the ones that have been described for viral invasion of the female genital tract are likely to occur in the male genital tract.

The highly protective effect of circumcision105,106, which has been reviewed comprehensively elsewhere107, suggests that the penile foreskin is particularly vulnerable to HIV infection. The foreskin is lined by stratified squamous epithelium, and the external surface is more heavily keratinized than the internal surface102,103. Consequently, the inner foreskin may be more susceptible to infection, a view that was supported by investigations of penile autopsy tissues102, and corroborated in an in vitro foreskin explant model that showed infectious foci, predominantly containing LCs and CD4+ T cells, at the base of the epithelial-cell layer, exclusively in the inner foreskin103. Alternatively, circumcision may reduce the risk for genital tears, abrasions and ulcer disease, resulting in decreased HIV infection risk107,108.

Although circumcision appears to provide a protective effect against HIV infection, in circumcised males, there must be HIV entry sites other than the foreskin. The glans penis has a heavily keratinized squamous epithelium similar to the outer foreskin, and effective viral penetration seems relatively improbable here. By contrast, the penile urethra is a more likely candidate as it is lined by narrowly stratified, non-keratinized columnar epithelial cells and contains high numbers of CD4+ and CD8+ T cells, and macrophages, within the epithelium and the lamina propria109. Interestingly, DCs are not observed in the urethral mucosa45,109. The presence of CCR5 and CXCR4 mRNA in urethral swabs indicates that these HIV-1 co-receptors may be expressed by urethral cells110, particularly the predominant CD4+CD45RO+ memory T cells109. Of note, intraurethral infusion of SIV resulted in infection of all six inoculated male macaques111. In men infected with HIV-1, antibiotic treatment of urethral Neisseria gonorrhoeae infection reduces HIV-1 shedding in semen but not the viral load in blood112. This finding, as well as HIV-1 shedding in the ejaculate of vasectomized HIV-positive men113 and in the pre-ejaculatory fluid114,115 suggest a distal viral source, pointing to the urethra as a site harbouring significant numbers of cells susceptible to HIV-1 infection. Taken together, these data suggest that CD4+ T cells and macrophages in the male urethra provide a suitable entry portal for HIV, although the specific events of urethral invasion are still unknown.

Conclusions

Although studies that focus on mucosal HIV infection continue to be painstaking, many investigators have overcome some of the technical difficulties that have previously precluded active research in this area. The recent investigations summarized here provide invaluable insights into the distinct cellular and molecular interactions of mucosal HIV infection. Clearly, the challenge emerging from these findings is to counteract the rapid acceleration of infection in local reservoirs of the lower genital and gastrointestinal tracts. Two key questions to address in future studies lie in determining whether DC–T-cell interactions that markedly amplify HIV-1 production — which are consistently observed in vitro — are relevant in the mucosal epithelium in vivo, and whether common mechanisms of HIV-1 entry apply to both the lower genital and gastrointestinal tract. This information can guide the development of innovative strategies to protect susceptible target cells from HIV-1 infection in both women and men, such as with barrier protection methods, topical microbicides and mucosal immunization.

Although there is an increasing number of large-scale HIV prevention clinical trials that have recently reported a lack of efficacy, one favourable approach has been male circumcision. The opportunity to gain a more thorough understanding of how HIV-1 invades its target cells in the human foreskin should be exploited. The recent emphasis of CD4+ T-cell depletion by HIV-1 in the gut and vagina during acute infection has sparked a renewed interest in abating infection at the sites where massive replication occurs116. Although important in counteracting HIV disease, CD4+ T-cell depletion appears to be a secondary event that commonly occurs through various routes of transmission. Therefore, the best opportunity to prevent HIV disease clearly lies at the sites of mucosal entry, and investigations to directly counterattack HIV infection must continue to focus on these portals.

References

  1. Kell, P. D., Barton, S. E., Edmonds, D. K. & Boag, F. C. HIV infection in a patient with Meyer-Rokitansky-Kuster-Hauser syndrome. J. R. Soc. Med. 85, 706–707 (1992).
    CAS PubMed PubMed Central Google Scholar
  2. Miller, C. J., Alexander, N. J., Vogel, P., Anderson, J. & Marx, P. A. Mechanism of genital transmission of SIV: a hypothesis based on transmission studies and the location of SIV in the genital tract of chronically infected female rhesus macaques. J. Med. Primatol. 21, 64–68 (1992).
    CAS PubMed Google Scholar
  3. Padian, N. S. et al. Diaphragm and lubricant gel for prevention of HIV acquisition in southern African women: a randomised controlled trial. Lancet 370, 251–261 (2007).
    Article PubMed PubMed Central Google Scholar
  4. Howell, A. L. et al. Human immunodeficiency virus type 1 infection of cells and tissues from the upper and lower human female reproductive tract. J. Virol. 71, 3498–3506 (1997).
    CAS PubMed PubMed Central Google Scholar
  5. Joag, S. V. et al. Animal model of mucosally transmitted human immunodeficiency virus type 1 disease: intravaginal and oral deposition of simian/human immunodeficiency virus in macaques results in systemic infection, elimination of CD4+ T cells, and AIDS. J. Virol. 71, 4016–4023 (1997).
    CAS PubMed PubMed Central Google Scholar
  6. Phillips, D. M., Tan, X., Perotti, M. E. & Zacharopoulos, V. R. Mechanism of monocyte-macrophage-mediated transmission of HIV. AIDS Res. Hum. Retroviruses 14, S67–S70 (1998).
    PubMed Google Scholar
  7. Alfsen, A., Yu, H., Magerus-Chatinet, A., Schmitt, A. & Bomsel, M. HIV-1-infected blood mononuclear cells form an integrin- and agrin-dependent viral synapse to induce efficient HIV-1 transcytosis across epithelial cell monolayer. Mol. Biol. Cell 16, 4267–4279 (2005).
    Article CAS PubMed PubMed Central Google Scholar
  8. Sourisseau, M., Sol-Foulon, N., Porrot, F., Blanchet, F. & Schwartz, O. Inefficient human immunodeficiency virus replication in mobile lymphocytes. J. Virol. 81, 1000–1012 (2007).
    Article CAS PubMed Google Scholar
  9. Muratori, C. et al. Macrophages transmit human immunodeficiency virus type 1 products to CD4-negative cells: involvement of matrix metalloproteinase 9. J. Virol. 81, 9078–9087 (2007).
    Article CAS PubMed PubMed Central Google Scholar
  10. Van Herrewege, Y. et al. A dual chamber model of female cervical mucosa for the study of HIV transmission and for the evaluation of candidate HIV microbicides. Antiviral Res. 74, 111–124 (2007).
    Article CAS PubMed Google Scholar
  11. Kaizu, M. et al. Repeated intravaginal inoculation with cell-associated simian immunodeficiency virus results in persistent infection of nonhuman primates. J. Infect. Dis. 194, 912–916 (2006).
    Article CAS PubMed Google Scholar
  12. Khanna, K. V. et al. Vaginal transmission of cell-associated HIV-1 in the mouse is blocked by a topical, membrane-modifying agent. J. Clin. Invest. 109, 205–211 (2002).
    Article CAS PubMed PubMed Central Google Scholar
  13. Zhu, T. et al. Genetic characterization of human immunodeficiency virus type 1 in blood and genital secretions: evidence for viral compartmentalization and selection during sexual transmission. J. Virol. 70, 3098–3107 (1996).
    CAS PubMed PubMed Central Google Scholar
  14. Gupta, P. et al. Memory CD4+ T cells are the earliest detectable human immunodeficiency virus type 1 (HIV-1)-infected cells in the female genital mucosal tissue during HIV-1 transmission in an organ culture system. J. Virol. 76, 9868–9876 (2002).
    Article CAS PubMed PubMed Central Google Scholar
  15. Zussman, A., Lara, L., Lara, H. H., Bentwich, Z. & Borkow, G. Blocking of cell-free and cell-associated HIV-1 transmission through human cervix organ culture with UC781. AIDS 17, 653–661 (2003).
    Article CAS PubMed Google Scholar
  16. Maher, D., Wu, X., Schacker, T., Horbul, J. & Southern, P. HIV binding, penetration, and primary infection in human cervicovaginal tissue. Proc. Natl Acad. Sci. USA 102, 11504–11509 (2005).
    Article CAS PubMed PubMed Central Google Scholar
  17. Miller, C. J. et al. Propagation and dissemination of infection after vaginal transmission of simian immunodeficiency virus. J. Virol. 79, 9217–9227 (2005).
    Article CAS PubMed PubMed Central Google Scholar
  18. Dezzutti, C. S. et al. Cervical and prostate primary epithelial cells are not productively infected but sequester human immunodeficiency virus type 1. J. Infect. Dis. 183, 1204–1213 (2001).
    Article CAS PubMed Google Scholar
  19. Wu, Z., Chen, Z. & Phillips, D. M. Human genital epithelial cells capture cell-free human immunodeficiency virus type 1 and transmit the virus to CD4+ cells: implications for mechanisms of sexual transmission. J. Infect. Dis. 188, 1473–1482 (2003).
    Article PubMed Google Scholar
  20. Berlier, W. et al. Selective sequestration of X4 isolates by human genital epithelial cells: Implication for virus tropism selection process during sexual transmission of HIV. J. Med. Virol. 77, 465–474 (2005).
    Article CAS PubMed Google Scholar
  21. Bomsel, M. Transcytosis of infectious human immunodeficiency virus across a tight human epithelial cell line barrier. Nature Med. 3, 42–47 (1997). This study introduced the concept of HIV-1 transcytosis, a process by which intact virions are transported through the interior of epithelial cells, thereby retaining their infectivity.
    Article CAS PubMed Google Scholar
  22. Bobardt, M. D. et al. Cell-free human immunodeficiency virus type 1 transcytosis through primary genital epithelial cells. J. Virol. 81, 395–405 (2007).
    Article CAS PubMed Google Scholar
  23. Greenhead, P. et al. Parameters of human immunodeficiency virus infection of human cervical tissue and inhibition by vaginal virucides. J. Virol. 74, 5577–5586 (2000).
    Article CAS PubMed PubMed Central Google Scholar
  24. Ibata, B., Parr, E. L., King, N. J. & Parr, M. B. Migration of foreign lymphocytes from the mouse vagina into the cervicovaginal mucosa and to the iliac lymph nodes. Biol. Reprod. 56, 537–543 (1997).
    Article CAS PubMed Google Scholar
  25. Hladik, F. et al. Initial events in establishing vaginal entry and infection by human immunodeficiency virus type-1. Immunity 26, 257–270 (2007). This investigation found that HIV-1 rapidly penetrates intraepithelial vaginal CD1a+ Langerhans cells and CD4+ T cells, and that viral fusion predominates in T cells and viral endocytosis in LCs.
    Article CAS PubMed PubMed Central Google Scholar
  26. Munch, J. et al. Semen-derived amyloid fibrils drastically enhance HIV infection. Cell 131, 1059–1071 (2007). The authors discovered that amyloidogenic fragments of prostatic acidic phosphatase in semen can serve as strong enhancing factors for HIV infection.
    Article CAS PubMed Google Scholar
  27. Furuta, Y. et al. Infection of vaginal and colonic epithelial cells by the human immunodeficiency virus type 1 is neutralized by antibodies raised against conserved epitopes in the envelope glycoprotein gp120. Proc. Natl Acad. Sci. USA 91, 12559–12563 (1994).
    Article CAS PubMed PubMed Central Google Scholar
  28. Yeaman, G. R. et al. Chemokine receptor expression in the human ectocervix: implications for infection by the human immunodeficiency virus-type I. Immunology 113, 524–533 (2004).
    Article CAS PubMed PubMed Central Google Scholar
  29. Stoddard, E. et al. gp340 expressed on human genital epithelia binds HIV-1 envelope protein and facilitates viral transmission. J. Immunol. 179, 3126–3132 (2007).
    Article CAS PubMed Google Scholar
  30. Hu, J., Gardner, M. B. & Miller, C. J. Simian immunodeficiency virus rapidly penetrates the cervicovaginal mucosa after intravaginal inoculation and infects intraepithelial dendritic cells. J. Virol. 74, 6087–6095 (2000).
    Article CAS PubMed PubMed Central Google Scholar
  31. Nishibu, A. et al. Behavioral responses of epidermal Langerhans cells in situ to local pathological stimuli. J. Invest. Dermatol. 126, 787–796 (2006).
    Article CAS PubMed Google Scholar
  32. Miller, C. J., McChesney, M. & Moore, P. F. Langerhans cells, macrophages and lymphocyte subsets in the cervix and vagina of rhesus macaques. Lab. Invest. 67, 628–634 (1992).
    CAS PubMed Google Scholar
  33. Rescigno, M. et al. Dendritic cells express tight junction proteins and penetrate gut epithelial monolayers to sample bacteria. Nature Immunol. 2, 361–367 (2001).
    Article CAS Google Scholar
  34. Niess, J. H. et al. CX3CR1-mediated dendritic cell access to the intestinal lumen and bacterial clearance. Science 307, 254–258 (2005).
    Article CAS PubMed Google Scholar
  35. Norvell, M. K., Benrubi, G. I. & Thompson, R. J. Investigation of microtrauma after sexual intercourse. J. Reprod. Med. 29, 269–271 (1984).
    CAS PubMed Google Scholar
  36. Weiler, A. M. et al. Genital ulcers facilitate rapid viral entry and dissemination following intravaginal inoculation with cell-associated SIVmac239. J. Virol. 82, 4154–4158 (2008).
    Article CAS PubMed PubMed Central Google Scholar
  37. Tschachler, E. et al. Epidermal Langerhans cells—a target for HTLV-III/LAV infection. J. Invest. Dermatol. 88, 233–237 (1987).
    Article CAS PubMed Google Scholar
  38. Zaitseva, M. et al. Expression and function of CCR5 and CXCR4 on human Langerhans cells and macrophages: implications for HIV primary infection. Nature Med. 3, 1369–1375 (1997).
    Article CAS PubMed Google Scholar
  39. Dittmar, M. T. et al. Langerhans cell tropism of human immunodeficiency virus type 1 subtype A through F isolates derived from different transmission groups. J. Virol. 71, 8008–8013 (1997).
    CAS PubMed PubMed Central Google Scholar
  40. Kawamura, T. et al. Candidate microbicides block HIV-1 infection of human immature Langerhans cells within epithelial tissue explants. J. Exp. Med. 192, 1491–1500 (2000).
    Article CAS PubMed PubMed Central Google Scholar
  41. Collins, K. B., Patterson, B. K., Naus, G. J., Landers, D. V. & Gupta, P. Development of an in vitro organ culture model to study transmission of HIV-1 in the female genital tract. Nature Med. 6, 475–479 (2000).
    Article CAS PubMed Google Scholar
  42. Hu, Q. et al. Blockade of attachment and fusion receptors inhibits HIV-1 infection of human cervical tissue. J. Exp. Med. 199, 1065–1075 (2004). This investigation clearly demonstrates that DCs migrating from HIV-1-exposed cervical tissue can efficiently transmit the virus. Inhibition of this pathway can occur only by simultaneous blockade of CD4 and mannose-binding C-type lectin receptors.
    Article CAS PubMed PubMed Central Google Scholar
  43. Cummins, J. E., Jr et al. Preclinical testing of candidate topical microbicides for anti-human immunodeficiency virus type 1 activity and tissue toxicity in a human cervical explant culture. Antimicrob. Agents Chemother. 51, 1770–1779 (2007).
    Article CAS PubMed PubMed Central Google Scholar
  44. Turville, S. G. et al. Diversity of receptors binding HIV on dendritic cell subsets. Nature Immunol. 3, 975–983 (2002).
    Article CAS Google Scholar
  45. Hussain, L. A. & Lehner, T. Comparative investigation of Langerhans' cells and potential receptors for HIV in oral, genitourinary and rectal epithelia. Immunology 85, 475–484 (1995).
    CAS PubMed PubMed Central Google Scholar
  46. Geijtenbeek, T. B. et al. DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell 100, 587–597 (2000). In this report, DC-SIGN is identified as an HIV-1 receptor expressed by DCs that promotes efficient infection of CD4+ T cells in trans.
    Article CAS PubMed Google Scholar
  47. Jameson, B. et al. Expression of DC-SIGN by dendritic cells of intestinal and genital mucosae in humans and rhesus macaques. J. Virol. 76, 1866–1875 (2002).
    Article CAS PubMed PubMed Central Google Scholar
  48. Kawamura, T. et al. Significant virus replication in Langerhans cells following application of HIV to abraded skin: relevance to occupational transmission of HIV. J. Immunol. 180, 3297–3304 (2008).
    Article CAS PubMed Google Scholar
  49. Pope, M., Gezelter, S., Gallo, N., Hoffman, L. & Steinman, R. M. Low levels of HIV-1 infection in cutaneous dendritic cells promote extensive viral replication upon binding to memory CD4+ T cells. J. Exp. Med. 182, 2045–2056 (1995).
    Article CAS PubMed Google Scholar
  50. Cameron, P. U. et al. Preferential infection of dendritic cells during human immunodeficiency virus type 1 infection of blood leukocytes. J. Virol. 81, 2297–2306 (2007).
    Article CAS PubMed Google Scholar
  51. Nobile, C. et al. Covert human immunodeficiency virus replication in dendritic cells and in DC-SIGN-expressing cells promotes long-term transmission to lymphocytes. J. Virol. 79, 5386–5399 (2005).
    Article CAS PubMed PubMed Central Google Scholar
  52. Burleigh, L. et al. Infection of dendritic cells (DCs), not DC-SIGN-mediated internalization of human immunodeficiency virus, is required for long-term transfer of virus to T cells. J. Virol. 80, 2949–2957 (2006).
    Article CAS PubMed PubMed Central Google Scholar
  53. Turville, S. G. et al. Immunodeficiency virus uptake, turnover, and 2-phase transfer in human dendritic cells. Blood 103, 2170–2179 (2004).
    Article CAS PubMed Google Scholar
  54. Gummuluru, S., Rogel, M., Stamatatos, L. & Emerman, M. Binding of human immunodeficiency virus type 1 to immature dendritic cells can occur independently of DC-SIGN and mannose binding C-type lectin receptors via a cholesterol-dependent pathway. J. Virol. 77, 12865–12874 (2003).
    Article CAS PubMed PubMed Central Google Scholar
  55. Boggiano, C., Manel, N. & Littman, D. R. Dendritic cell-mediated _trans_-enhancement of human immunodeficiency virus type 1 infectivity is independent of DC-SIGN. J. Virol. 81, 2519–2523 (2007).
    Article CAS PubMed Google Scholar
  56. Wang, J. H., Janas, A. M., Olson, W. J. & Wu, L. Functionally distinct transmission of human immunodeficiency virus type 1 mediated by immature and mature dendritic cells. J. Virol. 81, 8933–8943 (2007).
    Article CAS PubMed PubMed Central Google Scholar
  57. de Witte, L. et al. Langerin is a natural barrier to HIV-1 transmission by Langerhans cells. Nature Med. 13, 367–371 (2007). This study showed that HIV-1 captured by the C-type lectin receptor langerin was internalized into Birbeck granules and degraded, therefore preventing HIV-1 transmission by skin LCs.
    Article CAS PubMed Google Scholar
  58. Savina, A. et al. NOX2 controls phagosomal pH to regulate antigen processing during crosspresentation by dendritic cells. Cell 126, 205–218 (2006).
    Article CAS PubMed Google Scholar
  59. Prakash, M., Kapembwa, M. S., Gotch, F. & Patterson, S. Chemokine receptor expression on mucosal dendritic cells from the endocervix of healthy women. J. Infect. Dis. 190, 246–250 (2004).
    Article CAS PubMed Google Scholar
  60. Hladik, F. et al. Dendritic cell-T-cell interactions support coreceptor-independent human immunodeficiency virus type 1 transmission in the human genital tract. J. Virol. 73, 5833–5842 (1999). This is the first reported observation that DCs isolated from the vaginal mucosa internalize HIV-1 into cytoplasmic endosomes and produce new virions that bud from the cell membrane.
    CAS PubMed PubMed Central Google Scholar
  61. Spira, A. I. et al. Cellular targets of infection and route of viral dissemination after an intravaginal inoculation of simian immunodeficiency virus into rhesus macaques. J. Exp. Med. 183, 215–225 (1996). In this study, intravaginal inoculation of macaques with SIV led to the infection of stromal DCs and cells in the draining lymph nodes within two days.
    Article CAS PubMed Google Scholar
  62. Hu, J., Pope, M., Brown, C., O'Doherty, U. & Miller, C. J. Immunophenotypic characterization of simian immunodeficiency virus- infected dendritic cells in cervix, vagina, and draining lymph nodes of rhesus monkeys. Lab. Invest. 78, 435–451 (1998).
    CAS PubMed Google Scholar
  63. Bhoopat, L. et al. In vivo identification of Langerhans and related dendritic cells infected with HIV-1 subtype E in vaginal mucosa of asymptomatic patients. Mod. Pathol. 14, 1263–1269 (2001).
    Article CAS PubMed Google Scholar
  64. Nestle, F. O. & Nickoloff, B. J. Deepening our understanding of immune sentinels in the skin. J. Clin. Invest. 117, 2382–2385 (2007).
    Article CAS PubMed PubMed Central Google Scholar
  65. Turville, S. G., Aravantinou, M., Stossel, H., Romani, N. & Robbiani, M. Resolution of de novo HIV production and trafficking in immature dendritic cells. Nature Methods 5, 75–85 (2008).
    Article CAS PubMed Google Scholar
  66. Johansson, E. L., Rudin, A., Wassen, L. & Holmgren, J. Distribution of lymphocytes and adhesion molecules in human cervix and vagina. Immunology 96, 272–277 (1999).
    Article CAS PubMed PubMed Central Google Scholar
  67. Edwards, J. N. & Morris, H. B. Langerhans' cells and lymphocyte subsets in the female genital tract. Br. J. Obstet. Gynaecol. 92, 974–982 (1985).
    Article CAS PubMed Google Scholar
  68. Hladik, F., Lentz, G., Delpit, E., McElroy, A. & McElrath, M. J. Coexpression of CCR5 and IL-2 in human genital but not blood T cells: implications for the ontogeny of the CCR5+ Th1 phenotype. J. Immunol. 163, 2306–2313 (1999).
    CAS PubMed Google Scholar
  69. Zhang, L. et al. In vivo distribution of the human immunodeficiency virus/simian immunodeficiency virus coreceptors: CXCR4, CCR3, and CCR5. J. Virol. 72, 5035–5045 (1998).
    CAS PubMed PubMed Central Google Scholar
  70. Prakash, M., Kapembwa, M. S., Gotch, F. & Patterson, S. Higher levels of activation markers and chemokine receptors on T lymphocytes in the cervix than peripheral blood of normal healthy women. J. Reprod. Immunol. 52, 101–111 (2001).
    Article CAS PubMed Google Scholar
  71. Zhang, Z. et al. Sexual transmission and propagation of SIV and HIV in resting and activated CD4+ T cells. Science 286, 1353–1357 (1999). The authors identified CD4+ T cells in the macaque genital mucosa as the predominant targets for SIV infection, and they noted that both activated and resting T cells propagate virus.
    Article CAS PubMed Google Scholar
  72. Veazey, R. S., Marx, P. A. & Lackner, A. A. Vaginal CD4+ T cells express high levels of CCR5 and are rapidly depleted in simian immunodeficiency virus infection. J. Infect. Dis. 187, 769–776 (2003).
    Article CAS PubMed Google Scholar
  73. Zhang, Z. Q. et al. Roles of substrate availability and infection of resting and activated CD4+ T cells in transmission and acute simian immunodeficiency virus infection. Proc. Natl Acad. Sci. USA 101, 5640–5645 (2004).
    Article CAS PubMed PubMed Central Google Scholar
  74. Picker, L. J. & Watkins, D. I. HIV pathogenesis: the first cut is the deepest. Nature Immunol. 6, 430–432 (2005).
    Article CAS Google Scholar
  75. Tuttle, D. L., Harrison, J. K., Anders, C., Sleasman, J. W. & Goodenow, M. M. Expression of CCR5 increases during monocyte differentiation and directly mediates macrophage susceptibility to infection by human immunodeficiency virus type 1. J. Virol. 72, 4962–4969 (1998).
    CAS PubMed PubMed Central Google Scholar
  76. Meng, G. et al. Lamina propria lymphocytes, not macrophages, express CCR5 and CXCR4 and are the likely target cell for human immunodeficiency virus type 1 in the intestinal mucosa. J. Infect. Dis. 182, 785–791 (2000).
    Article CAS PubMed Google Scholar
  77. Saphire, A. C., Bobardt, M. D., Zhang, Z., David, G. & Gallay, P. A. Syndecans serve as attachment receptors for human immunodeficiency virus type 1 on macrophages. J. Virol. 75, 9187–9200 (2001).
    Article CAS PubMed PubMed Central Google Scholar
  78. Marechal, V. et al. Human immunodeficiency virus type 1 entry into macrophages mediated by macropinocytosis. J. Virol. 75, 11166–11177 (2001).
    Article CAS PubMed PubMed Central Google Scholar
  79. Sharova, N., Swingler, C., Sharkey, M. & Stevenson, M. Macrophages archive HIV-1 virions for dissemination in trans. EMBO J. 24, 2481–2489 (2005).
    Article CAS PubMed PubMed Central Google Scholar
  80. Groot, F., Welsch, S. & Sattentau, Q. J. Efficient HIV-1 transmission from macrophages to T cells across transient virological synapses. Blood, 22 February 2008 (doi:10.1182/blood-2007-12-130070).
    Article CAS PubMed Google Scholar
  81. Harada, H., Goto, Y., Ohno, T., Suzu, S. & Okada, S. Proliferative activation up-regulates expression of CD4 and HIV-1 co-receptors on NK cells and induces their infection with HIV-1. Eur. J. Immunol. 37, 2148–2155 (2007).
    Article CAS PubMed Google Scholar
  82. Rappocciolo, G. et al. DC-SIGN on B lymphocytes is required for transmission of HIV-1 to T lymphocytes. PLoS Pathog. 2, e70 (2006).
    Article PubMed PubMed Central Google Scholar
  83. Leon, B., Lopez-Bravo, M. & Ardavin, C. Monocyte-derived dendritic cells formed at the infection site control the induction of protective T helper 1 responses against Leishmania. Immunity 26, 519–531 (2007).
    Article CAS PubMed Google Scholar
  84. Wu, L. & KewalRamani, V. N. Dendritic-cell interactions with HIV: infection and viral dissemination. Nature Rev. Immunol. 6, 859–868 (2006).
    Article CAS Google Scholar
  85. Cameron, P. U. et al. Dendritic cells exposed to human immunodeficiency virus type-1 transmit a vigorous cytopathic infection to CD4+ T cells. Science 257, 383–387 (1992). This is the first study demonstrating HIV-1 transmission from DCs to CD4+ T cells.
    Article CAS PubMed Google Scholar
  86. Pope, M. et al. Conjugates of dendritic cells and memory T lymphocytes from skin facilitate productive infection with HIV-1. Cell 78, 389–398 (1994). This investigation showed that DCs and T cells derived from human epithelium form stable conjugates which can enhance HIV replication.
    Article CAS PubMed Google Scholar
  87. Geijtenbeek, T. B. et al. Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell 100, 575–585 (2000).
    Article CAS PubMed Google Scholar
  88. McDonald, D. et al. Recruitment of HIV and its receptors to dendritic cell-T cell junctions. Science 300, 1295–1297 (2003). The investigators introduce the concept of an infectious synapse between DCs and T cells to which HIV is recruited on the DC side and CD4 and CCR5 on the T-cell side, thus promoting efficient in trans infection.
    Article CAS PubMed Google Scholar
  89. Arrighi, J. F. et al. DC-SIGN-mediated infectious synapse formation enhances X4 HIV-1 transmission from dendritic cells to T cells. J. Exp. Med. 200, 1279–1288 (2004).
    Article CAS PubMed PubMed Central Google Scholar
  90. Wiley, R. D. & Gummuluru, S. Immature dendritic cell-derived exosomes can mediate HIV-1 trans infection. Proc. Natl Acad. Sci. USA 103, 738–743 (2006).
    Article CAS PubMed PubMed Central Google Scholar
  91. Jolly, C. & Sattentau, Q. J. Human immunodeficiency virus type 1 assembly, budding, and cell-cell spread in T cells take place in tetraspanin-enriched plasma membrane domains. J. Virol. 81, 7873–7884 (2007).
    Article CAS PubMed PubMed Central Google Scholar
  92. Jolly, C. & Sattentau, Q. J. Retroviral spread by induction of virological synapses. Traffic 5, 643–650 (2004).
    Article CAS PubMed Google Scholar
  93. Sherer, N. M. et al. Retroviruses can establish filopodial bridges for efficient cell-to-cell transmission. Nature Cell Biol. 9, 310–315 (2007). Retroviruses are transmitted between cells not only across large-surface interfaces (infectious synapses) but also through movement along the outer surface of thin filopodial bridges that are extended from the non-infected to the infected cells.
    Article CAS PubMed Google Scholar
  94. Hope, T. J. Bridging efficient viral infection. Nature Cell Biol. 9, 243–244 (2007).
    Article CAS PubMed Google Scholar
  95. Watkins, S. C. & Salter, R. D. Functional connectivity between immune cells mediated by tunneling nanotubules. Immunity 23, 309–318 (2005).
    Article CAS PubMed Google Scholar
  96. Cavrois, M., Neidleman, J., Kreisberg, J. F. & Greene, W. C. In vitro derived dendritic cells _trans_-infect CD4 T cells primarily with surface-bound HIV-1 virions. PLoS Pathog. 3, e4 (2007).
    Article CAS PubMed PubMed Central Google Scholar
  97. Kwon, D. S., Gregorio, G., Bitton, N., Hendrickson, W. A. & Littman, D. R. DC-SIGN-mediated internalization of HIV is required for trans-enhancement of T cell infection. Immunity 16, 135–144 (2002).
    Article CAS PubMed Google Scholar
  98. Granelli-Piperno, A., Finkel, V., Delgado, E. & Steinman, R. M. Virus replication begins in dendritic cells during the transmission of HIV-1 from mature dendritic cells to T cells. Curr. Biol. 9, 21–29 (1999).
    Article CAS PubMed Google Scholar
  99. van Montfort, T., Nabatov, A. A., Geijtenbeek, T. B., Pollakis, G. & Paxton, W. A. Efficient capture of antibody neutralized HIV-1 by cells expressing DC-SIGN and transfer to CD4+ T lymphocytes. J. Immunol. 178, 3177–3185 (2007).
    Article CAS PubMed Google Scholar
  100. Ganesh, L. et al. Infection of specific dendritic cells by CCR5-tropic human immunodeficiency virus type 1 promotes cell-mediated transmission of virus resistant to broadly neutralizing antibodies. J. Virol. 78, 11980–11987 (2004).
    Article CAS PubMed PubMed Central Google Scholar
  101. Chen, P., Hubner, W., Spinelli, M. A. & Chen, B. K. Predominant mode of HIV transfer between T cells is mediated by sustained Env-dependent neutralization-resistant virological synapses. J. Virol. 81, 12582–12595 (2007).
    Article CAS PubMed PubMed Central Google Scholar
  102. McCoombe, S. G. & Short, R. V. Potential HIV-1 target cells in the human penis. AIDS 20, 1491–1495 (2006).
    Article PubMed Google Scholar
  103. Patterson, B. K. et al. Susceptibility to human immunodeficiency virus-1 infection of human foreskin and cervical tissue grown in explant culture. Am. J. Pathol. 161, 867–873 (2002).
    Article PubMed PubMed Central Google Scholar
  104. Donoval, B. A. et al. HIV-1 target cells in foreskins of African men with varying histories of sexually transmitted infections. Am. J. Clin. Pathol. 125, 386–391 (2006).
    Article PubMed Google Scholar
  105. Meier, A. S., Bukusi, E. A., Cohen, C. R. & Holmes, K. K. Independent association of hygiene, socioeconomic status, and circumcision with reduced risk of HIV infection among Kenyan men. J. Acquir. Immune Defic. Syndr. 43, 117–118 (2006).
    Article PubMed Google Scholar
  106. Gray, R. H. et al. Male circumcision for HIV prevention in men in Rakai, Uganda: a randomised trial. Lancet 369, 657–666 (2007).
    Article PubMed Google Scholar
  107. Quinn, T. C. Circumcision and HIV transmission. Curr. Opin. Infect. Dis. 20, 33–38 (2007).
    Article PubMed Google Scholar
  108. Wawer, M. J. et al. Rates of HIV-1 transmission per coital act, by stage of HIV-1 infection, in Rakai, Uganda. J. Infect. Dis. 191, 1403–1409 (2005).
    Article PubMed Google Scholar
  109. Pudney, J. & Anderson, D. J. Immunobiology of the human penile urethra. Am. J. Pathol. 147, 155–165 (1995).
    CAS PubMed PubMed Central Google Scholar
  110. McClure, C. P. et al. HIV coreceptor and chemokine ligand gene expression in the male urethra and female cervix. AIDS 19, 1257–1265 (2005).
    Article CAS PubMed Google Scholar
  111. Miller, C. J. et al. Genital mucosal transmission of simian immunodeficiency virus: animal model for heterosexual transmission of human immunodeficiency virus. J. Virol. 63, 4277–4284 (1989).
    CAS PubMed PubMed Central Google Scholar
  112. Cohen, M. S. et al. Reduction of concentration of HIV-1 in semen after treatment of urethritis: implications for prevention of sexual transmission of HIV-1. AIDSCAP Malawi Research Group. Lancet 349, 1868–1873 (1997).
    Article CAS PubMed Google Scholar
  113. Krieger, J. N. et al. Vasectomy and human immunodeficiency virus type 1 in semen. J. Urol. 159, 820–825; discussion 825–826 (1998).
    Article CAS PubMed Google Scholar
  114. Pudney, J., Oneta, M., Mayer, K., Seage, G., 3rd & Anderson, D. Pre-ejaculatory fluid as potential vector for sexual transmission of HIV-1. Lancet 340, 1470 (1992).
    Article CAS PubMed Google Scholar
  115. Ilaria, G. et al. Detection of HIV-1 DNA sequences in pre-ejaculatory fluid. Lancet 340, 1469 (1992).
    Article CAS PubMed Google Scholar
  116. Brenchley, J. M., Price, D. A. & Douek, D. C. HIV disease: fallout from a mucosal catastrophe? Nature Immunol. 7, 235–239 (2006).
    Article CAS Google Scholar
  117. Wang, X. et al. Massive infection and loss of CD4+ T cells occurs in the intestinal tract of neonatal rhesus macaques in acute SIV infection. Blood 109, 1174–1181 (2007).
    Article CAS PubMed PubMed Central Google Scholar
  118. Mattapallil, J. J. et al. Massive infection and loss of memory CD4+ T cells in multiple tissues during acute SIV infection. Nature 434, 1093–1097 (2005).
    Article CAS PubMed Google Scholar
  119. Mehandru, S. et al. Primary HIV-1 infection is associated with preferential depletion of CD4+ T lymphocytes from effector sites in the gastrointestinal tract. J. Exp. Med. 200, 761–770 (2004).
    Article CAS PubMed PubMed Central Google Scholar
  120. Haase, A. T. Perils at mucosal front lines for HIV and SIV and their hosts. Nature Rev. Immunol. 5, 783–792 (2005).
    Article CAS Google Scholar
  121. HIV/AIDS among men who have sex with men, 2007. CDC HIV/AIDS Fact Sheet. Centers for Diseases Control and Prevention, Atlanta. [online]
  122. The Global HIV/AIDS pandemic. Morb. Mort. Wkly. Rep. 55, 841–844 (2006).
  123. Page-Shafer, K. et al. Risk of HIV infection attributable to oral sex among men who have sex with men and in the population of men who have sex with men. AIDS 16, 2350–2352 (2002).
    Article PubMed Google Scholar
  124. Aceijas, C., Stimson, G. V., Hickman, M. & Rhodes, T. Global overview of injecting drug use and HIV infection among injecting drug users. AIDS 18, 2295–2303 (2004).
    Article PubMed Google Scholar
  125. Lehman, D. A. & Farquhar, C. Biological mechanisms of vertical human immunodeficiency virus (HIV-1) transmission. Rev. Med. Virol. 381–403 (2007).
  126. Galvin, S. R. & Cohen, M. S. The role of sexually transmitted diseases in HIV transmission. Nature Rev. Microbiol. 2, 33–42 (2004).
    Article CAS Google Scholar
  127. Royce, R. A., Sena, A., Cates, W., Jr. & Cohen, M. S. Sexual transmission of HIV. N. Engl. J. Med. 336, 1072–1078 (1997).
    Article CAS PubMed Google Scholar
  128. Halperin, D. T. Heterosexual anal intercourse: prevalence, cultural factors, and HIV infection and other health risks, Part I. Aids Patient Care STDS 13, 717–730 (1999).
    Article CAS PubMed Google Scholar
  129. Sowinski et al. Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission. Nature Cell Biol. 10, 211–219 (2008).
    Article CAS PubMed Google Scholar

Download references

Acknowledgements

We thank L. Ballweber and P. Sakchalathorn for electron microscopy and P. Stegall for editorial assistance. This work was supported by the US National Institutes of Health grants AI51980, HD51455, and the James B. Pendleton Trust.

Author information

Authors and Affiliations

  1. Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research Center, Seattle, 98109, Washington, USA
    Florian Hladik & M. Juliana McElrath
  2. Department of Medicine, University of Washington School of Medicine, Seattle, 98195, Washington, USA
    Florian Hladik & M. Juliana McElrath
  3. Department of Obstetrics and Gynecology, University of Washington School of Medicine, Seattle, 98195, Washington, USA
    Florian Hladik
  4. Department of Global Health and Laboratory Medicine, University of Washington School of Medicine, Seattle, 98195, Washington, USA
    M. Juliana McElrath

Authors

  1. Florian Hladik
    You can also search for this author inPubMed Google Scholar
  2. M. Juliana McElrath
    You can also search for this author inPubMed Google Scholar

Corresponding author

Correspondence toM. Juliana McElrath.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Glossary

Simian–HIV

(SHIV). SHIVs are chimeric viruses that are created by inserting the envelope protein (Env), the transcriptional transactivator (Tat) and the regulator of virion gene expression (Rev) of HIV into the SIVMAC239 clone. Depending on the particular HIV Env protein, these SHIVs have different in vivo characteristics. The SHIV chimeric viruses are best used for testing antibodies specific for HIV in non-human primate models.

Transcytosis

The process of transport of material, including HIV virions, across a cell layer by uptake on one side of the cell into a coated vesicle. The vesicle might then be sorted through the _trans_-Golgi network and transported to the opposite side of the cell, where its contents are released into the extracelluar space.

Langerhans cell

(LC). A type of dendritic cell that is localized in the squamous epithelial layer of the skin and certain mucosae.

Syndecans

Single transmembrane domain proteins that carry three to five heparan sulphate and chondroitin sulphate chains that allow for interaction with various ligands including residues on the HIV-1 gp 120 protein.

Stromal papillae

Superficial areas of the mucosal stroma that interdigitate with the epithelium.

C-type lectin receptors

A large family of receptors that bind glycosylated ligands and have multiple roles, such as in cell adhesion, endocytosis, natural-killer-cell target recognition and dendritic-cell activation.

R5-tropic HIV-1

An HIV strain that uses CC-chemokine receptor 5 (CCR5) as the co-receptor to gain entry to target cells.

Birbeck granules

Membrane-bound rod- or tennis-racket-shaped structures with a central linear density, found in the cytoplasm of Langerhans cells. Their formation is induced by langerin, an endocytic C-type lectin receptor that is specific to Langerhans cells.

Phagosomes

Vacuolar compartments that confine microorganisms after enforced endocytosis or after phagocytosis. Unless counteracted by a microbial survival strategy, the phagosome matures into a hostile environment that is designed to kill and digest microorganisms.

Cross-presentation

The initiation of a CD8+ T-cell response to an antigen that is not present within antigen-presenting cells (APCs). This exogenous antigen must be taken up by APCs and then re-routed to the MHC-class-I pathway of antigen presentation.

Lamina propria

Connective tissue that underlies the epithelium of the mucosa and contains various myeloid and lymphoid cells, including macrophages, dendritic cells, T cells and B cells.

Macropinocytosis

A mechanism of endocytosis in which large droplets of fluid are trapped underneath extensions (ruffles) of the cell surface. Can be exploited by some pathogens as a route for entry into cells.

Exosomes

Small lipid-bilayer vesicles that are released from activated cells. They comprise either plasma membrane or membrane derived from intracellular vesicles.

Filopodia

Slender cytoplasmic projections, which extend from the leading edge of migrating cells.

Cytonemes

Actin-based filopodial-cell extensions.

Nanotubules

Cytonemes that connect blood cells over a distance of several cell diameters and transport membrane proteins, lipids and ions from one of the connected cells to another, thus executing long range intercellular communications.

Glans penis

Sensitive tip of the penis. When the penis is flaccid it is wholly or partially covered by the foreskin, except in men who have been circumcised.

Rights and permissions

About this article

Cite this article

Hladik, F., McElrath, M. Setting the stage: host invasion by HIV.Nat Rev Immunol 8, 447–457 (2008). https://doi.org/10.1038/nri2302

Download citation

This article is cited by