A hemi-fission intermediate links two mechanistically distinct stages of membrane fission (original) (raw)

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Acknowledgements

We thank J. Chappie for helpful discussions, A. Mohanakrishnan and D. Reed for technical assistance. S.L.S. was supported by National Institutes of Health grant R01-GM42455 and the Welch Foundation Grant I-1823. V.A.F. was supported by grants from the Spanish Ministry of Economy and Competitiveness BFU2012-34885, the Basque Government Program Etortek IE12-332 and European FEDER funds. J.E.H. was supported by the National Institute of Diabetes and Digestive and Kidney Diseases Intramural Research Program. M.F. and M.M. were supported by the Volkswagen foundation and the DFG-CRC803 “Functionality controlled by organization in and between membranes” (B03). Simulations were performed at the Jülich Supercomputing Center and the North-German Supercomputer Alliance Hannover/Berlin. J.-P.M. was supported by a postdoctoral research grant from the Academy of Finland.

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Author notes

  1. Juha-Pekka Mattila and Anna V. Shnyrova: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Cell Biology, UT Southwestern Medical Center, Dallas, 75201, Texas, USA
    Juha-Pekka Mattila & Sandra L. Schmid
  2. UPV/EHU) and Department of Biochemistry and Molecular Biology, Biophysics Unit (CSIC, University of The Basque Country, Leioa, 48940, Spain
    Anna V. Shnyrova, Eva Rodriguez Hortelano & Vadim A. Frolov
  3. Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, 20892, Maryland, USA
    Anna C. Sundborger & Jenny E. Hinshaw
  4. Institute for Theoretical Physics, Georg-August University, Göttingen, 37077, Germany
    Marc Fuhrmans & Marcus Müller
  5. Department of Cell Biology, The Scripps Research Institute, La Jolla, 92037, California, USA
    Sylvia Neumann
  6. IKERBASQUE, Basque Foundation of Science, Bilbao, 48011, Spain
    Vadim A. Frolov

Authors

  1. Juha-Pekka Mattila
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  2. Anna V. Shnyrova
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  3. Anna C. Sundborger
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  4. Eva Rodriguez Hortelano
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  5. Marc Fuhrmans
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  6. Sylvia Neumann
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  7. Marcus Müller
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  8. Jenny E. Hinshaw
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  9. Sandra L. Schmid
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  10. Vadim A. Frolov
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Contributions

J.-P.M. and S.L.S. initiated the project. J.-P.M. designed and generated the CC- and CXC-Dyn1 mutants and performed all of the FRET and PET analysis, as well as their biochemical and functional characterization on liposomes and SUPER templates. A.V.S., E.R.H. and V.A.F. performed the nanotube conductance and GUV experiments. S.N. characterized the P294 mutants. A.C.S. and J.E.H. performed the cryo-EM and negative-stain EM analyses. M.F. and M.M. performed the molecular simulations. All authors discussed and interpreted the experimental data and the results of molecular simulation. S.L.S. and V.A.F. coordinated the project and wrote the manuscript, with considerable contributions from all co-authors who also approved the final version.

Corresponding authors

Correspondence toJenny E. Hinshaw, Sandra L. Schmid or Vadim A. Frolov.

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The authors declare no competing financial interests.

Extended data figures and tables

Extended Data Figure 1 Domain structure and biochemical characterization of dynamin constructs.

a, Domain structure of dynamin and cartoon illustrating that the GTPase domain (G domain, blue) connects through the bundle signalling element (BSE), composed of the N- and C-terminal helices of the G domain and the C-terminal helix from GED (yellow) to the stalk formed by the middle domain and GED (magenta). The pleckstrin homology domain (PHD, green) interacts with membrane lipids. b, c, Basal (b) and assembly-stimulated (c) rates of GTP hydrolysis for 0.5 μM WT-Dyn1 and CW-Dyn1 before and after BODIPY conjugation. Data are shown as average ± s.d., n = 3.

Extended Data Figure 2 Role of P294 in BSE conformational dynamics.

a, Changes in emission intensity of BODIPY-labelled CW-Dyn1 and CW-Dyn1(P294A) due to loss of PET after addition of 1 mM GMPPCP. Although the BSE partially opens upon addition of GMPPCP, its movements are constrained relative to wild type by the mutation of P294. b, Assembly-stimulated GTPase activity of 0.5 μM P294A, P294G and P294V Dyn1 measured on 100 nm liposomes relative to WT-Dyn1. The mutants show near wild-type activity, indicating their ability to self-assemble onto and tubulate liposomes (data shown as average ± s.d., n = 4). c, Fission activity of 0.5 μM P294A, P294G and P294V Dyn1 relative to WT-Dyn1 measured as the percentage of total membrane released from SUPER templates during 30 min incubation in the presence of GTP (data shown as average ± s.d., n = 3). Substitution of P294 with the more rigid valine residue has a greater effect on fission activity.

Extended Data Figure 3 Characterization of CxC-Dyn1.

a, Concentration dependence of the specific GTPase hydrolysis rates of WT-Dyn1 (filled squares), CC-Dyn1 (filled circles) and CxC-Dyn1 (open triangles) measured in solution at 1 mM GTP (data shown as average ± s.d., n = 3). b, EM micrographs (representative images from four independently prepared samples) showing CxC-Dyn1 assembled into rings and short spirals (arrows) in the presence of GMPPCP visualized by negative stain. Insets: top view, rings; side view, short spirals (arrows). These rings are reminiscent of those previously observed with WT-Dyn1 only in the presence of transition state nucleotide analogues (for example, GDP·AlF4−)39. Unlike CxC-Dyn1, CC-Dyn1 remained unassembled in the presence of GMPPCP (data not shown). Scale bars, 100 nm.

Extended Data Figure 4 Negative-stain and cryo-EM images of CC- and CxC-Dyn1 assembled onto PS liposomes in the absence of nucleotides.

a, b, Negative-stain (a) and cryo-EM (b) images are shown. Note the disordered nature of CxC-Dyn1 spirals relative to CC-Dyn1 structures seen via negative stain in a. Scale bars, 100 nm. b, Arrow points to relatively ordered CxC-Dyn1 assemblies, while arrowheads point to sparse dynamin assemblies appearing as single or double rings.

Extended Data Figure 5 Altered membrane interactions of the CxC-Dyn1 transition-state conformer.

a, Fluorescence emission spectra of 0.1 μM CC-Dyn1 or CxC-Dyn1 (donor) as well as dansyl-PE (acceptor)-containing liposomes (5 μM total lipid; 90 mol% PS, 10 mol% dansyl-PE) upon excitation at 280 nm. FRET between the PH domain Trp residues and dansyl is evident in the donor plus acceptor samples as a decrease in donor and an increase in acceptor emission. b, Self-assembly of the indicated proteins (1 μM) on liposomes identical to those used in the GTPase assay (300 μM total lipid; Fig. 3f) examined by sedimentation followed by SDS–PAGE analysis of the supernatant (S) and pellet (P) fractions. c, Percentages of proteins pelleted after incubation with or without 400 nm PS liposomes (1 μM protein, 300 μM total lipid) and 1 mM GTP, as indicated, was quantified by sedimentation followed by SDS–PAGE and densitometric analyses of the protein levels in supernatant and pellet fractions (data shown are average ± s.d., n = 3).

Extended Data Figure 6 Coarse-grained approach to modelling localized membrane constriction by CxC-Dyn1.

a, Schematic representation of the geometry of the ring system used to produce local constriction of a prototype membrane tube. Two pairs of rings are shown, each formed by two closely juxtaposed rings (separated by a small distance Δ_x_). The inner ring in each pair has the radius r and the outer ring has a slightly larger radius r+Δ_r_ so that the ring pair promotes creation of an hourglass membrane shape. The PHDs of dynamin are represented as amphiphilic disks evenly distributed over the rings with the centre of mass of each disk being restrained to a position on the ring (marked by blue and orange points). Two overlapping disks (purple and brown) attached to the right juxtaposed ring pair are shown. The orientations of the disks are not fixed, so the normal to the disk surface (purple and brown arrows) can have an arbitrary direction. b, Axial cross-section of a stable hemi-fission intermediate, the cylindrical micelle, created by the ring system shown in a. The rectangular box indicates the dimensions of the cylindrical micelles (the diameter D and the length L).

Extended Data Figure 7 Molecular simulations of the hemi-fission and fission transformations.

The red box shows a representative sequence of simulation snapshots (axial cross-sections) demonstrating the formation of the stable hemi-fission intermediate26. Radial constriction of a membrane tube resulted in reversible closure of the tube lumen, that is, flicker26, followed by formation of a stable cylindrical micelle structure. The blue box summarizes the simulation runs exploring the stability of the hemi-fission intermediate and its rupture. The top part shows stable structures corresponding to the constrained intermediate (left, taken at zero tension) and the unconstrained intermediate (right, taken at 0.06 dyn cm−1 tension). The bottom part shows the rupture of the intermediates by 0.6 dyn cm−1 tension (left and right) or by elongation of the ring system at 0.06 dyn cm−1 tension (middle). The characteristic times for the rupture are indicated near the corresponding blue arrows.

Extended Data Figure 8 Dynamin-catalysed membrane fission occurs in two mechanistically distinct stages through a hemi-fission intermediate.

Model overlaying the distinct dynamin activities and conformational changes onto the two energy barriers (green curve) that must be overcome, first to catalyse formation of the metastable hemi-fission intermediate and subsequently to drive full fission. When trapped in the transition state and in the absence of GTP, CxC-Dyn1 can drive the formation of a metastable and flickering hemi-fission state (solid blue curve) through the assembly of small scaffolds and enhanced wedging activity of the PHD. However, without subsequent GTPase-driven conformational changes required to loosen the scaffold, generate axial force and retract the PHD, as occurs for WT-Dyn1 (dotted blue line), the membrane-bound CxC-Dyn1 creates an insurmountable barrier to fission (dashed blue line).

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Mattila, JP., Shnyrova, A., Sundborger, A. et al. A hemi-fission intermediate links two mechanistically distinct stages of membrane fission.Nature 524, 109–113 (2015). https://doi.org/10.1038/nature14509

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