Energy Metabolism Research Papers - Academia.edu (original) (raw)

Population exposed to chronic undernutrition in early life seems to be more susceptible to obesity in adulthood due to the development of mechanisms that improve the efficiency of energy use. Therefore, these individuals have relatively... more

Population exposed to chronic undernutrition in early life seems to be more susceptible to obesity in adulthood due to the development of mechanisms that improve the efficiency of energy use. Therefore, these individuals have relatively reduced energy requirements (thrifty phenotype). To investigate, among women living on severe socioeconomic vulnerability, whether short stature, a marker for undernutrition in early life, is associated with excess body weight but not with a high energy intake. This cross-sectional study, carried out between July and November 2008, evaluated 1308 women from all (N = 39) Quilombola communities of Alagoas. Adequacy of energy intake was estimated by the ratio between energetic ingestion and the estimated energy requirement (EER). The prevalence of short stature (≤ 154.8 cm) was 43.0% and 52.4% had excess body weight (body mass index ≥ 25 kg/m(2)), being that 33.1% were overweight and 19.3% obese. Excess body weight was higher among women with short stat...

Numerous studies indicate an inflammatory link between obesity and type 2 diabetes. The inflammatory kinases IKKɛ and TBK1 are elevated in obesity; their inhibition in obese mice reduces weight, insulin resistance, fatty liver and... more

Numerous studies indicate an inflammatory link between obesity and type 2 diabetes. The inflammatory kinases IKKɛ and TBK1 are elevated in obesity; their inhibition in obese mice reduces weight, insulin resistance, fatty liver and inflammation. Here we studied amlexanox, an inhibitor of IKKɛ and TBK1, in a proof-of-concept randomized, double-blind, placebo-controlled study of 42 obese patients with type 2 diabetes and nonalcoholic fatty liver disease. Treatment of patients with amlexanox produced a statistically significant reduction in Hemoglobin A1c and fructosamine. Interestingly, a subset of drug responders also exhibited improvements in insulin sensitivity and hepatic steatosis. This subgroup was characterized by a distinct inflammatory gene expression signature from biopsied subcutaneous fat at baseline. They also exhibited a unique pattern of gene expression changes in response to amlexanox, consistent with increased energy expenditure. Together, these data suggest that dual-...

The colorectal cancer (CRC) is the second cause of death in the entire population of oncological patients. Despite of the development of diagnostic methods, screening programs, monitoring of patients after surgery or chemotherapy, results... more

The colorectal cancer (CRC) is the second cause of death in the entire population of oncological patients. Despite of the development of diagnostic methods, screening programs, monitoring of patients after surgery or chemotherapy, results of survival rates are not satisfactory. The reason is that frequently the detection of the disease takes place already at the advanced stage, as every third patient at the time of diagnosis of CRC has already metastases. CEA and CA 19-9 markers are commonly used to detect cancer and to monitor the disease recurrence, are not sufficient. For this reason it becomes necessary to introduce new additional markers that will enable a higher sensitivity and specificity for detecting cancer, Medycyna fotodynamiczna / Photodynamic Medicine Acta Bio-Optica et Informatica Medica Inżynieria Biomedyczna, vol. 19, nr 2, 2013 149 it’s recurrence and progression and will be helpful to predict the course of disease and response to standard therapy. Basing on an adva...

Anaerobic glycolysis is a major source of energy for activity in reptiles. In this experiment, lactate and premature lactate accumulation in house gecko (Hemidactylus frenatus) was measured by the time it took the geckos to reach... more

Anaerobic glycolysis is a major source of energy for activity in reptiles. In this experiment, lactate and premature lactate accumulation in house gecko (Hemidactylus frenatus) was measured by the time it took the geckos to reach exhaustion, righting response and change in respiration rates. Lactate accumulation was measured after extensive exercise on the treadmill and injection of 0.9% NaCl (saline) solution, while premature lactate accumulation was measured after injection 80 mM solution of lactic acid which was adjusted to 300 mOSM of NaCl (lactate) solution. The result showed that the null hypothesis was rejected and significant differences were found among the three groups. Further test determined that significant differences were found during the time it took for the geckos to reach exhaustion. The differences were among the lactate injection and no injection group, and the lactate and saline injection group.

OBJECTIVE: The goal was to explore the effects of duration and regularity of sleep schedules on BMI and the impact on metabolic regulation in children. METHODS: Sleep patterns of 308 community-recruited children 4 to 10 years of age were... more

OBJECTIVE: The goal was to explore the effects of duration and regularity of sleep schedules on BMI and the impact on metabolic regulation in children. METHODS: Sleep patterns of 308 community-recruited children 4 to 10 years of age were assessed with wrist actigraphs for 1 week in a cross-sectional study, along with BMI assessment. Fasting morning plasma levels of glucose, insulin, lipids, and high-sensitivity C-reactive protein also were measured for a subsample. RESULTS: Children slept 8 hours per night, on average, regardless of their weight categorization. A nonlinear trend between sleep and weight emerged. For obese children, sleep duration was shorter and showed more variability on weekends, compared with school days. For overweight children, a mixed sleep pattern emerged. The presence of high variance in sleep duration or short sleep duration was more likely associated with altered insulin, low-density lipoprotein, and high-sensitivity C-reactive protein plasma levels. Child...

We tested the hypothesis that an acute decrease in muscle TCA cycle intermediates during contraction would compromise aerobic energy delivery. Male Wistar rats were anaesthetized and the gastrocnemius-plantaris-soleus (GPS) muscle complex... more

We tested the hypothesis that an acute decrease in muscle TCA cycle intermediates during contraction would compromise aerobic energy delivery. Male Wistar rats were anaesthetized and the gastrocnemius-plantaris-soleus (GPS) muscle complex from one leg was isolated and perfused with a red cell medium containing either saline (Con) or cycloserine (Cyclo; 0.05 mg g-1), an inhibitor of alanine aminotransferase (AAT). After 1 h of perfusion, the GPS muscle was either snap frozen (Con-Rest, n=11; Cyclo-Rest, n=9) or stimulated to contract for 10 min (1 Hz, 0.3 ms, 2 V) with blood flow fixed at 30 ml min-1 (100 g)-1 and then snap frozen (Con-Stim, n=10; Cyclo-Stim, n=10). Maximal AAT activity was>80% lower (P<0.001) in both Cyclo-treated groups (Rest: 0.61+/-0.02; Stim: 0.63+/-0.01 mmol (kg wet wt)-1 min-1; mean+/-s.e.m.) compared to Con (Rest: 3.56+/-0.16; Stim: 3.92+/-0.29). The sum of five measured TCAI (SigmaTCAI) was reduced by 23% in Cyclo-Rest versus Con-Rest but this was not ...

BACKGROUND: The effects of humic substances (HS) on quality traits (colour, water‐holding capacity, and pH) of breast and thigh meats and on blood minerals (calcium and phosphorus) and on metabolites (glucose, total protein, triglyceride... more

BACKGROUND: The effects of humic substances (HS) on quality traits (colour, water‐holding capacity, and pH) of breast and thigh meats and on blood minerals (calcium and phosphorus) and on metabolites (glucose, total protein, triglyceride and cholesterols) as well as on the performance, carcass, and gastrointestinal tract (gut) traits of broilers were studied. In total, 480 male chicks (ROSS 308) were allocated to four treatments, each with five replicates containing 24 birds. While control birds were fed a diet (mash form) with no additives (0 HS), other treatment birds were fed with diets containing HS at 0.5 (0.5 HS), 1.0 (1 HS) or 1.5 (1.5 HS) g kg−1 from 1 to 42 days of age.RESULTS: The body and carcass weights and feed efficiency increased (P < 0.05) by 1.5 HS, and blood cholesterol decreased (P < 0.05) by 1 HS and 1.5 HS compared to 0 HS. The 0.5 HS decreased (P < 0.10) low‐density lipoprotein compared to 0 HS. The 0.5 HS resulted in thigh meat and breast meat that we...

Environmental temperature can act as a positive or negative modulator of the physiology and metabolism of poikilothermic organisms. As a general rule, larvae and juveniles are more sensitive to temperature stress than adults, which... more

Environmental temperature can act as a positive or negative modulator of the physiology and metabolism of poikilothermic organisms. As a general rule, larvae and juveniles are more sensitive to temperature stress than adults, which represents a key factor that partly determines their development and growth in aquaculture. Therefore, this study analyzed the effect of exposure to temperatures of 21, 24, and 27°C on the respiratory metabolism (RM) of Totoaba macdonaldi in different developmental stages. For this purpose, eggs, larvae with 4, 6, 8, 14, and 22 days post-hatch (dph), and juveniles of 25 dph were exposed to the experimental temperatures for 5 h. After the exposure time, oxygen consumption measurements were performed. The results clearly show that temperature (21 to 27°C) has the greatest effect on RM in eggs and larvae at 4 and 22 dph (3.1 ± 0.3 to 4.3 ± 0.3 μmol O2 h-1 egg-1, 2.9 ± 0.3 to 10.5 ± 1.2 μmol O2 h-1 larvae-1 and 102.0 ± 6.4 to 189.8 ± 15.3 μmol O2 h-1 larvae-1...

Helicobacter pylori, is an invariably commensal resident of the gut microbiome associated with gastric ulcer in adults. In addition, these patients also suffered from a low grade inflammation that activates the immune system and thus... more

Helicobacter pylori, is an invariably commensal resident of the gut microbiome associated with gastric ulcer in adults. In addition, these patients also suffered from a low grade inflammation that activates the immune system and thus increased shunting of energy to host defense mechanisms. To assess whether a H. pylori infection could affect growth in early life, we determined the expression levels of selected metabolic gut hormones in germ free (GF) and specific pathogen-free (SPF) mice with and without the presence of H. pylori. Despite H. pylori-infected (SPFH) mice display alteration in host metabolism (elevated levels of leptin, insulin and peptide YY) compared to non-infected SPF mice, their growth curves remained the same. SPFH mice also displayed increased level of eotaxin-1. Interestingly, GF mice infected with H. pylori (GFH) also displayed increased levels of ghrelin and PYY. However, in contrast to SPFH mice, GFH showed reduced weight gain and malnutrition. These prelimi...

Background Physical activity patterns of a population remain mostly assessed by the questionnaires. However, few physical activity questionnaires have been validated in Asian populations. We previously utilized a combination of different... more

Background Physical activity patterns of a population remain mostly assessed by the questionnaires. However, few physical activity questionnaires have been validated in Asian populations. We previously utilized a combination of different questionnaires to assess leisure time, transportation, occupational and household physical activity in the Singapore Prospective Study Program (SP2). The International Physical Activity Questionnaire (IPAQ) has been developed for a similar purpose. In this study, we compared estimates from these two questionnaires with an objective measure of physical activity in a multi-ethnic Asian population. Methods Physical activity was measured in 152 Chinese, Malay and Asian Indian adults using an accelerometer over five consecutive days, including a weekend. Participants completed both the physical activity questionnaire in SP2 (SP2PAQ) and IPAQ long form. 43subjects underwent a second set of measurements on average 6 months later to assess reproducibility o...

Multiple infarcts were produced in cerebral hemispheres of rats by injecting calibrated 50-micron microspheres into the left internal carotid artery, and alterations in lipid and energy metabolism were evaluated 24 hours later in the... more

Multiple infarcts were produced in cerebral hemispheres of rats by injecting calibrated 50-micron microspheres into the left internal carotid artery, and alterations in lipid and energy metabolism were evaluated 24 hours later in the embolized hemisphere. Total phospholipid content was decreased by 26%, but the different classes of phospholipids were not equally affected. Phosphatidylinositol and phosphatidylserine levels were decreased by about 40% and phosphatidylcholine and phosphatidylethanolamine by 25%, while sphingomyelin level remained unchanged. There was a 3.2-fold increase in total free fatty acid content with a relatively larger rise in polyunsaturated free fatty acids 20:4 and 22:6 (20-fold increase). Determination of enzyme activities showed decreases in Na+,K+-ATPase (-21%) and hexokinase (-14%) but no changes in phosphofructokinase and pyruvate kinase. Study of energy metabolism using the closed system method of Lowry et al showed a significant depression (-36%) of t...

Chronic glucocorticoid use for treatment of inflammatory diseases is accompanied by severe side effects in humans (e.g. hyperglycemia and insulin resistance). The present studies were conducted to characterize consequences of chronic... more

Chronic glucocorticoid use for treatment of inflammatory diseases is accompanied by severe side effects in humans (e.g. hyperglycemia and insulin resistance). The present studies were conducted to characterize consequences of chronic treatment with the synthetic glucocorticoid prednisolone on insulin sensitivity and blood glucose kinetics in mice. Prednisolone treatment increased fasting blood glucose and plasma insulin concentrations, but this apparently reduced insulin sensitivity could not be confirmed in hyperinsulinemic euglycemic clamp studies. Therefore, a novel method to study whole body glucose kinetics was used. This method revealed that prednisolone-treated mice show an increased hepatic glucose production (HGP). The increased HGP was accompanied by elevated plasma insulin concentrations, indicating reduced insulin sensitivity of hepatic glucose metabolism in prednisolone-treated mice. Compared with vehicle, prednisolone-treated mice had lower blood glucose concentrations...